Effect of collection time on the viability of banana pollen grains

Soares, Taliane Leila; Souza, Everton Hilo de; Sampaio, L. F. S.; Costa, Angelica Pereira de Carvalho; Silva, Sebastião de Oliveira e; Santos-Serejo, Janay Almeida dos

doi:10.5897/ajb2015.14449

Cited by 7 publications

(3 citation statements)

References 41 publications

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“…They concluded that midday hours were more appropriate for crossing. The results are also supported by Soares et al (2015), who postulated that banana pollination success should be high in the morning (0700-1000 HR) as a result of maximal pollen viability and stigma receptivity. High pollen viability in the morning could be associated with lower temperatures and/or a lower vapor pressure deficit, and some have observed that higher temperatures harm pollen viability (Ortiz 2011;Rang et al 2011;Wang et al 2004).…”

Section: Discussionsupporting

confidence: 69%

Pollen Amount and Viability in Mchare and Selected Wild (AA) Banana (Musa acuminata) Genotypes: Prospects for Breeding

Bayo,

Massawe,

Ndakidemi

et al. 2024

horts

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East African diploid cooking bananas, commonly called Mchare, are a staple crop for millions of subsistence farmers in Tanzania, particularly in the Pangani region in northern Tanzania. Several pathogens constrain Mchare production significantly and threaten food security. Sources of resistance to these pathogens have been identified; however, partial male and female sterility impedes successful resistance introgression, complicating the breeding process. Mchare cultivars are also the only known surviving representatives of a diploid banana subgroup that contributed unreduced gametes to many of the most widely grown and successful triploid dessert bananas (‘Cavendish’, ‘Gros-Michel’, ‘Silk’, and ‘Prata’). As such, they represent an essential intermediate step in the conventional improvement of bananas worldwide. We assess the amount and viability of pollen among Mchare and wild genotypes to identify the most fertile Mchare cultivars that can be used in conventional banana improvement. Pollen was collected from 14 banana genotypes for quantification and viability testing over 7 months, and the optimal time for pollen collection was determined to be 0800 HR. Significant variation among banana genotypes in terms of both overall pollen production and percentage of pollen viability was observed. The wild-type bananas ‘Calcutta 4’ (Indian Trade Classification (ITC) 0249] and ‘Borneo’ (ITC0253) had the greatest overall pollen production (> 31,000 pollen grains/anther) and viability (∼74%), whereas ‘Ijihu Inkundu’ (ITC1460; Mchare genotype) was the least productive (almost completely sterile), with an average pollen production of a few hundred grains per anther and a viability of 7%. There were significant differences among months in terms of pollen viability, with the greatest average viability observed in May, April, and February (> 51%), and the lowest average pollen viability in July (41%). Significant differences were observed among the Mchare genotypes, with ‘Huti-White’, ‘Huti green bell’ (ITC1559), and ‘Mchare Laini’ consistently producing more substantial amounts of total pollen and an overall more significant proportion of viable pollen. This information is vital to improve Mchare bananas and the global breeding of dessert bananas. The choice of Mchare banana used in improvement programs could affect fertility and the likelihood of breeding success.

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Section: Discussionsupporting

confidence: 69%

Pollen Amount and Viability in Mchare and Selected Wild (AA) Banana (Musa acuminata) Genotypes: Prospects for Breeding

Bayo,

Massawe,

Ndakidemi

et al. 2024

horts

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“…All cultivars were planted and conserved in germplasm at the Phitsanulok Agricultural Extension and Development Center, Phitsanulok Province, Thailand. At 30 days post-flowering, the male inflorescence sample of each cultivar was collected during the anthesis stage in the morning between 0800 and 0900 h, and the sample was stored on ice until used, following the procedure of Soares et al . (2015) .…”

Section: Methodsmentioning

confidence: 99%

Pollen viabilities and gene expression profiles across Musa genomes

Mingmanit,

Boonsrangsom,

Sujipuli

et al. 2023

AoB PLANTS

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Banana (Musa spp.) is a major global economic fruit crop. However, cross-pollination from other Musa cultivars grown in nearby plantations results in seeded fruit that exceeds market demand. This study investigated pollen viability and germination and examined the expression profiles of pollen development-related genes across seven Musa genomes (AA, BB, AAA, BBB, AAB, ABB and ABBB). Twenty-three Musa cultivars were assessed for pollen viability using LAO and TTC staining methods. Results revealed that pollen viability obtained from both methods was significantly different among all the studied cultivars. Cultivars carrying BB (diploid) genomes had higher viability percentages than AA (diploid), AAA, BBB, AAB and ABB (triploid) and ABBB (tetraploid) genomes. Germination of the studied cultivars was also investigated on pollen culture medium, with results showing significant differences between the pollen of each cultivar. The best germinating cultivar was TKM (11.0%), carrying BB genome. Expression profiles of pollen development-related genes by RT-qPCR indicated that both TPD1A and MYB80 genes were highly expressed in triploid Musa genomes but the PTC1 gene showed down-regulated expression, resulting in non-viable pollen. Pollen viability, pollen germination and pollen development-related genes differed across Musa cultivars. This knowledge will be useful for the selection of male parents for Musa cross-breeding programs. Pollen viability should also be considered when planning Musa production to avoid seeded fruit.

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“…Pollen viability tests were carried out using 1% 2,3,5-triphenyltetrazolium chloride (TTC) stain diluted in Tris buffer (hydrochloric acid 0.15 M, pH 7.8) as described by Soares et al (2015). Male flowers were collected from each genotype at anthesis (7.30–10.30 am) and immediately conveyed to the laboratory for analysis.…”

Section: Methodsmentioning

confidence: 99%

Effects of In Vitro Polyploidization on Agronomic Characteristics and Fruit Carotenoid Content; Implications for Banana Genetic Improvement

et al. 2019

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Bananas (Musa spp.), native to South East Asia, have spread worldwide and are integrated into the diets of millions of people in tropical regions. Carotenoid content varies dramatically between different banana genotypes, providing an opportunity for vitamin A biofortification. Polyploidization is a useful tool for crop improvement with potential to generate new diversity, especially in a polyploid crop like bananas. Ten induced tetraploids generated from six diploid banana genotypes were evaluated for their agronomic attributes and fruit carotenoid content in comparison to their diploid progenitors. Tetraploids had distinct plant morphology, but generally displayed inferior vegetative and yield characteristics with 20% lower bunch weights than their original diploids. Similarly, a 50% decrease in fruit provitamin A carotenoids (α-carotene, 13-cis β-carotene, 9-cis β-carotene, trans-β-carotene) accompanied by a corresponding increase in lutein was recorded in induced tetraploids in comparison to their original diploids. Additionally, all lines were subjected to pollen viability tests to assess their fertility. Pollen viability tests indicated over 70% viability for induced tetraploids and diploid controls, suggesting their possible use in crosses. These findings provide a basis for the application of induced polyploidization in bananas to generate useful genetic material for integration in hybridization programmes aiming to produce vitamin A enriched triploids valuable to malnourished populations.

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Effect of collection time on the viability of banana pollen grains

Cited by 7 publications

References 41 publications

Pollen Amount and Viability in Mchare and Selected Wild (AA) Banana (Musa acuminata) Genotypes: Prospects for Breeding

Pollen Amount and Viability in Mchare and Selected Wild (AA) Banana (Musa acuminata) Genotypes: Prospects for Breeding

Pollen viabilities and gene expression profiles across Musa genomes

Effects of In Vitro Polyploidization on Agronomic Characteristics and Fruit Carotenoid Content; Implications for Banana Genetic Improvement

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