Effect of collagen I and fibronectin on the adhesion, elasticity and cytoskeletal organization of prostate cancer cells

Docheva, Denitsa; Padula, Daniela; Schieker, Matthias; Clausen‐Schaumann, Hauke

doi:10.1016/j.bbrc.2010.10.034

Cited by 54 publications

(47 citation statements)

References 29 publications

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“…The maximum indentation force at each point was set to 10 nN, which resulted in an indentation depth of ∼100 nm, ensuring that only the stiffness of the first cuticular layer was probed. From each of these selected points, a force-indentation curve was produced, which was used to extract the mechanical properties of the sample surface (40). Measurements were obtained from 40 to 65 points within a 75 × 75 μm grid in the central region of the carapace of each individual, which were used to calculate the mean elastic modulus of the cuticle for each Daphnia.…”

Section: Methodsmentioning

confidence: 99%

Changes in water chemistry can disable plankton prey defenses

Riessen

Linley

Altshuler

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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The effectiveness of antipredator defenses is greatly influenced by the environment in which an organism lives. In aquatic ecosystems, the chemical composition of the water itself may play an important role in the outcome of predator–prey interactions by altering the ability of prey to detect predators or to implement defensive responses once the predator’s presence is perceived. Here, we demonstrate that low calcium concentrations (<1.5 mg/L) that are found in many softwater lakes and ponds disable the ability of the water flea, Daphnia pulex to respond effectively to its predator, larvae of the phantom midge, Chaoborus americanus. This low-calcium environment prevents development of the prey’s normal array of induced defenses, which include an increase in body size, formation of neck spines, and strengthening of the carapace. We estimate that this inability to access these otherwise effective defenses results in a 50–186% increase in the vulnerability of the smaller juvenile instars of Daphnia, the stages most susceptible to Chaoborus predation. Such a change likely contributes to the observed lack of success of daphniids in most low-calcium freshwater environments, and will speed the loss of these important zooplankton in lakes where calcium levels are in decline.

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Section: Methodsmentioning

confidence: 99%

Changes in water chemistry can disable plankton prey defenses

Riessen

Linley

Altshuler

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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“…[9][10][11] Changes in mechanical properties during pathological progressions have been investigated using AFM, 12,13) and abnormal reorganizations of actin cytoskeletons were noticed for many types of cancer cells. 14,15) Not only having an influence on the mechanical compliance, the actin cytoskeleton also affects many cellular processes such as motility, structural stability, cell division, cell-cell adhesion, cell-substrate adhesion, and membrane permeability. 16,17) These processes are crucial for cancer cells to migrate through physical and chemical barriers encountered during cancer metastasis.…”

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confidence: 99%

Nano-mechanical Reinforcement in Drug-Resistant Ovarian Cancer Cells

Seo

et al. 2015

Biological & Pharmaceutical Bulletin

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The mechanical properties of cells are considered promising biomarkers for the early detection of cancer and the testing of drug efficacy against it. Nevertheless, generalized correlations between drug resistance and the nano-mechanical properties of cancer cells are yet to be defined due to the lack of necessary studies. In this study, we conducted atomic force microscopy (AFM)-based nano-mechanical measurements of cisplatinsensitive (A2780) and cisplatin-resistant (A2780cis) ovarian cancer cells. The difference in the efficacy of cisplatin between A2780 and A2780cis was confirmed in the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. We observed that the cisplatin-resistant ovarian cancer cells were more motile than cisplatin-sensitive cells based on the results of the wound closure experiment, and the AFM experiments showed that drug resistance induced nano-mechanical stiffening of the ovarian cancer cells. Increased mechanical stiffness caused by cisplatin resistance was consistent with the confocal microscopy images showing more distinct actin stress fibers in A2780cis than in A2780 cells. The down regulation of vinculin implicated the actin-driven elongation as a major motile mode for A2780cis cells. Our results consistently indicated that the acquisition of drug resistance in ovarian cancer cells induces an extensive reorganization of the actin cytoskeleton, which governs the cellular mechanical properties, motility, and possibly intracellular drug transportation.Key words atomic force microscopy (AFM); ovarian cancer; drug resistance; Young's modulus; nanomechanics Ovarian cancer is the leading cause of cancer-related mortality in gynecological malignancies. Little or no specific symptom at the early stage of ovarian cancer hinders the early diagnosis and contributes to the high mortality rate.1) The standard treatment of ovarian cancer is cytoreduction surgery, followed by platinum-based chemotherapy. However, most patients experience re-occurrence of cancer within 2 years of the initial treatment because of cancer cells' acquisition of resistance against platinum-based chemotherapy. A 5-year survival rate of the advanced ovarian cancer patients is reported to be only about 30%, 2) and the acquisition of cisplatin resistance is recognized as a major obstacle against the successful treatment of ovarian cancer. There are still many unresolved questions about how cancer cells become desensitized to chemotherapy.Recent advances in biomechanical studies suggest that the cellular mechanical compliance plays a crucial role in tumorigenesis and cancer progression. [3][4][5] In recent years, atomic force microscopy (AFM) is finding more attention in the field of biology and pharmaceutics.6-8) Especially, AFM-based nano-mechanics is recognized as a useful tool for detecting mechanical responses from local nanometer-sized domains of a cell.9-11) Changes in mechanical properties during pathological progressions have been investigated using AFM, 12,13) and abnorm...

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“…However, the opposite correlation-the reinforced mechanical stiffness for motile phenotypes-was observed from prostate and ovarian cancer cells. [30][31][32]43) The elastic moduli of these cancer cells lie in the lower spectrum (< 500 Pa) compared to others and thus the further decrease in the elastic moduli may not be optimal for the effective invasion. This two different correlations could be associated with differences in mechanisms governing the cellular motility-a bleb-associated migration through actomyosin contractility vs. an elongated migration through actin polymerization.…”

Section: Discussionmentioning

confidence: 98%

“…[26][27][28][29] On the contrary, the opposite correlation has been reported specially for prostate cancer cells. [30][31][32] Since the mechanical stiffness was suggested as a diagnostic marker for oral cancer cells, 33) several studies examined its possible use for prognosis. 34,35) A recent study using AFM reported the reduction of mechanical stiffness in metastatic tongue squamous cells due to changes in actin filaments and microtubules.…”

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confidence: 99%

Nano-biomechanical Validation of Epithelial–Mesenchymal Transition in Oral Squamous Cell Carcinomas

Park

Jang

Jeong

2016

Biological & Pharmaceutical Bulletin

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The effective cure for oral squamous cell carcinoma (OSCC) patients is challenging due late diagnosis and fatal metastasis. The standard diagnosis for OSCC often depends on the subjective interpretation of conventional histopathology. Additionally, there is no standard way for OSCC prognosis. Over the past decade, nano-mechanical stiffness has been considered as a quantitative measure for cancer diagnosis. Nevertheless, its application to OSCC diagnosis and prognosis is still in a primitive stage. In this study, we investigated whether the OSCC progression can be predicted by nano-mechanical properties in combination with biochemical properties, especially the epithelial-mesenchymal transition (EMT). Atomic force microscopy-based nano-mechanical measurements of three different OSCC cell lines-SCC-4, SCC-9, and SCC-15-were conducted together with biochemical analyses. The gradual upregulation of Snail2, N-cadherin, and vimentin and the simultaneous downregulation of E-cadherin were observed, and the degree of upregulation and downregulation was stronger in the order of the cell lines mentioned above. The strength of enhancement in migration was in the same order as well. Consistently, nano-mechanical stiffness was gradually decreased as the EMT progresses. These results suggest that the nano-mechanical assay could serve as a quantitative tool to predict the OSCC progression in the context of the EMT. Furthermore, we found that the upregulated vimentin, a major filamentous component of the cytoskeleton, may contribute to mechanical softening, which can be discerned from the role of actin filaments in mechanical stiffness. In conclusion, our combinational study proposes a novel way to elucidate the mechanism of OSCC progression and its therapeutic targets.Key words nano-mechanics; epithelial-mesenchymal transition; atomic force microscopy; oral cancer Oral squamous cell carcinoma (OSCC) is the most common neoplasm of the head and neck squamous cell carcinoma (HNSCC), which represents approximately 6% of all types of cancer. Also about half a million new cases are diagnosed every year.1) It is a highly invasive malignant tumor characterized by a high rate of metastasis to the cervical lymph nodes.2) Diagnosis and management of OSCC have improved by combining therapies such as radical surgery, radiotherapy and neo-adjuvant chemotherapy, but the overall 5-year survival rate is still below 50% because of late diagnosis and high rate of recurrence.3,4) Thus, it is highly desirable to understand the underlying mechanism governing metastatic dissemination of OSCC for the development of anticancer therapeutics.The progression of OSCC is largely dependent on the epithelial-mesenchymal transition (EMT). 5,6) Recent studies have reported that the EMT has been strongly associated with cancer cell stemness 7,8) and chemoresistance to anti-cancer drugs, [9][10][11][12] indicating that targeting EMT could be a good strategy to overcome cancer metastasis. Notably, transforming growth factor-β1 (TGF-β1) has been known to be a prima...

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Effect of collagen I and fibronectin on the adhesion, elasticity and cytoskeletal organization of prostate cancer cells

Cited by 54 publications

References 29 publications

Changes in water chemistry can disable plankton prey defenses

Changes in water chemistry can disable plankton prey defenses

Nano-mechanical Reinforcement in Drug-Resistant Ovarian Cancer Cells

Nano-biomechanical Validation of Epithelial–Mesenchymal Transition in Oral Squamous Cell Carcinomas

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