Effect of concentrated growth factor (CGF) on postoperative sequel of completely impacted lower third molar extraction: a randomized controlled clinical study

Elayah, Sadam Ahmed; Xiang, Lei; Sakran, Karim Ahmed; Xie, Linyang; Younis, Hamza; Alajami, Ahmed Es.; Tu, Jiasheng; Na, Sijia

doi:10.1186/s12903-022-02408-7

Cited by 22 publications

(25 citation statements)

References 39 publications

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“…Fang et al [22] showed that the lling of CGF gel in tooth extraction can signi cantly reduce the pain response of patients 2h, 24h and 48h after surgery. Elayah et al [23] also showed that CGF could signi cantly reduce the pain response caused by tooth extraction at 3 and 7 days after surgery. This may be related to the following aspects that CGF is a brous protein structure which acts as a scaffold material and reservoir to transfer growth factors and help accelerate the healing stage [24].…”

Section: Discussionmentioning

confidence: 96%

A novel concentrated growth factor (CGF)-based strategy for second molar protection after impacted mandibular third molar extraction: a randomized controlled clinical study

Sun

Zhang

et al. 2023

Preprint

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Background：The extraction of impacted mandibular third molars might cause large bone defects in the distal area of second molars. A new strategy was innovatively employed here combining autologous bone, Bio-Oss, concentrated growth factors (CGF) gel and CGF membrane for bone repair, and the present study aimed at exploring safety as well as short- and long-term efficacy of this new protocol clinically. Materials and methods: A total of 66 participants were enrolled in this randomized single-blind clinical trial, and randomly allocated to control group (only blood clots), test A group (autogenous bone，Bio-Oss with oral repair membrane) and test B group (autogenous bone，Bio-Oss, CGF gel with CGF membrane). The postoperative outcomes including PoSSe scale, periodontal probing depth (PD), degree of gingival recession and computed tomography measurements were assessed at 3th, 6th, 12th month. A p-value<0.05 was considered statistically significant. Results: In PoSSe scale, no significant difference was observed except a significant alleviation of early-stage pain perception in test B group (p<0.05). Also, test B group exhibited better effect on periodontal healing and gingival recession reduction after 6 months (p<0.05). Both two test groups showed more new bone formation than the control group (p<0.05). It is noteworthy that the bone repair of test B group was significantly better than that of test A at 3th and 6th month (p<0.05), yet no difference was observed at 12th month (p>0.05). Conclusion: Both two test groups could achieve stable long-term efficacy on alveolar bone repair. The use of CGF gel and CGF membrane could accelerate early-stage bone repair, alleviate short-term pain after surgery, reduce long-term probing depth and relieve economic cost for patients. This new bone repair protocol is worthy of promoting by clinicians. Trial registration: This study was registered with the identification number ChiCTR2300068466 on 20/02/2023 at Chinese Clinical Trial Registry. Also, it was ethically approved from the institutional ethics committee at the Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (No:2023-010-01), and has been conducted in accordance to the guidelines of the declaration of Helsinki. Written informed consent was obtained from all participants in the study.

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Section: Discussionmentioning

confidence: 96%

A novel concentrated growth factor (CGF)-based strategy for second molar protection after impacted mandibular third molar extraction: a randomized controlled clinical study

Sun

Zhang

et al. 2023

Preprint

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“…These mixed results from clinical trials have hindered the application of growth factors. CGF, obtained from a patient's autologous venous blood, is characterized by rich growth factors and fibrin, which have concert effect in treatment thus leading to beneficial outcomes [14,35].…”

Section: Discussionmentioning

confidence: 99%

Concentrated Growth Factor Promotes Wound Healing Potential of HaCaT Cells by Activating the RAS Signaling Pathway

Liu¹,

Liu²,

Zeng³

et al. 2022

Front. Biosci. (Landmark Ed)

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Objective:The aim of this study was to explore the effect of concentrated growth factor (CGF) on the wound healing potential of human epidermal cells (HaCaT) in vitro and in vivo. Methods: CGF was extracted from venous blood using the centrifugal separation method. The CGF-conditioned medium was prepared from CGF gel immersed in Dulbecco's Modified Eagle medium. Crystal violet staining and wound healing assay were used to evaluate the proliferation and migration of HaCaT cells, respectively. Lipopolysaccharide (LPS) was used to test the anti-inflammatory function of CGF. An ELISA kit was employed to detect the concentration of growth factors and interleukins in CGF medium. mRNA and protein levels of angiogenic biomarkers (Angiopoietin-1 (ANGPT-1), vascular endothelial growth factor-A (VEGF-A) and Angiopoietin-2 (ANGPT-2) ) were determined by quantitative polymerase chain reaction (qPCR) and Western blot, respectively. A dorsal excisional wound model was recruited to test the wound healing effect of CGF in mice. Results: Three-day treatment of HaCaT cells with CGF significantly promoted cell proliferation, which was followed by an increase in Vascular Endothelial Growth Factor (VEGF) and Fibroblast Growth Factor (FGF) levels in the medium. Cytokines (IL-6, IL-8 and TNF-α) were increased in LPS-stimulated HaCaT cells after 3 days, and CGF slightly inhibited the mRNA expression of these cytokines. The RAS signaling pathway was activated upon CGF treatment. Both RAS knockdown and an inhibitor of RAS (zoledronic acid) could block the migration of HaCaT cells after CGF treatment. Protein expressions of CD31, ANGPT-1, and VEGF-A were up-regulated in a dosedependent manner upon CGF exposure. The protein level of ANGPT-2 was down-regulated after CGF treatment. CGF could promote wound healing in vivo, as demonstrated using the full skin defect model in nude mice. Conclusions: CGF was shown to promote wound repair in vitro and in vivo. The RAS cell signaling pathway was responsible for CGF stimulating the wound healing potential of HaCaT cells.

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“…CGF is obtained by centrifuging blood samples at varying speeds using a special centrifuge device (Medifuge, Silfradentsrl, Italy). This technique, with its different and controlled centrifuge speeds, allows for the production of a much larger and denser brin matrix with abundant growth factors [19][20][21]. Additionally, studies have found the presence of CD34 + stem cells, which play an important role in angiogenesis, in PRF concentrates.…”

Section: Introductionmentioning

confidence: 99%

Effect of concentrated growth factor(CGF) on postoperative patient comfort in impacted lower third molars undergoing coronectomy: a prospective clinical study

Dudak,

Calis,

Koca

2024

Preprint

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Objectives The aim of this study was to evaluate the effect of CGF on post-operative patient comfort following coronectomy in impacted third molars with roots associated with the nervus alveolaris inferior. Materials and Methods Thirty-five patients with bilateral vertical impacted wisdom teeth with roots associated with the nervus alveolaris inferior were included in the study with an indication for coronectomy. Coronectomy was performed on both sides followed by cgf on one side. Pain levels were monitored with the VAS scale for 7 post-operative days. In addition, the amount of edema occurring on post-operative days 2 and 7 was also measured. All data were statistically evaluated. Statistical significance was determined as p<0.001. Results The first 6 days showed significantly lower (p<0.001) pain on the CGF-treated side, while the difference between the sides was not significant on the 7th day (p=0.627). On the 2nd day, significantly lower (p<0.001) edema occurred in the tragus-pogonion and angulus-lateral canthus planes on the CGF treated side. On day 7, there was no significant difference in edema between the sides. Uneventful healing was observed in all patients. Conclusion It is possible to prevent possible nerve damage with coronectomy. In addition, CGF can successfully improve patient comfort in the early post-operative period. Clinical Relevance Post-operative pain and edema, which are very important for patients, can be significantly reduced with the application of CGF, an easily obtainable autogenous product. The results obtained may provide valuable contributions to clinicians.

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Effect of concentrated growth factor (CGF) on postoperative sequel of completely impacted lower third molar extraction: a randomized controlled clinical study

Cited by 22 publications

References 39 publications

A novel concentrated growth factor (CGF)-based strategy for second molar protection after impacted mandibular third molar extraction: a randomized controlled clinical study

A novel concentrated growth factor (CGF)-based strategy for second molar protection after impacted mandibular third molar extraction: a randomized controlled clinical study

Concentrated Growth Factor Promotes Wound Healing Potential of HaCaT Cells by Activating the RAS Signaling Pathway

Effect of concentrated growth factor(CGF) on postoperative patient comfort in impacted lower third molars undergoing coronectomy: a prospective clinical study

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