2011
DOI: 10.1111/j.1439-0523.2009.01754.x
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Effect of combined cold pretreatment and heat shock on microspore cultures in broccoli

Abstract: Applied stress is often used to induce embryogenesis in microspore culture. Cold pretreatment has been used in cereal crops, Brassica napus and Brassica rapa microspore cultures, but seldom attempted in Brassica oleracea microspore culture. In this study, the effect of cold pretreatment (4°C) on embryogenesis in microspore culture and ploidy of regenerants resulting from those cultures was investigated in three broccoli cultivars ÔGreen EliteÕ, ÔGreen StarÕ and ÔTI-111Õ. Cold pretreatment for 1, 2 or 3 days wa… Show more

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Cited by 36 publications
(21 citation statements)
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“…In Brassica species, heat shock has been one of the most important factors to trigger microspore embryogenesis (Fan et al, 1988;Custers et al, 1994;Gu et al, 2003;Yuan et al, 2011). Right temperature and suitable duration can significantly improve the potential of microspore embryogenesis (Wang et al, 2009;Ferrie and Caswell, 2011).…”
Section: Discussionmentioning
confidence: 99%
“…In Brassica species, heat shock has been one of the most important factors to trigger microspore embryogenesis (Fan et al, 1988;Custers et al, 1994;Gu et al, 2003;Yuan et al, 2011). Right temperature and suitable duration can significantly improve the potential of microspore embryogenesis (Wang et al, 2009;Ferrie and Caswell, 2011).…”
Section: Discussionmentioning
confidence: 99%
“…Currently, the method of heat shock is commonly used for androgenic cell culture in Brassica oleracea (Takahata and Keller, 1991;Duijs et al, 1992;Fabijanski et al, 1991). Furthermore, the combination of cold pretreatment and heat shock significantly enhances microspore embryogenesis efficiency and results in higher diploid frequency of the regenerated population compared to traditional microspore culture protocol which typically uses heat shock in broccoli (Yuan et al, 2011).…”
Section: Discussionmentioning
confidence: 99%
“…Despite these improvements, anther and isolated microspore culture methods have some limitations in broccoli. One big problem is that the yield of embryo in some broccoli genotypes is still very low, and certain genotypes do not appear amenable to the process at all (Dias, 2001;Yuan et al, 2011). Meanwhile, a general problem encountered in the use of anther culture with B. oleracea is that resulting populations contain a mixture of variable ploidy individuals (Chauvin et al, 1993).…”
Section: Introductionmentioning
confidence: 99%
“…Recently, such pretreatments for 1-2 days and for 10 days were reported to enhance embryo yields in B. oleracea and B. rapa , respectively (Yuan et al 2011 ;Takahashi et al 2012 ). In addition to positive effects, the storage of buds at a low temperature could avoid the concentration of works in limited time (Sato et al 2002 ).…”
Section: Stress and Pretreatmentmentioning
confidence: 97%