Effect of Conditioning on the Increase of Heart Rate and Body Temperature Provoked by Handling in the Mouse

Krämer, K.; Weerd, Heleen van de; Mulder, Anne; Heijningen, Caroline van; Baumans, V.; Remie, R; Voss, Hans–Peter; Zutphen, Bert F. M. Van

doi:10.1177/026119290403201s29

Cited by 26 publications

(19 citation statements)

References 21 publications

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“…While there is evidence that this might be the case to some degree and in some circumstances, there is also substantial evidence to the contrary -i.e. that stress may become worse due to repeat exposures; that habituation/desensitisation applies to only some types of stressor and not others; that while some indicators of stress may decrease, others do not, confounding the issue; and so on (see various examples [7][8][9][10][11][12][13] ).…”

Section: Jarrod Baileymentioning

confidence: 99%

Does the Stress Inherent to Laboratory Life and Experimentation on Animals Adversely Affect Research Data?

Bailey¹

2017

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Section: Jarrod Baileymentioning

confidence: 99%

Does the Stress Inherent to Laboratory Life and Experimentation on Animals Adversely Affect Research Data?

Bailey¹

2017

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“…Syversen et al (2008) for example found fluctuations in temperature of 11 °C during air transfer in more than 60% of shipments [ 16 ]. It is known that stress-induced-hyperthermia and ambient temperature (T a ) affect body temperature (T b ) [ 17 , 18 , 19 , 20 , 21 , 22 ]. Results of studies measuring T b before and after transfer, however, are inconsistent: Capdevilla et al (2007) [ 11 ] found no effect of transfer of rats on T b , while Dallman et al (2006) [ 23 ] observed an increase in T b of rats when moving a cage within the same room or to another room, respectively.…”

Section: Introductionmentioning

confidence: 99%

Effects of Transfer from Breeding to Research Facility on the Welfare of Rats

Arts

Oosterhuis

Krämer

et al. 2014

Animals

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Simple SummaryThis study measured effects of transfer on body temperature, stress hormone levels, body weight, behavior and water and food intake in rats. Environmental temperature strongly affected body temperature of rats and needs to be controlled. Male rats need to habituate for at least one week, females for two weeks after transfer.AbstractTransfer from the breeding facility to a research facility is a stressful event for laboratory animals. Heat stress has been reported to constitute one of the major concerns during transport of animals. This study measured ambient and body temperature, corticosterone and glucose levels, body weight, behavior and water and food intake before, during and after transfer in Wistar rats. Decreased body weight, water and food intake were observed on the day of transfer in rats. Environmental temperature strongly affected body temperature of rats and needs to be controlled. Male rats need to habituate for at least one week, females for two weeks after transfer.

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“…[11][12][13] Although handling is accepted as stressful to mice, there is no total agreement as to whether mice can be habituated to handling with a meaningful reduction in the stress response. 11,14 Handling is made up of many variables including the specific technique of how the mouse is captured and other factors such as the handler and the environment. Moreover, differences in sensory adaptations between humans and mice render the scientist unaware of many environmental variables, such as auditory and olfactory stimuli, that can affect experimental conditions.…”

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Tail‐Cuff Technique and Its Influence on Central Blood Pressure in the Mouse

Wilde

Aubdool

Thakore

et al. 2017

JAHA

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BackgroundReliable measurement of blood pressure in conscious mice is essential in cardiovascular research. Telemetry, the “gold‐standard” technique, is invasive and expensive and therefore tail‐cuff, a noninvasive alternative, is widely used. However, tail‐cuff requires handling and restraint during measurement, which may cause stress affecting blood pressure and undermining reliability of the results.Methods and ResultsC57Bl/6J mice were implanted with radio‐telemetry probes to investigate the effects of the steps of the tail‐cuff technique on central blood pressure, heart rate, and temperature. This included comparison of handling techniques, operator's sex, habituation, and influence of hypertension induced by angiotensin II. Direct comparison of measurements obtained by telemetry and tail‐cuff were made in the same mouse. The results revealed significant increases in central blood pressure, heart rate, and core body temperature from baseline following handling interventions without significant difference among the different handling technique, habituation, or sex of the investigator. Restraint induced the largest and sustained increase in cardiovascular parameters and temperature. The tail‐cuff readings significantly underestimated those from simultaneous telemetry recordings; however, “nonsimultaneous” telemetry, obtained in undisturbed mice, were similar to tail‐cuff readings obtained in undisturbed mice on the same day.ConclusionsThis study reveals that the tail‐cuff technique underestimates the core blood pressure changes that occur simultaneously during the restraint and measurement phases. However, the measurements between the 2 techniques are similar when tail‐cuff readings are compared with telemetry readings in the nondisturbed mice. The differences between the simultaneous recordings by the 2 techniques should be recognized by researchers.

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Effect of Conditioning on the Increase of Heart Rate and Body Temperature Provoked by Handling in the Mouse

Cited by 26 publications

References 21 publications

Does the Stress Inherent to Laboratory Life and Experimentation on Animals Adversely Affect Research Data?

Does the Stress Inherent to Laboratory Life and Experimentation on Animals Adversely Affect Research Data?

Effects of Transfer from Breeding to Research Facility on the Welfare of Rats

Tail‐Cuff Technique and Its Influence on Central Blood Pressure in the Mouse

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