Effect of Cold Plasma on Cell Viability and Collagen Synthesis in Cultured Murine Fibroblasts

Abstract

An argon atmospheric pressure plasma jet was employed to treat L929 murine fibroblasts cultured in vitro. Experimental results showed that, compared with the control cells, the treatment of fibroblasts with 15 s of plasma led to a significant increase of cell viability and collagen synthesis, while the treatment of 25 s plasma resulted in a remarkable decrease. Exploration of related mechanisms suggested that cold plasma could up-regulate CyclinD1 gene expression and down-regulate p27 gene expression at a low dose, while it could down-regulate CyclinD1 expression and up-regulate p27 expression at a higher dose, thus altering the cell cycle progression, and then affecting cell viability and collagen synthesis of fibroblasts.

Cold atmospheric-pressure air plasma treatment of C6 glioma cells: effects of reactive oxygen species in the medium produced by the plasma on cell death

“…Nitrogen oxides generated from the air plasma dissolve in the liquid forming nitrites and nitrates in the liquid by the reaction ( (aq) means an aqueous species) [24][25][26] It is difficult to detect the concentration of short-lived reactive species in the plasma-treated liquid (PBS) due to the short half-life and high reactivity. However, the presence of the long-lived reactive species in the aqueous phase can provide indirect proof of the existence of short-lived reactive species according to the aforementioned reactions (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13). Figure 3(a) shows the concentrations of hydrogen peroxide, nitrate ion, and ozone in the PBS after the air plasma treatment.…”

“…Suggested as a promising approach to diseases including cancer, various types of CAPs based on different power sources and configurations have been developed because they interact with tissue or cell without a significant temperature increase [1][2][3][4][5]. In particular, the atmospheric-pressure plasma jet is often used to treat cells in vitro and in vivo [6][7][8][9][10]. It has also been demonstrated that CAPs can induce cell apoptosis or necrosis.…”

Molecular Mechanisms of the Efficacy of Cold Atmospheric Pressure Plasma (CAP) in Cancer Treatment

“…In addition to the various cell types, the TME consists of collagen, elastin, fibronectin, glycoproteins, and proteoglycan [92]. It has been observed that prolonged treatment with CAP inhibits cell viability and collagen production of murine fibroblasts [93]. A reduction in collagen secretion and the migration behavior was also observed after CAP treatment in keloid fibroblasts, which, like tumor-associated fibroblasts, show an overproduction of collagen [94,95].…”

Modifying the Tumour Microenvironment: Challenges and Future Perspectives for Anticancer Plasma Treatments

“…CAFs are considered critical players in the malignant progression with a complex bidirectional communication mechanism between CAFs and cancer cells mediated by cytokines and RNA transference via exosomes to favour metastasis, vascular permeability, and resistance to chemotherapy and radiotherapy [111][112][113]. It has been proposed that plasma has dual effects on fibroblasts, as short treatments enhanced the cell viability and collagen production, whereas longer treatments inhibit them [114]. Exposure to longer plasma treatments of higher plasma-derived ROS concentrations can induce senescence [115] and necrotic cell death in fibroblasts [116].…”