Effect of Confinement Stress on Circulating Levels of Growth Hormone and Two Prolactins in Freshwater-Adapted Tilapia (Oreochromis niloticus)

Aupérin, Benoît; Baroiller, Jean‐François; Ricordel, Marie-José; Fostier, Alexis; Prunet, Patrick

doi:10.1006/gcen.1997.6938

Cited by 119 publications

(78 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The Nile tilapia showed mean basal plasma cortisol levels ranging from 16.43 to 39.22 ng/ml (Table 1), which is in accordance with basal levels described in other studies for this and other fish species. Basal cortisol levels for the Nile tilapia ranging from 5 to 50 ng/ml and from 5 to 15 ng/ml were reported by Auperin et al (8) and by Barcellos et al (9), respectively. In a related cichlid, Oreochromis mossambicus, basal cortisol ranged from 20 to 60 ng/ml (11).…”

mentioning

confidence: 72%

“…The present study aims to understand the relation between welfare and environmental light color in the Nile tilapia, a fish where stress has been investigated (7)(8)(9) and which is considered to be the most extensively cultivated cichlid species in the world. Blue and green lights were tested because they are widespread in fish environments (1).…”

mentioning

confidence: 99%

“…Each fish was displaced by an opaque partition which confined it to a small area (less than 10% of the total aquarium volume) for 1 h, a method adapted from Auperin et al (8). This confinement was imposed in the morning and afternoon of two consecutive days.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Environmental blue light prevents stress in the fish Nile tilapia

Volpato

Barreto

2001

Braz J Med Biol Res

154

View full text Add to dashboard Cite

The present study aimed to test the effects of blue, green or white light on the stress response of the Nile tilapia, Oreochromis niloticus (L.). Each color was tested on two groups of isolated adult Nile tilapia (8 replicates each): one being subjected to confinement stress, and the other not (control). A different environmental color was imposed on each compartment by covering the light source with cellophane of the respective color (green or blue; no cellophane was used for white light). The intensity of green, white and blue lights was 250, 590 and 250 lux, respectively. Basal plasma cortisol levels were determined for each fish prior to the experimental procedures. The fish were confined by being displaced toward one side of the aquarium using an opaque partition for 1 h both in the morning and the afternoon of the two consecutive days of the test. At the end of this 48-h period, plasma cortisol levels were measured again. Basal cortisol levels (ng/ml) were similar for each group (ANOVA, F (2;42) = 0.77, P = 0.47). Thus, plasma cortisol levels were analyzed in terms of variation from their respective basal level. After confinement, plasma cortisol levels were not increased in fish submitted to a blue light environment. Thus, blue light prevents the confinement-induced cortisol response, an effect not necessarily related to light intensity.

show abstract

mentioning

confidence: 72%

mentioning

confidence: 99%

See 1 more Smart Citation

Environmental blue light prevents stress in the fish Nile tilapia

Volpato

Barreto

2001

Braz J Med Biol Res

154

View full text Add to dashboard Cite

show abstract

“…At the end of pairing, all fish were submitted to a second blood sampling for measurements after exposure to social stress (paired measures). The capture, anesthetic procedure, measurement, weighing and blood sampling (approximately 1 ml of blood) lasted a total of 2 min, based on the procedure reported by Auperin et al (19).…”

Section: Methodsmentioning

confidence: 99%

Effect of the establishment of dominance relationships on cortisol and other metabolic parameters in Nile tilapia (Oreochromis niloticus)

Corrêa

Fernandes

Iseki

et al. 2003

Braz J Med Biol Res

View full text Add to dashboard Cite

The objective of the present study was to investigate the influence of the establishment of dominance relationships and social stress on plasma cortisol and metabolite levels in Nile tilapia (Oreochromis niloticus). During the 30-day experiment, the fish weighing 236 ± 29 g were kept in individual aquaria, except for two pairings lasting 6 h each. Blood samples were taken from the animals before and after pairing. Display, approach, attack, rebuff, chase flight, and coloration were carried out on days 16 and 30. Activities and behaviors characteristic of the establishment of dominance relationships were described. It was possible to classify all experimental fish (N = 30) as dominant or subordinate. No differences were detected between dominant (N = 15) and subordinate (N = 15) fish during isolation or after pairing in cortisol (isolated: 5.76 ± 0.98 vs 5.42 ± 0.63; paired: 10.94 ± 1.62 vs 11.21 ± 2.45 µg/dl), glucose (isolated: 60.02 ± 4.9 vs 67. 85 ± 16.16; paired: 110.44 ± 15.72 vs 136.26 ± 22.46 mg/dl), triglyceride (isolated: 167.87 ± 5.06 vs 185.68 ± 7.24; paired: 210.85 ± 13.40 vs 221.82 ± 12.70 mg/dl) or total protein levels (isolated: 7.01 ± 0.42 vs 6.69 ± 0.59; paired: 9.21 ± 0.62 vs 9.51 ± 0.66 g/dl). However, when isolated (N = 30) and paired (N = 30) tilapia were compared, there were significant differences in cortisol and metabolite levels. The similar response presented by dominant and subordinate tilapia indicates that establishment of dominance relationships was a stressor for both groups.

show abstract

“…Moreover, the present study used mixed sex juveniles that had not reached sexual maturity and, therefore, sexual behaviors that contribute to stress in cultured fish are not yet expressed at this age in both sexes. Presently, HSD fish had mean cortisol value that is higher than the mean basal value described by Auperin et al (1997); and Volpato (2006, 2001) as normal for Nile tilapia. Thus, the cortisol data imply that HSD fish were stressed.…”

Section: Effect Of Stocking Density On Plasma Cortisol Level Blood Gmentioning

confidence: 58%

Effect of stocking density on the expression of glucose transporter protein 1 and other physiological factors in the Lake Victoria Nile tilapia, Oreochromis niloticus (L.)

et al. 2014

View full text Add to dashboard Cite

Fish farmers have a tendency of employing high stocking density (HSD) as a means of increasing productivity. However, HSD is a chronic stressor that is likely to lower profitability of fish farming if not implemented properly. HSD induces stress which in turn elevates sequentially the levels of plasma cortisol and glucose. The resultant glucose is distributed to various tissues by glucose transporter protein (GLUTs) to restore normalcy. GLUT 1, transmembrane protein found in erythrocytes, is responsible for import and export of glucose in red blood cells. However, knowledge on how chronic stress impact glucose and GLUT 1 protein in fish subjected to HSD is still unclear. In this study, effect of HSD on the expression of GLUT 1 in Nile tilapia was investigated in an attempt to elucidate the role of GLUT 1 in glucose metabolism during chronic stress. Fish were reared for 4 weeks at 1.5 and 4.5 kg/m 3 for low stocking density (LSD) and HSD, respectively. Four physiological parameters were determined from the blood samples obtained from fish at the end of experiment. At p \ 0.05, there were significant differences between fish reared at HSD and LSD in plasma cortisol level (72.1 ± 5.9 ng/ml and 37.5 ± 4.6 ng/ml); blood glucose level (136.00 ± 1.3 mg/dL and 70.2 ± 1.0 mg/dL); erythrocytes count (7.2 ± 0.5 9 106 mm -3 and 2.1 ± 0.4 9 106 mm -3 ); and plasma GLUT 1 level (1.40 ± 0.17 rbi and 0.81 ± 0.07 rbi), respectively. HSD induced elevation of plasma cortisol level, blood glucose level, erythrocytes count and GLUT 1 level. These elevated physiological factors and particularly GLUT 1 can be used as a cellular stress biomarker in fish farming and aquaculture.

show abstract

Effect of Confinement Stress on Circulating Levels of Growth Hormone and Two Prolactins in Freshwater-Adapted Tilapia (Oreochromis niloticus)

Cited by 119 publications

References 49 publications

Environmental blue light prevents stress in the fish Nile tilapia

Environmental blue light prevents stress in the fish Nile tilapia

Effect of the establishment of dominance relationships on cortisol and other metabolic parameters in Nile tilapia (Oreochromis niloticus)

Effect of stocking density on the expression of glucose transporter protein 1 and other physiological factors in the Lake Victoria Nile tilapia, Oreochromis niloticus (L.)

Contact Info

Product

Resources

About