Effect of conjugated linoleic acid on body composition in mice

Abstract

The effects of conjugated linoleic acid (CLA) on body composition were investigated. ICR mice were fed a control diet containing 5.5% corn oil or a CLA-supplemented diet (5.0% corn oil plus 0.5% CLA). Mice fed CLA-supplemented diet exhibited 57% and 60% lower body fat and 5% and 14% increased lean body mass relative to controls (P < 0.05). Total carnitine palmitoyltransferase activity was increased by dietary CLA supplementation in both fat pad and skeletal muscle; the differences were significant for fat pad of fed mice and skeletal muscle of fasted mice. In cultured 3T3-L1 adipocytes CLA treatment (1 x 10(-4)M) significantly reduced heparin-releasable lipoprotein lipase activity (-66%) and the intracellular concentrations of triacylglyceride (-8%) and glycerol (-15%), but significantly increased free glycerol in the culture medium (+22%) compared to control (P < 0.05). The effects of CLA on body composition appear to be due in part to reduced fat deposition and increased lipolysis in adipocytes, possibly coupled with enhanced fatty acid oxidation in both muscle cells and adipocytes.

Metabolic diversity in biohydrogenation of polyunsaturated fatty acids by lactic acid bacteria involving conjugated fatty acid production

“…Dietary CLA has been reported to reduce carcinogenesis (Ha et al 1987;Ha et al 1990;Pariza and Ha 1990;Ip et al 1991), atherosclerosis (Lee et al 1994), and body fat (Park et al 1997). Recently, similar effects were found for conjugated trienoic acids.…”

Nutritional therapy improves function and complements corticosteroid intervention in mdx mice

“…5 The retroperitoneal fat pad results strongly support the established ability of CLA to reduce adipose store accumulation in mice without altering total body weight. 34,35 Each of the interventions containing CLA reduced the retroperitoneal fat pad weights compared to PLTR. In addition, CrM, which increases fat free mass, 35,53 and ␣-LA, which influences glucose and lipid metabolism, 8,17 also reduced visceral adiposity.…”

“…34,35 Each of the interventions containing CLA reduced the retroperitoneal fat pad weights compared to PLTR. In addition, CrM, which increases fat free mass, 35,53 and ␣-LA, which influences glucose and lipid metabolism, 8,17 also reduced visceral adiposity. Although the effects of CLA in humans remain controversial, two studies have found that CLA reduced body fat mass in overweight humans, 1,13 suggesting that it may be an adjunctive therapy for attenuating corticosteroid-induced fat gain in boys with DMD.…”

“…It produces several potentially beneficial biological effects that are isomer and dose dependent. 53,54 For example, in rodents, CLA deceases body fat accretion by inhibiting adipocyte lipoprotein lipase activity, 34,35 enhances immune function, 54 suppresses carcinogenesis, 20 scavenges free radicals, 54 and possesses anti-inflammatory activity in bone. 24 〈lpha-lipoic acid (␣-LA) is a disulfide mitochondrial coenzyme and potent antioxidant that increases mitochondrial function and metabolic rates in aged rats.…”

Conjugated linoleic acid (CLA) and obesity

“…Trans-10,cis-12 CLA also inhibits the expression of glucose transporter-4 (GLUT-4) 37,38 and increases the activity of carnitine palmitoyltransferase (CPT), enhancing fatty acid oxidation 10 . Trans-10,cis-12 CLA reduces lipogenesis in human 37 and mice adipocytes, decreasing sterol regulatory element-binding protein-1 (SREBP-1) expression in vivo 39 .…”