Effect of Combination Therapy of Garcinia kola Seed and Vitamin E in Acetaminophen Induced Hepatotoxicity and Oxidative Stress in Albino Rats

Abstract

Acetaminophen, used for antipyretic and analgesic purposes has been known to exhibit toxic effects on the organs because of its ability to generate free radicals, causing varieties of diseases. This study investigated the impact of the combined formed of Garcinia kola seed and Vitamin E pretreatment exposure on hepatotoxicity and oxidative stress induced by acetaminophen in Albino Rats. Five groups of animals were used for this study. Group 1 as the control received distilled water orally only, group 2 as toxicity control intoxicated with 800 mg acetaminophen intraperitoneally. The other three groups were pretreated with various doses of either Garcinia kola seed extract or vitamin E or a combined form respectively by oral gavage method for 7 days before induction with acetaminophen intraperitoneally on the 8th day and sacrificed under chloroform anaethesia. Acetaminophen induction significantly rise (p<0.05) the hepatic enzyme levels (ALT, AST, and ALP) and a marked reduction of the antioxidant enzymes (SOD, CAT and GPX) in group 2 animals when compared with the control. There was also a significant rise (p<0.05) in the MDA levels. Meanwhile the combined form of Garcinia kola seed extract and Vitamin E pretreatment exposure on the organs showed no synergetic ameliorative potentials as compared with the single pretreatment exposure with Garcinia kola and Vitamin E respectively. The morphology of the tissue cells pretreated with these combined formed exhibited features showing signs of cell damages and slow recovery from the toxicity. Therefore combining Garcinia kola and Vitamin E may lost its ameliorative and protective effect as seen in this induced acetaminophen albino rats implying that Garcinia kola seed and Vitamin E should not be taken in a combined form.

Evaluating the Nephroprotective and Hepato-protective Effects of Hypoestes rosea in Acetaminophen-Induced Toxicity in Albino Rats

“…The observed elevation of TB. CB, ALT, AST, ALP, GGT, 5'NT and LDH due to acetaminophen toxicity and challenge agree with the findings of (23)(24) in which, respectively, hepatoprotective effects of ajoene from garlic, leaf extract of Wedelia calendulacea and Garcinia kola seed with Vitamin E. against acetaminophen-induced hepatic damage were found. The observed dose-dependent reversal of acetaminophen-induced alterations in the liver enzymes and bilirubin levels by pre-administration and post-administration of aqueous extract of Hypoestes rosea suggests that this plant is hepatoprotective.…”

The Prophylactic Antioxidant and Hepatoprotective Potential of Garcinia Kola Pretreatment in Acetaminophen Induced Toxicity in Albino Rats

“…Garcinia kola could have caused the increased activities of these enzymes due to the fact that it was acting as a free radical scavenger that might destroy hepatocyte membrane integrity via oxidative damage and modulator of antioxidant enzymes. These results are in accordance with findings reported in other studies [44][45][46][47][48][49] .…”

Effect of Omega-3 Supplement on Oxidative Stress Markers in Brain and Heart Tissue Homogenates of Shock Induced Albino Rats

“…Oxidative stress is known to be the chief factor in the pathogenesis of lifestyle related diseases such as atherosclerosis, heart failure, hypertension, diabetes mellitus and malignancies. Oxidative stress is caused by accumulation of reactive oxygen species (ROS) produced as the usual by-products of cellular metabolism and from the exposure to some environmental pollutant [7]. In fact, accumulation of reactive oxygen species beyond immediate needs of the cell is said to be detrimental to the cellular structure and functional integrity resulting to oxidative degradation of critical molecules such as DNA, proteins and lipids leading to the loss of controlled by the appropriate antioxidant scavenger at the cellular levels [5.7].…”