Effect of cold stress on cell membrane stability, chlorophyll a and b contain and proline accumulation in wheat (Triticum aiestivum L.) variety

Abstract

The aim of this research was to determine the effect of cold stress on chlorophyll content, proline accumulation and the cell membrane stability of 15 bread wheat (Triticum aestivum L.) at two stage of growth (2 leaf stage and 4 leaf stage). Experiment was carried out in complete block design with tree replications at year 2009 in west Azerbaijan Agriculture science and research center laboratory. 15 cultivar (five genotypes with types winters names Sayson, Martin, Gaskoghen, C-82-12, C-81-14, 5 genotype with type intermediate including Alvand, Mahdavi, Zarrin, Marvdasht, Toos and five genotypes with type spring names Shiraz, Pishtaz, M-79-7, M-81-13 and Kavir) were sown in lab condition. According to the results, cold stress had a significant effect on chlorophyll a and b, total chlorophyll, prolin content and cell membrane stability. At the other hand variety and growing stage have significant effect on treats (P>0.01). The result showed that the M-81-13 with 53.05 mΩ EC has the highest cell membrane stability and the lowest EC was obtaining from gaskoghen with 18.987 mΩ. Also M-79-7 with 2.315 mg chlorophyll a per 1 g leaf fresh weight has highest chlorophyll content. It was determining the ratio of maximal chlorophyll content after cold stress is reliable and a method to determine the frost hardness of wheat variety. The proline content of the leaf however increased in both growth stages in all variety of wheat. Gaskoghen with 0.340 (µg/0.5 g dwt) in 2 leaf growing stage had the highest rate of proline amino acid and Marvdasht variety from inter mediate type in 4 leaf growing stage with 0.003 (µg/0.5 g dwt) had the lowest rate of proline

Cold tolerance of forage plant species

“…Similar stress-induced increases in electrolyte leaching have also been reported to occur in grapevine (BERTAMINI et al, 2007), wheat (HABIBI et al, 2011), maize, and Guatemala grass (JATIMLIANSKY et al, 2004). However, Jatimliansky et al (2004) reported that the measurement of electrical conductivity was insufficiently sensitive for detecting differences in the cold tolerance of maize (Zea mays) genotypes.…”

“…This technique is also considered very versatile and highly sensitive for evaluating the tolerance of plants to stress (ARVIN;DONNELLY, 2008;MOSHTAGHI et al, 2009). Electrical conductivity has been used to quantify cell membrane damage after exposure to a variety of abiotic stressors, including cold, and has been used in a variety of crop species, including alfalfaMedicago sativa L. (SULC et al, 1991), olive -Olea europaea L. (BARRANCO et al, 2005) HABIBI et al, 2011), and various eucalyptus species -Eucalyptus spp. (FLORIANI et al, 2013).…”

Evaluation of Physiological Traits, Yield and Yield Components at Two Growth Stages in 10 Durum Wheat Lines Grown under Rainfed Conditions in Southern Syria

“…Avenson et al (2005) demonstrated that chl content is positively correlated with photosynthetic rate. Crops may increase chl content as an effective way to increase biomass production and grain yield (Habibi et al 2011).…”

Alterations in Chlorophyll a Fluorescence and Pigments Concentration in the Leaves of Cauliflower and Broccoli Transplants Subjected to Chilling

“…Decreasing chlorophyll content in plant leaves under low temperature is often considered a typical symptom of oxidative stress (Yadegari et al, 2007). This was shown, for example, in chill-sensitive coffee seedlings (Oliveira et al, 2009), different rice genotypes (Zhang et al, 2010;Perveen et al, 2013), wheat (Habibi et al, 2011), and chill-sensitive maize cultivars (Aroca et al, 2001). Partelli et al (2009) reported that coffee plants responded with a 30% reduction in chlorophyll a content and 27% reduction in chlorophyll b when the day/night temperature decreased from 25/20 to 13/8 °C.…”