Effect of Cobalt on Methanogenesis

Bhattacharya, Sanjoy K.; Uberoi, Vikas; Madura, Richard L.; Haghighi-Podeh, Mohammad R.

doi:10.1080/09593331608616269

Cited by 25 publications

(12 citation statements)

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“…Cobalt ion is required for nitrogen metabolism because of 3-dehydroquinate synthase activity dependent on the concentration of cobalt ions. The proposed cobalt assimilation systems is accorded with earlier investigations (Lorowitz et al 1992;Bhattacharya et al 1995;Brindley et al 2003).…”

Section: Discussionsupporting

confidence: 87%

In silico description of cobalt and nickel assimilation systems in the genomes of methanogens

Chellapandi

2011

Syst Synth Biol

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Methanogens are a diverse group of organisms that can live in a wide range of environments. Herein, cobalt and tungsten assimilation pathways have proposed to be established in the genomes of Methanococcus maripaludies C5 and Methanosarcina mazei Go1, respectively. All of the proteins involved in the proposed pathways were identified from public domain databases and then complied manually to reconstruct the pathways. The function of proteins with unknown function was assigned by a combined prediction approach. Totally, 17 proteins were identified to cobalt transport and assimilation processes whereas 7 proteins reported to tungsten assimilation system. Phylogenetic analysis of this study revealed that heavy metal transporter of methanogens could be evolved from closely related members in the different genera of methanogens. Nevertheless, genes encoding for metal resistance proteins could be originated from thermophilic and sulfur reducing bacteria. Many metalloenzymes in methanogens were very unique to the species of methanogens. It implied that these metal ions were utilized to produce the precursors for energy driven processes of methanogens. This study suggested that in combination of systems models and evolutionary inference can only correlate metabolic fluxes and physiological changes in methanogens. In silico models of this study will provide insights to design experiments for heavy metal assimilation processes of methanogens growing under heavy metal-rich environments and or in a laboratory condition.

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Section: Discussionsupporting

confidence: 87%

In silico description of cobalt and nickel assimilation systems in the genomes of methanogens

Chellapandi

2011

Syst Synth Biol

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“…Similarly, Kungolos [24] demonstrated a decrease of bioavailability (and therefore also toxicity) of heavy metals in the presence of increasing amounts of humic acids. For dissolved cobalt, the IC 50 values reported in the present study (Table 5) Bhattacharya et al [4] studied cobalt toxicity to anaerobic sludge in semi-continuous reactors under different experimental conditions: suspended enrichment culture, different medium composition (higher concentrations of inorganic salts) and different substrates (acetate and glucose). They observed an approximately 50 times higher IC 50 value for the free cobalt ion (approximately 750 and 950 lmol/L for glucose and acetate, respectively) compared to the present study.…”

Section: Role Of Speciation In Cobalt Toxicitymentioning

confidence: 66%

“…As cobalt toxicity is mainly due to interaction with divalent physiological ions (the ions having physiological functions), mainly Fe 2? [29], the higher iron concentration (0.2 mmol/L) used by Bhattacharya et al [4] probably also decreased the free cobalt toxicity. The different methods for free cobalt concentration measurement (a combination of dialysis and ion exchange methods) used by Bhattacharya et al [4] could also affect the conclusions.…”

Section: Role Of Speciation In Cobalt Toxicitymentioning

confidence: 88%

“…The higher value for the supplied cobalt is probably due to higher salt concentrations (e.g. 60 mM HCO 3 -) applied by Bhattacharya et al [4], which lead to more cobalt precipitation and complexation. The high free cobalt IC 50 value is likely due to toxicity prevention by the presence of higher calcium and magnesium concentration (0.17 mM Ca 2?…”

Section: Role Of Speciation In Cobalt Toxicitymentioning

confidence: 99%

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Cobalt toxicity in anaerobic granular sludge: influence of chemical speciation

Bartacek

Fermoso

Baldó-Urrutia

et al. 2008

J Ind Microbiol Biotechnol

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The influence of cobalt speciation on the toxicity of cobalt to methylotrophic methanogenesis in anaerobic granular sludge was investigated. The cobalt speciation was studied with three different media that contained varying concentrations of complexing ligands [carbonates, phosphates and ethylenediaminetetraacetic acid (EDTA)]. Three fractions (nominal added, dissolved and free) of cobalt were determined in the liquid media and were correlated with data from batch toxicity experiments. The average concentration of cobalt that was required for 50% inhibition of methanogenic activity (IC 50 ) for free Co 2? in the three sets of measurements was 13 lmol/L with a standard deviation of 22% and a similarity of 72% between the data obtained in the three different media for the range of cobalt concentrations investigated. The standard deviation of the IC 50 for the other two fractions was much higher, i.e. 85 and 144% for the added cobalt and dissolved cobalt, respectively, and the similarity was almost 0% for both fractions. Complexation (and precipitation) with EDTA, phosphates and carbonates was shown to decrease the toxicity of cobalt on methylotrophic methanogenesis. The free cobalt concentration is proposed to be the key parameter to correlate with cobalt toxicity. Thus, the toxicity of cobalt to granular sludge can be estimated based on the equilibrium-free cobalt concentration.

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“…Although the specific activity of biomass depends on many factors, the lack of a single trace element can severely limit the overall process (Speece, 1996). On the other hand, a higher concentration may lead to toxicity, due to the formation of unspecific complex compounds in the cell (Nies, 1999), resulting in, e.g., inhibition of methanogens (Bhattacharya et al, 1995;Fathepure, 1987). The effect of cobalt on the anaerobic degradation of methanol under mesophilic conditions has been studied by Florencio et al (1993Florencio et al ( , 1994.…”

Section: Introductionmentioning

confidence: 98%

Effect of cobalt on the anaerobic thermophilic conversion of methanol

Paulo

Jiang

Cysneiros

et al. 2004

Biotech & Bioengineering

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The importance of cobalt on the anaerobic conversion of methanol under thermophilic conditions was studied in three parallel lab-scale UASB-reactors and in cobalt-limited enriched cultures. Reactors R1, R2, and R3 were fed with methanol in a bicarbonate-buffered medium, supplied with iron and macronutrients: in R1 all metals were supplied (control), R2 was cobalt deprived, and in R3 all metals were deprived. In the 136 days of continuous experiment, a drop in performance was observed over the last 30 days. Particularly in R3, both methanol removal and methane formation dropped by 7.1% and 13.7%, respectively, compared to the control reactor, R1. When the medium was cobalt-deprived, acetate was not produced and, as a consequence, the enriched consortium lost its capacity to degrade acetate, indicating that the acetotrophic microorganisms were washed out. The addition of 0.5 microM of cobalt to a cobalt-deprived enrichment culture led to acetate accumulation. The results obtained in this study indicate that the mixed consortium requires a proper amount of cobalt, and its addition to a concentration of 0.1 microM leads to the highest methanol conversion rate, with methane as the sole end product from methanol.

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Effect of Cobalt on Methanogenesis

Cited by 25 publications

References 0 publications

In silico description of cobalt and nickel assimilation systems in the genomes of methanogens

In silico description of cobalt and nickel assimilation systems in the genomes of methanogens

Cobalt toxicity in anaerobic granular sludge: influence of chemical speciation

Effect of cobalt on the anaerobic thermophilic conversion of methanol

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