Effect of Concentration of Platelet-Derived Growth Factor on Proliferative Activity of Human Fibroblasts

Макаров, М. С.; Storozheva, M. V.; Konyushko, O. I.; Borovkova, N. V.; Хватов, В. Б.

doi:10.1007/s10517-013-2199-9

Cited by 8 publications

(4 citation statements)

References 15 publications

(19 reference statements)

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“…Autofluorescence ability is the natural feature of collagen, which allows visualizing the collagen-containing components in fluorescent microscope to assess architecture and density of collagen [18]. In collagen bands autofluorescence intensity level averaged 9.5 ft-candelas, in dermal matrix-28.6 ft-candelas, in CDB-85.9 ft-candelas.…”

Section: Resultsmentioning

confidence: 99%

Use of Platelet-Rich Plasma for Collagen Matrixes Revitalization with Human Fibroblast

Макаров¹,

Borovkova²,

Konushko³

et al. 2015

JBM

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We studied in vitro attachment, proliferation and survival of сadaver skin fibroblasts in collagen bands, dermal matrix and cancellous demineralized bone, enriched with platelet components. It was shown, that PRP enhanced revitalization of collagen grafts, especially followed by components of activated or freezed platelets. Fibroblasts had the best rate of proliferative activity in response to 150 pg platelet derived growth factor (PDGF), released from 100 million platelets with granules and measured for 100 × 10 3 cultivated cells. The use of platelet-derived material could increase fibroblast proliferation activity for 1.5 -3 times in all types of collagen transplants without damage or decay of cell's biological value.

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Section: Resultsmentioning

confidence: 99%

Use of Platelet-Rich Plasma for Collagen Matrixes Revitalization with Human Fibroblast

Макаров¹,

Borovkova²,

Konushko³

et al. 2015

JBM

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“…Последующее культивирование проводили 3 сут с ежедневным контролем динамики роста клеток. Число клеток и их жизнеспособность оценивали с помощью оригинальных методов, основанных на витальном окрашивании клеток и их исследовании во флуоресцентном микроскопе [14]. Для окраски клеток использовали витальные флуорохромные красители трипафлавин -акридиновый оранжевый и трипафлавин -родамин С («Диаэм», Россия).…”

Section: материал и методыunclassified

“…Все эти процедуры так или иначе вызывают изменение нативной структуры амниона, что может сопровождаться значительной потерей репаративных факторов. Перспективным источником факторов репарации является богатая тромбоцитами плазма (БоТП), полученная из собственной крови пациента [11][12][13][14]. Одним из распространенных препаратов на основе БоТП является лизат тромбоцитов, который получают путем криодеструкции клеток БоТП.…”

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The biological effect of a combination of platelet lysate and amniotic membrane in buccal epithelium culture

Chentsova

Borovkova²,

Макаров

et al. 2022

Rossijskij oftalʹmologičeskij žurnal

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Purpose: To study the biological effect of a combination of platelet lysate and amniotic membrane, preserved by various techniques, on human buccal epithelium culture. Materials and methods. Human amnion transplants were preserved using 3 methods: silicate drying, lyophilization, cryopreservation. The blood of healthy volunteers was used as a source of platelets. Platelet-rich plasma (PRP) with a platelet content over 1000 thousand/mcl and more was isolated from the donors blood, frozen at -80 °С and defrosted at 0–4 °С to prepare platelet lysate. Growth-stimulating effect of the amnion transplants was studied in different groups: control group 1 — without amnion and without PRP lysate; control group 2 — PRP lysate without amnion; experimental group 1 — amnion without PRP lysate; experimental group 2 — amnion samples combined with PRP lysate. The study was carried out on the example of human buccal epithelium culture of 3–5 passages. The dynamics of cell growth was evaluated after 1, 2 and 3 days from the moment of seeding. The number of cells and their viability were evaluated using original methods based on vital cell staining and their examination in a fluorescent microscope. Results. All samples of preserved amnions were non-toxic and did not damage the structural and functional characteristics of the buccal epithelium. On the other hand, the use of amnion without PRP lysate did not have a growth-stimulating effect on cells. Among the amnion samples combined with PRP lysate, the combination of lyophilized amnion and PRP lysate was the most effective during the entire study period. Conclusions. Silicate drying, lyophilization and cryopreservation of the amniotic membrane makes it possible to obtain biocompatible and non-toxic transplants, based on human amnion. Lyophilized amnions are the most optimal for saturating PRP lysate. The combination of lyophilized amnion and PRP lysate stimulates cell growth in vitro without violating their structural integrity.

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“…Evaluation of graft biocompatibility with normal cells and tissues is of paramount importance, irrespective of whether the objective of complete tissue graft survival is set or not. First of all, this implies the absence of toxic effect on cultured human diploid cells [6,10,11,20]. Besides, an important property of tissue grafts is their adhesive capabilities.…”

Section: сLinical Medicinementioning

confidence: 99%

Collagen Fiber Autofluorescence Level in Evaluating the Biological Properties of Tissue Grafts

Макаров¹,

Storozheva²,

Borovkova³

2017

Sovrem Tehnol Med

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The aim of the investigation was to study the possibilities of evaluating the biological properties of various tissue grafts analyzing autofluorescence level of their collagen fibers.Materials and Methods. The study involved various types of standard-produced tissue grafts, some of them being exposed to additional treatment to stimulate changes in collagen fiber structure. Analysis of preparations was made using light, fluorescent and confocal microscopy. To evaluate human cell adhesion on tissue grafts with different autofluorescence levels, M-22 human fibroblasts and human blood platelets were used.Results. Collagen autofluorescence has been registered in both fixed and non-fixed tissue graft samples, autofluorescence level of single fibers reflecting the extent of their compaction. Damage to the initial collagen structure leads to changes in tissue graft composition due to formation of picrinophilic fibers with very high autofluorescence levels, which do not appear in normal tissues or after collagen decondensation in conditions of hypotonia. Grafts with collagen fiber autofluorescence intensity 30 foot-candles are highly adhesive for M-22 human fibroblasts and donor platelets, whereas grafts with autofluorescence levels of more than 40 foot-candles exhibit significantly lower adhesive properties.Conclusion. Collagen autofluorescence analysis provides important data about connective tissue topography, intercellular matrix integrity and allows collagen defects to be revealed, even in non-fixed tissue slices and grafts.

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Effect of Concentration of Platelet-Derived Growth Factor on Proliferative Activity of Human Fibroblasts

Cited by 8 publications

References 15 publications

Use of Platelet-Rich Plasma for Collagen Matrixes Revitalization with Human Fibroblast

Use of Platelet-Rich Plasma for Collagen Matrixes Revitalization with Human Fibroblast

The biological effect of a combination of platelet lysate and amniotic membrane in buccal epithelium culture

Collagen Fiber Autofluorescence Level in Evaluating the Biological Properties of Tissue Grafts

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