Biofertilizers are eco friendly and are now most, necessary to support developing organic agriculture and sustainable agriculture. They can provide to the small and marginal farmers an ecofriendly viable weapon to attain the ultimate goal of increasing crop productivity. Cyanobacteria also called blue green algae which are useful to mankind in various ways and constitute a vast potential resource in varied application such as food, feed, fuel, fertilizer, medicine, industrial products and in combating pollution. Coir pith, a light lignocellulosic material which is spongy, highly hygroscopic and extremely compressive having high lignin content, accumulated in tons creating environmental problems. Hence, recycling the waste by using cyanobacteria in order to get pollution free environment and useful products from coir pith will be an economical and purposeful one. The purpose of this study was to evaluate the effect of different amount of coir pith based cyanobacterial biofertilizers named cyanopith individually on the morphological and biochemical characteristics as well as yield of Aloe barbadensis Miller (Aloe vera). The pot experiment was conducted at the Model Organic Farm of Bharathidasan University located in Tamilnadu, India. Different treatments of cyanopith (Solid form) fertilizer viz., cyanopith 25g, 50g, 75g, 100g, 125g and 150 g/pot, and the plants without any treatment were considered as control. It was observed that the plant appeared maximum height, number of leaves and leaf weight, leaf breadth, number of offsets and significant improvement in gel and latex yield, as well as Chlorophyll a, chlorophyll b, total chlorophyll, carotenoids, free amino acids and sugar contents were found to be improved with 100g cyanopith over control. Hence, it revealed that 100g of cyanopith fertilizer was the optimum concentration for maximum improvement of the plant characteristics and yield of A. barbadensis Miller.