Effect of Complexes of Cobalt With Aminoacids on the Replicationof Herpes Simplex Virus Type 1 (HSV‐1)

Abstract

Cobalt, being essential metal, influences different physiological and enzymatic functions. As cobalt does not accumulate in the body, Co-compounds have relatively low toxicity. The aim of the present study is the effect of complexes of Co(II) with aminoacids - lysine, arginine, histidine and serine on HSV-1 replication. No effect of [O2Co(his)4].nH2O and [O2Co(arg)2].nH2O on HSV-1 infection in vitro was found. Both, [O2Co(lys)2].nH2O and [O2Co(ser)2].nH2O suppress the attachement of HSV-1 particles onto target cells and the viral replication as well. Moreover, the properties of the particular Co-complex (charge, stability, structure) are manifestated by their virucidal effect. Thus, [O2Co(ser)2].nH2O irreversibly inhibits the infectious activity of free HSV-1 virions, while virucidal effect of [O2Co(lys)2].nH2O is completely reversible after the 2h of contact.

Effect of Copper Acyclovir Complexes on Herpes Simplex Virus Type 1and Type 2 (HSV‐1, HSV‐2) Infection in Cultured Cells

“…We have found that when copper, zinc or cobalt is bound to a suitable ligand, the appropriate complex exibited a significant anti-HSV effect (Varadinova et al, 1993;1996). Recently published data by Sagripanti et al (1997) also show that the inhibition of HSV by copper was enhanced by reducing agents and that mechanism of the inactivation is similar as for coppermediated DNA damage (Aruoma, et al 1991;Dizdaroglu, et al, 1991;Toyokuni and Sagripanti, 1994).…”

“…As some of the complexes tested significantly inhibited HSV growth (Varadinova et al, 1996; Sagripanti et al, 1997), we venture to propose that it is possible to increase the anti-HSV activity of ACV by complexing Cu(ll), with special attention on the action towards replication of ACV-resistant mutant. For this purpose we used three coordination compounds in which the roles of Cu(ll) and ACV are distinctively different-[Cu(ACV)2CI2(H20)2]; Cu(ACV)2(H20)3](NO3)2.H20 and [Cu(ACV)2(H20)2](NO3)2].…”