Effect of Combined Exercise Versus Aerobic-Only Training on Skeletal Muscle Lipid Metabolism in a Rodent Model of Type 1 Diabetes

Dotzert, Michelle; McDonald, Matthew W.; Murray, Michael; Nickels, John Z; Noble, Earl G.; Melling, C.W. James

doi:10.1016/j.jcjd.2017.09.013

Cited by 13 publications

(20 citation statements)

References 52 publications

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“…The results obtained for plasma glucose of STZdiabetic rats showed high level of glucose as compared to the normal non-diabetic rats (Table 4). This result is in accordance with the findings of several authors using STZ-diabetic rats (Dotzert, McDonald, Murray, Noble, & Melling, 2017). Deshmukh and Jain (2015) report shows that hyperglycaemia could arise from either a defect in insulin secretion as in the case of insulindependent diabetes mellitus (type I) or a defect in insulin resistance (receptor or post receptor defect) as in the case of non-insulin-dependent diabetes mellitus (type 2) (Deshmukh & Jain, 2015).…”

Section: Discussionsupporting

confidence: 91%

Biochemical changes in diabetic rats treated with ethanolic extract of Chysophyllum albidum fruit-skin

Asagba

Kadiri

Ezedom

2019

JoBAZ

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Background: Diabetes mellitus (DM), also known as diabetes, involves a group of metabolic disorders in which blood sugar levels are high over a prolonged period. The present study was designed to evaluate the effect of 21 days administration of 100 and 200 mg per kg bodyweight of ethanolic extract of Chrysophyllum albidum (African star apple) fruit-skin on streptozotocin (STZ)-diabetic Wistar albino rats. A randomized control trial was used. Diabetic state of test rats was induced with streptozotocin at a dose of 60 mg streptozotocin/kg body using saline (0.9% w/v NaCl) as vehicle. Upon confirmation of diabetic state, crude ethanolic extract of C. albidum was dissolved in citrate buffer at pH 4.2 before administration to the rats at specific doses. Results: Results show a significant (p < 0.05) decreased in the level of plasma creatinine and insulin but increased level of glucose in diabetic rats relative to control. Induction of diabetes significantly increased the activities of alanine aminotransferases and aspartate aminotransferases in the liver and kidney and activities of both enzymes were also correspondingly increased in the plasma. There was significant increase in the level of total cholesterol, low-density lipoprotein-cholesterol, very low-density lipoprotein-cholesterol and triglyceride, but decreased highdensity lipoprotein-cholesterol in the test rats. Results also show alterations in the antioxidant status of experimental rats. The diabetic-induced changes were reversed in diabetic rats upon administration of C. albidum extract at a dose of 200 mg/kg body weight to levels comparable with control. Conclusion: The findings obtained revealed that ethanolic extract of C. albidum fruit-skin at a dose of 200 mg/kg body weight reversed the effect of diabetes due to its antihyperglycemic, anti-hyperlipidemic and antioxidant properties. The antidiabetic effect of the extract may be due to its abundant flavonoid and alkaloid contents.

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Section: Discussionsupporting

confidence: 91%

Biochemical changes in diabetic rats treated with ethanolic extract of Chysophyllum albidum fruit-skin

Asagba

Kadiri

Ezedom

2019

JoBAZ

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“…These findings would support a mechanism by which the DIT animals demonstrated a significant reduction in IMCL stores in comparison to DARE and DCT. Chronically hyperglycemic animals have been shown to demonstrate increased myocellular transmembrane lipid transporter cluster of differentiation 36 (CD36) [ 22 , 36 ], accompanied by changes in serum non-esterified fatty acids levels [ 36 ] and decreased mitochondrial citrate synthase [ 22 ] and β-oxidation activity [ 36 ]. Indeed, it has been shown that increases in CD36 in hyperglycemic animals leads to elevations in fat transmembrane transport and accumulation in skeletal muscle [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

“…One promising theory underlying the development of “double diabetes” in T1DM is the muscle-lipotoxicity theory of IR [ 7 , 13 , 21 , 22 ]. This theory posits that the improper storage of intramyocellular lipid (IMCL) metabolites in skeletal muscle leads to disruption of the insulin receptor signalling pathway.…”

Section: Introductionmentioning

confidence: 99%

“…The majority of studies examining exercise training in T1DM have focused primarily on improving indices of glycemic control, and it has been discovered that a combination of resistance and aerobic exercise provides the greatest metabolic benefits while reducing the incidence of post-exercise hypoglycemia, which is a primary concern when exercising with T1DM [ 10 , 37 – 44 ]. There is also evidence that the training adaptations from resistance and aerobic exercise may complement each other, leading to improved metabolic markers when compared to resistance or aerobic exercise alone [ 22 , 37 , 38 , 45 ]. Citrate synthase activity and muscle IMCL content, for example, were shown to increase in a combined exercise-trained T1DM muscle when compared to aerobic exercise alone, suggesting that combined training may also improve the muscle’s ability to effectively store and oxidize IMCL [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

“…There is also evidence that the training adaptations from resistance and aerobic exercise may complement each other, leading to improved metabolic markers when compared to resistance or aerobic exercise alone [ 22 , 37 , 38 , 45 ]. Citrate synthase activity and muscle IMCL content, for example, were shown to increase in a combined exercise-trained T1DM muscle when compared to aerobic exercise alone, suggesting that combined training may also improve the muscle’s ability to effectively store and oxidize IMCL [ 22 ]. Moreover, these improvements are evident in patients with T1DM who altered their insulin regimen and intentionally elevated BG to levels that represent the more traditional CIT strategy in order to mitigate hypoglycemia onset [ 10 , 37 , 39 , 46 – 49 ].…”

Section: Introductionmentioning

confidence: 99%

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The effects of exercise training versus intensive insulin treatment on skeletal muscle fibre content in type 1 diabetes mellitus rodents

2021

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Background Intensive-insulin treatment (IIT) strategy for patients with type 1 diabetes mellitus (T1DM) has been associated with sedentary behaviour and the development of insulin resistance. Exercising patients with T1DM often utilize a conventional insulin treatment (CIT) strategy leading to increased insulin sensitivity through improved intramyocellular lipid (IMCL) content. It is unclear how these exercise-related metabolic adaptations in response to exercise training relate to individual fibre-type transitions, and whether these alterations are evident between different insulin strategies (CIT vs. IIT). Purpose: This study examined glycogen and fat content in skeletal muscle fibres of diabetic rats following exercise-training. Methods Male Sprague-Dawley rats were divided into four groups: Control-Sedentary, CIT- and IIT-treated diabetic sedentary, and CIT-exercised trained (aerobic/resistance; DARE). After 12 weeks, muscle-fibre lipids and glycogen were compared through immunohistochemical analysis. Results The primary findings were that both IIT and DARE led to significant increases in type I fibres when compared to CIT, while DARE led to significantly increased lipid content in type I fibres compared to IIT. Conclusions These findings indicate that alterations in lipid content with insulin treatment and DARE are primarily evident in type I fibres, suggesting that muscle lipotoxicity in type 1 diabetes is muscle fibre-type dependant.

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Mechanisms of insulin resistance in type 1 diabetes mellitus: A case of glucolipotoxicity in skeletal muscle

Sammut,

Dotzert,

Melling

2024

Journal Cellular Physiology

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Insulin resistance (IR), a hallmark of type 2 diabetes mellitus, develops in a significant number of patients with type 1 diabetes mellitus (T1DM) despite the use of insulin therapy to control glycemia. However, little is currently understood regarding the underlying mechanisms of IR in T1DM, especially within the context of chronic insulin treatment. Recent evidence suggests an important influence of glucolipotoxicity in skeletal muscle on insulin sensitivity in T1DM. Thus, this review summarizes our current knowledge regarding impairments in skeletal muscle lipid, glucose, and oxidative metabolism in the development of IR in insulin‐treated T1DM.

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Effect of Combined Exercise Versus Aerobic-Only Training on Skeletal Muscle Lipid Metabolism in a Rodent Model of Type 1 Diabetes

Cited by 13 publications

References 52 publications

Biochemical changes in diabetic rats treated with ethanolic extract of Chysophyllum albidum fruit-skin

Biochemical changes in diabetic rats treated with ethanolic extract of Chysophyllum albidum fruit-skin

The effects of exercise training versus intensive insulin treatment on skeletal muscle fibre content in type 1 diabetes mellitus rodents

Mechanisms of insulin resistance in type 1 diabetes mellitus: A case of glucolipotoxicity in skeletal muscle

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