A total of 105 2-day-old quails (DOQ), reared at high temperature (390C) until 35 days of age, to investigate the effect of chitosan irradiated with early glutathione induction on ileal villus growth in quails. The research used a Completely Randomized Design (CRD) with experimental method, consist of 5 treatments with 3 replications. Each replication consist of 7 DOQs. Treatment was given from day 4 to 35, namely P0 = basal diet without irradiated chitosan and glutathione, P1 = basal diet with 100 ppm irradiated chitosan, P2 = basal diet with 450 ppm irradiated chitosan, P3 = basal diet with 100 ppm irradiated chitosan + 150 ppm glutathione, P4 = basal diet with 450 ppm irradiated chitosan + 150 ppm glutathione. Sample was collected by choosing randomly one quail in each experimental unit so there are 15 samples. Parameters observed in this research were ileal morphometrics (ileal length, number, height, and width of ileal villi) and ileal villi cell profiles (number of goblet cells, normal cells, apoptotic cells, and necrotic cells). The results showed that irradiated chitosan with glutathione induction had a significant effect (P<0,05) on morphometric and cell profile of ileal villi, except ileal length. The growth of villi ileum in quail optimal through 100 ppm irradiated chitosan with 150 ppm glutathione.