Effect of Concentration on the Microbiological Hydroxylation of Progesterone1

Abstract

10IICROBIOLOGICAL HYDROXYLATION OF PROGESTERONE These substances offer a possible solution for reducing the contamination and spoilage of packaged vegetables and thus extending storage life. REFERENCES D)OUGILAS, M. 1930 Some principles regulating life and death of pathogenic intestinal bacteria. Lancet, 2, 789-791.

Bacterial degradation of bile salts

“…Nowadays, corticoids and other steroid hormones are mainly produced via biotransfomlations of low-cost plant sterols as main substrates. An important milestone in steroid biotransformation was the discovery of II a.hydroxylation of progesterone by the mould Rhizopus (Weaver et al 1960). In the meantime, hydrocortisone production from ethanol has been accomplished in engineered cells of Saccharomyces cerevisiae (Szczebara et al 2003;Dumas et .al.…”

Microbial hydroxylation of progesterone withAcremonium strictum

“…Metabolites obtained may be pharmacologically active or can be used in the commercial synthesis of other useful steroid compounds [12,13]. Some kinds of these important biotransformations consisted in 11β‐, 16α‐, 17α‐ and 21‐hydroxylations of progesterone producing corticosteroids [14–16].…”

Adsorption of Lithocholic Acid to Fusarium equiseti M41 as an Essential Process in Its Conversion to Ursodeoxycholic Acid

“…At the indicated times, samples were withdrawn and centrifuged, and the LCA-binding activity of the recovered cell wall was measured. (B) After 20 min of sonication, the supematant was concentrated by lyophilization and 100 ,ug of protein was applied onto a 10% gel Lane a, Stained with Coomassie brilliant blue G-250; lane b, stained with fuchsine and perchloric acid (25). (C) Sample (100 ,u) was incubated in the absence and presence of 18 mM LCA for 30 min at 28°C and mixed with 1.0 ,uCi of ['4C]LCA.…”

“…There has been considerable interest recently in the biotransformation of lipophilic compounds such as aromatic hydrocarbons and steroidal compounds. In studies on aromatic hydrocarbons such as naphthalene (4-6) and halogenated phenol (8,11,14), their microbial degradation and detoxification in the environment have been examined, while in studies on steroidal compounds such as progesterone, the aim has been high productivity, and therefore, concentrations much higher than those necessary for the compounds to be soluble in water have usually been used (7,23,25). In this situation, the bulk of the steroidal substrates remained insoluble during the biotransformation, but the conversion was still efficient.…”