Effect of complement component 5 polymorphisms on mastitis resistance in Egyptian buffalo and cattle

El-Halawany, Nermin; Shawky, Abd-El-Monsif A.; Al-Tohamy, Ahmed F.M.; Abdel-Latif, Magdy A.; Abdel-Shafy, Hamdy; Ghazi, Yasser A.; Neuhoff, Christiane; Schellander, K.

doi:10.1016/j.rvsc.2018.05.015

Cited by 7 publications

(2 citation statements)

References 41 publications

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“…Whether water buffalo susceptibility to LSD infection could be ascribed to specific genetic variants [ 38 ], as for tuberculosis, mastitis, or foot-and-mouth disease [ 39 , 40 , 41 , 42 ], is difficult to say but the hypothesis deserves to be further explained in future studies.…”

Section: Discussionmentioning

confidence: 99%

Susceptibility of Mediterranean Buffalo (Bubalus bubalis) following Experimental Infection with Lumpy Skin Disease Virus

Di Felice,

Pinoni,

Rossi

et al. 2024

Viruses

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Lumpy skin disease (LSD) is a viral disease of cattle and water buffalo characterized by cutaneous nodules, biphasic fever, and lymphadenitis. LSD is endemic in Africa and the Middle East but has spread to different Asian countries in recent years. The disease is well characterized in cattle while little is known about the disease in buffaloes in which no experimental studies have been conducted. Six buffaloes and two cattle were inoculated with an Albanian LSD virus (LSDV) field strain and clinically monitored for 42 days. Only two buffaloes showed fever, skin nodules, and lymphadenitis. All samples collected (blood, swabs, biopsies, and organs) were tested in real-time PCR and were negative. Between day 39 and day 42 after inoculation, anti-LSDV antibodies were detected in three buffaloes by ELISA, but all sera were negative by virus neutralization test (VNT). Cattle showed severe clinical signs, viremia, virus shedding proven by positive real-time PCR results, and seroconversion confirmed by both ELISA and VNT. Clinical findings suggest that susceptibility in buffaloes is limited compared to in cattle once experimentally infected with LSDV. Virological results support the hypothesis of buffalo resistance to LSD and its role as an accidental non-adapted host. This study highlights that the sensitivity of ELISA and VNT may differ between animal species and further studies are needed to investigate the epidemiological role of water buffalo.

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Section: Discussionmentioning

confidence: 99%

Susceptibility of Mediterranean Buffalo (Bubalus bubalis) following Experimental Infection with Lumpy Skin Disease Virus

Di Felice,

Pinoni,

Rossi

et al. 2024

Viruses

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“…This high conservation agrees with earlier mapping studies of water buffalo depicting syntenic block coverage of >80% (62). Many mastitis associated genes like complement C5 (C5) and chemokine (C-X-C motif) receptor 1 (CXCR1), are reported to be conserved as high as up to 99% sequence similarity (63,64). This extensive conservation of cattle genes in water buffalo is due to their common ancestor, homologous proteins, common gene family, common CNV, common gene statistics, across species selection signature of milk pathways are also revealed in buffalo genome Benchmarking Universal Single-Copy Orthologs (BUSCO) mapping studies (62).…”

Section: Mining and Mapping Of Bovine Mastitis Associated Genesmentioning

confidence: 99%

Genome Wide Prediction, Mapping and Development of Genomic Resources of Mastitis Associated Genes in Water Buffalo

et al. 2021

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Water buffalo (Bubalus bubalis) are an important animal resource that contributes milk, meat, leather, dairy products, and power for plowing and transport. However, mastitis, a bacterial disease affecting milk production and reproduction efficiency, is most prevalent in populations having intensive selection for higher milk yield, especially where the inbreeding level is also high. Climate change and poor hygiene management practices further complicate the issue. The management of this disease faces major challenges, like antibiotic resistance, maximum residue level, horizontal gene transfer, and limited success in resistance breeding. Bovine mastitis genome wide association studies have had limited success due to breed differences, sample sizes, and minor allele frequency, lowering the power to detect the diseases associated with SNPs. In this work, we focused on the application of targeted gene panels (TGPs) in screening for candidate gene association analysis, and how this approach overcomes the limitation of genome wide association studies. This work will facilitate the targeted sequencing of buffalo genomic regions with high depth coverage required to mine the extremely rare variants potentially associated with buffalo mastitis. Although the whole genome assembly of water buffalo is available, neither mastitis genes are predicted nor TGP in the form of web-genomic resources are available for future variant mining and association studies. Out of the 129 mastitis associated genes of cattle, 101 were completely mapped on the buffalo genome to make TGP. This further helped in identifying rare variants in water buffalo. Eighty-five genes were validated in the buffalo gene expression atlas, with the RNA-Seq data of 50 tissues. The functions of 97 genes were predicted, revealing 225 pathways. The mastitis proteins were used for protein-protein interaction network analysis to obtain additional cross-talking proteins. A total of 1,306 SNPs and 152 indels were identified from 101 genes. Water Buffalo-MSTdb was developed with 3-tier architecture to retrieve mastitis associated genes having genomic coordinates with chromosomal details for TGP sequencing for mining of minor alleles for further association studies. Lastly, a web-genomic resource was made available to mine variants of targeted gene panels in buffalo for mastitis resistance breeding in an endeavor to ensure improved productivity and the reproductive efficiency of water buffalo.

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Molecular Evolution and Genome Architecture of Water Buffalo (Bubalus Bubalis), the “Living Bank” for Marginal Farmers in Developing Countries

Mukherjee

2022

Biotechnological Applications in Buffalo Research

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Effect of complement component 5 polymorphisms on mastitis resistance in Egyptian buffalo and cattle

Cited by 7 publications

References 41 publications

Susceptibility of Mediterranean Buffalo (Bubalus bubalis) following Experimental Infection with Lumpy Skin Disease Virus

Susceptibility of Mediterranean Buffalo (Bubalus bubalis) following Experimental Infection with Lumpy Skin Disease Virus

Genome Wide Prediction, Mapping and Development of Genomic Resources of Mastitis Associated Genes in Water Buffalo

Molecular Evolution and Genome Architecture of Water Buffalo (Bubalus Bubalis), the “Living Bank” for Marginal Farmers in Developing Countries

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