Effect of Concentration of Ionic Liquid 1‐Butyl‐3‐Methylimidazolium, Tetrafuoroborate, for Retention and Separation of Some Amino and Nucleic Acids

Polyakova, Yulia; Jin, Yinzhe; Zheng, Jinzhu; Row, Kyung Ho

doi:10.1080/10826070600716769

Cited by 37 publications

(24 citation statements)

References 20 publications

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“…Ionic liquids are attracting considerable attention because they are a green, high-technology reaction media showing excellent chemical properties [24,25], and have been applied to many fields of analytical chemistry [26], such as sample preparation [27], organic synthesis [28][29][30][31], liquid-phase extraction [32,33], and chromatographic separations [34].…”

Section: Introductionmentioning

confidence: 99%

Solid‐phase extraction of liquiritin and glycyrrhizic acid from licorice using ionic liquid‐based silica sorbent

Tian

Row

2009

J of Separation Science

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A new ionic liquid-based silica sorbent was developed by a process involving surface chemical modification of commercial silica using a synthesized ionic liquid. The obtained particles were successfully used as a special sorbent in a solid-phase extraction process to isolate liquiritin and glycyrrhizic acid from licorice. Different washing and elution solvents, such as water, methanol/water (v/v), and pure methanol were evaluated. Ionic liquid-based silica sorbent was compared with traditional C 18 sorbent and it exhibited higher selectivity. Quantitative analysis was carried out by using a C 18 column. Good linearities of two compounds were obtained from 5 Â 10 À4 to 0.2 mg/mL (r 2 40.99) with the relative standard deviations o1.0%. The target compounds in commercial herbal medicines containing licorice were determined, and the bound rates between the target compounds and protein were obtained by this sorbent.

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Section: Introductionmentioning

confidence: 99%

Solid‐phase extraction of liquiritin and glycyrrhizic acid from licorice using ionic liquid‐based silica sorbent

Tian

Row

2009

J of Separation Science

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“…Due to the above-mentioned unique properties, ILs are regarded as a new class of green solvents [30,35] and have been successfully applied in sample extraction [31,36,37], chromatography separation [38,39], and MS identification [40][41][42]. Anderson et al [31] applied ILs containing tris(pentafluoroethyl)trifluorophosphate anion for the extraction of polycyclic aromatic hydrocarbons.…”

Section: Introductionmentioning

confidence: 99%

“…Ionic liquids (ILs) are liquids composed completely of ions, and thus have unique physicochemical properties, including broad liquid range, low to negligible vapor pressures, high thermal stability, and high solubility for different classes of analytes [30][31][32][33][34][35][36][37][38][39][40][41][42]. Therefore, ILs environment is quite different from that of all polar or non-polar organic solvents [32], which is capable of forming virtually all possible types of interactions with solutes [33,34].…”

Section: Introductionmentioning

confidence: 99%

Ionic liquid 1-butyl-3-methyl imidazolium tetrafluoroborate for shotgun membrane proteomics

et al. 2010

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The solubility and digestion efficiency are two crucial factors that might affect the identification of integral membrane proteins (IMPs). In this work, 1% (v/v) ionic liquid (IL), 1-butyl-3-methyl imidazolium tetrafluoroborate (BMIM BF 4 ), added in NH 4 HCO 3 buffer (pH 8.3), was applied as a sample preparation buffer for IMPs analysis. Compared to the commonly used sodium dodecyl sulfate and methanol methods, the number of identified IMPs from rat brain by microcolumn reversed phase liquid chromatography (μRPLC)-electrospray ionization tandem mass spectrometry (ESI-MS/MS) was improved by over three times, which might be due to the fact that BMIM BF 4 offered high solubilizing ability for IMPs and good compatibility for tryptic digestion. Furthermore, compared to Rapigest and urea methods, with BMIM BF 4 method, the number of identified IMPs from rat brain could be improved 25% and 80%, respectively, which might be contributed to the good solubilizing ability and high thermal stability of such IL. With the sample treated by BMIM BF 4 method, by 2D-nanoSCX-RPLC-ESI-MS/MS, 1,450 non-redundant proteins and 7,978 unique peptides were identified from rat brain, and 418 proteins contained at least one predicted transmembrane domain, with false discovery rates of less than 1% for peptide identification, and at least two identified unique peptides per protein. All these results demonstrate that the BMIM BF 4 method is of high potential for the large-scale identification of IMPs.

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“…Numerous works describing successful applications of ILs in this field are reported annually. [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18] Nucleotides in particular are a subject of study in many fields of science, such as biology, chemistry, and medicine, and are known to play critical roles in many processes. 19 Many RPLC methods have been applied to the analysis of nucleotides.…”

Section: Introductionmentioning

confidence: 99%

Effect of Concentration of Ionic Liquids on Resolution of Nucleotides in Reversed-phase Liquid Chromatography

2007

Bulletin of the Korean Chemical Society

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The chromatographic behaviors of nucleotides (inosine 5'-monophosphate, uridine 5'-monophosphate, guanosine 5'-monophosphate, and thymine monophosphate disodium salts) on a C18 column were studied with different types of ionic liquids (ILs) as additives for the mobile phase in reversed-phase liquid chromatography (RPLC). Three ILs, 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIm][BF4]), 1-ethyl-3-methylimidazolium tetrafluoroborate ([EMIm][BF4]), and 1-ethyl-3-methylimidazolium methylsulfate ([EMIm][MS]), were used. Eluents were composed of water and methanol (90/10%, vol) with the addition of 0.5-13.0 mM of ILs. The effects of the concentration of ILs on retention and separation were investigated and discussed. The results showed that the addition of ILs affects the retention and resolution of the tested compounds. Use of 13.0 mM of [BMIm][BF4] as the eluent modifier resulted in a baseline separation of nucleotides without requiring gradient elution. This study demonstrates that ILs can be potentially applied as a mobile phase modifier in RPLC.

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Effect of Concentration of Ionic Liquid 1‐Butyl‐3‐Methylimidazolium, Tetrafuoroborate, for Retention and Separation of Some Amino and Nucleic Acids

Cited by 37 publications

References 20 publications

Solid‐phase extraction of liquiritin and glycyrrhizic acid from licorice using ionic liquid‐based silica sorbent

Solid‐phase extraction of liquiritin and glycyrrhizic acid from licorice using ionic liquid‐based silica sorbent

Ionic liquid 1-butyl-3-methyl imidazolium tetrafluoroborate for shotgun membrane proteomics

Effect of Concentration of Ionic Liquids on Resolution of Nucleotides in Reversed-phase Liquid Chromatography

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