Eighteen Simmental heifers were fed concentrate and barley straw offered as a total mixed ration (TMR) or separately as a free choice (FCH) to compare performance, behaviour, and meat quality. The heifers were assigned to treatments in a randomized complete block design. Animals were allotted to roofed pens with 3 animals per pen, and 3 pens per treatment. Intake of concentrate, average daily gain, and gain to feed ratio were not different between diets, being on average 7.6 kg/day, 1.38 kg/day and 0.18 kg/kg, respectively. Straw intake was greater in TMR than in FCH treatment (0.7 vs 0.3 kg/day, respectively; p<0.001). Crude protein intake, neutral detergent fibre intake and water consumption did not differ between treatments. Time spent eating was longer in FCH than in TMR (p=0.001), whereas time spent ruminating and total chewing time were longer (p<0.01) in TMR than in FCH. The number of displacements resulting from competition for feed in the main feeder in TMR treatment tended to be greater than in FCH treatment. There were no differences in the carcass characteristics and quality of meat of animals assigned to the different feeding methods, but the percentage of 18:2 n-6 was higher in FCH treatment. In summary, these results suggest that the use of TMR as a feeding method in beef cattle fed high concentrate diets did not affect performance and increased time spent ruminating with a potential decrease of ruminal acidosis incidence.Additional key words: beef cattle; feeding method; high concentrate diets. Abbreviations used: ADF (acid detergent fibre); ADG (average daily gain); BW (body weight); CLA (conjugated linoleic acid); CP (crude protein); DM (dry matter); FAME (fatty acid methyl esters); FCH (free choice diet); G:F (gain to feed ratio); LT (Longissimus thoracis muscle); MUFA (monounsaturated fatty acids); n-3 (omega 3 fatty acids); n-6 (omega 6 fatty acids); NDF (neutral detergent fibre); NFC (non-fibre carbohydrates); PUFA (polyunsaturated fatty acids); SFA (saturated fatty acids); TMR (total mixed ration); WBSF (Warner-Bratzler shear force).Citation: Iraira, S. P.; Madruga, A.; Pérez-Juan, M.; Ruíz-de-la-Torre, J. L.; Rodriguez-Prado, M.; Calsamiglia, S.; Manteca, X.; Ferret, A. (2015). Performance, behaviour and meat quality of beef heifers fed concentrate and straw offered as total mixed ration or free-choice. Spanish Journal of Agricultural Research, Volume 13, Issue 4, e0610, 11 pages. http://dx