Effect of cobalt and chromium ions on human MG-63 osteoblasts in vitro: Morphology, cytotoxicity, and oxidative stress

Fleury, Cyrille; Petit, Alain; Mwale, Fackson; Antoniou, John; Zukor, David J.; Tabrizian, Maryam; Huk, Olga L.

doi:10.1016/j.biomaterials.2006.01.035

Cited by 156 publications

(149 citation statements)

References 54 publications

Supporting

Mentioning

134

Contrasting

Unclassified

Order By: Relevance

“…Cobalt ions have been shown to lead to apoptosis and a decrease in cell viability in macrophages and in osteoblasts over the first 24 h of exposure, the interval used in our experiments. 33,37,38 It is possible that our observed chemokine secretion and decrease in osteoblast function occurs in the context of an overall cytotoxic effect of cobalt ions on osteoblasts.…”

Section: Discussionmentioning

confidence: 96%

“…28,29 Cytotoxic effects of cobalt ions have been demonstrated in macrophages, with cobalt ions inducing apoptosis, necrosis, and TNF-a secretion. [30][31][32][33][34] A similar cytotoxic effect has been observed in osteoblast-like cells, with reduced osteoblast proliferation, [35][36][37] viability, 35,37 and function 35,36,38 observed. In this study we investigated the in vitro effect of cobalt ions on primary human osteoblasts (pHOBs).…”

mentioning

confidence: 77%

See 1 more Smart Citation

Cobalt ions induce chemokine secretion in primary human osteoblasts

Queally¹,

Devitt²,

Butler³

et al. 2009

Journal Orthopaedic Research

View full text Add to dashboard Cite

Chemokines are major regulators of the inflammatory response and have been shown to play an important role in periprosthetic osteolysis. Titanium particles have previously been shown to induce IL-8 and MCP-1 secretion in osteoblasts. These chemokines result in the chemotaxis and activation of neutrophils and macrophages, respectively. Despite a resurgence in the use of cobalt-chromium-molybdenum alloys in metal-on-metal arthroplasty, cobalt and chromium ion toxicity in the periprosthetic area has been insufficiently studied. In this study we investigate the in vitro effect of cobalt ions on primary human osteoblast activity. We demonstrate that cobalt ions rapidly induce the protein secretion of IL-8 and MCP-1 in primary human osteoblasts. This elevated chemokine secretion is preceded by an increase in the transcription of the corresponding chemokine gene. Using a Transwell migration chemotaxis assay we also demonstrate that the chemokines secreted are capable of inducing neutrophil and macrophage migration. Furthermore, cobalt ions significantly inhibit osteoblast function as demonstrated by reduced alkaline phosphatase activity and calcium deposition. In aggregate these data demonstrate that cobalt ions can activate transcription of the chemokine genes IL-8 and MCP-1 in primary human osteoblasts. Cobalt ions are not benign and may play an important role in the pathogenesis of osteolysis by suppressing osteoblast function and stimulating the production and secretion of chemokines that attract inflammatory and osteoclastic cells to the periprosthetic area. ß

show abstract

Section: Discussionmentioning

confidence: 96%

mentioning

confidence: 77%

Cobalt ions induce chemokine secretion in primary human osteoblasts

Queally¹,

Devitt²,

Butler³

et al. 2009

Journal Orthopaedic Research

View full text Add to dashboard Cite

show abstract

“…Although the metal ion concentrations within bone in vivo has not been established, exposure of osteoblasts to Co particles at concentrations of 100 µg/L or higher leads to the development of cytoplasmic vacuolations (Allen et al 1997, Anissian et al 2002, Hallab et al 2002. Moreover, Co and Cr ions are toxic for osteoblasts, leading to markedly reduced alkaline phosphatase activity (McKay et al 1996, Fleury et al 2006). Co and Cr ions induce oxidation and nitration of proteins and dysregulation of the expression of antioxidant enzymes (Fleury et al 2006).…”

Section: Osteoblastsmentioning

confidence: 99%

“…Moreover, Co and Cr ions are toxic for osteoblasts, leading to markedly reduced alkaline phosphatase activity (McKay et al 1996, Fleury et al 2006). Co and Cr ions induce oxidation and nitration of proteins and dysregulation of the expression of antioxidant enzymes (Fleury et al 2006). The cells are capable of releasing proinflammatory cytokines into the microenvironment, such as IL-6 and TNF-α (Hallab et al 2001, Anissian et al 2002, Hallab et al 2002.…”

Section: Osteoblastsmentioning

confidence: 99%

Metal-on-metal hip resurfacing arthroplasty: A review of periprosthetic biological reactions

et al. 2008

View full text Add to dashboard Cite

“…Periprosthetic osteolysis has also been associated with a perivascular accumulation of activated macrophages and T-lymphocytes producing bone-resorbing cytokines (Park et al, 2005). In addition, Co and Cr are toxic to osteoblasts, leading to markedly reduced alkaline phosphatase activity (McKay et al, 1996;Fleury et al, 2006). The cells can also release pro-inflammatory cytokines such as IL-6 and TNF- (Hallab, 2001;Anissian et al, 2002;Hallab et al, 2002).…”

Section: Metallurgymentioning

confidence: 99%

Hip resurfacing arthroplasty: current status and future perspectives

Corten

Ganz²,

Simon³

et al. 2011

eCM

View full text Add to dashboard Cite

Hip resurfacing arthroplasty (HRA) is a concept of hip replacement that allows treating young active patients with a femoral bone preserving procedure. The proposed advantages of resuming an active lifestyle with increased frequency and duration of sports activities have been shown to be realistic. The 30-year cost-effectiveness in young male patients has been shown to be higher in resurfacing compared to conventional total hip replacement (THA). However, prognosticators of an inferior outcome have also been identified. The most important patient related factors are secondary osteoarthritis as the indication for surgery such as post-childhood hip disorders or AVN, female gender, smaller component sizes and older age (>65 years for males and >55 years for females). In addition, surgical technique (approach and cementing technique) and component design are also important determinant factors for the risk of failure. Moreover, concerns have surfaced with respect to high metal ion concentrations and metal ion hypersensitivities. In addition, the presumed ease of revising HRA has not reflected in improved or equal survivorship in comparison to a primary THA. This highlights the importance of identifying patient-, surgery-, and implant-related prognosticators for success or failure of HRA. Rather than vilifying the concept of hip resurfacing, detailed in depth analysis should be used to specify indications and improve implant design and surgical techniques.

show abstract

Effect of cobalt and chromium ions on human MG-63 osteoblasts in vitro: Morphology, cytotoxicity, and oxidative stress

Cited by 156 publications

References 54 publications

Cobalt ions induce chemokine secretion in primary human osteoblasts

Cobalt ions induce chemokine secretion in primary human osteoblasts

Metal-on-metal hip resurfacing arthroplasty: A review of periprosthetic biological reactions

Hip resurfacing arthroplasty: current status and future perspectives

Contact Info

Product

Resources

About