Effect of Continuous Administration of Enalapril Maleate on the Oocyte Quality and In Vitro Production of Parthenote Embryos in Nulliparous and Multiparous Goats Undergoing Serial Laparoscopic Ovum Pick-Up

Abstract

Simple SummaryOne of the main obstacles to the in vitro production of embryos in goats is the low ovarian response to hormonal treatments and low oocyte quality. Thus, several strategies have been performed to improve the reproductive performance of goats, including the development of new hormonal protocols as well as the use of other drugs that act directly or indirectly on reproductive function. In this experiment, we tested the use of a hormonal protocol aimed at maximizing the ovarian response and, in parallel, an angiotensin-converting enzyme (ACE) inhibitor was administered daily as an adjuvant. In recent years, the renin-angiotensin system has been shown to play an important role in reproductive function, especially in follicular development. We found that administration of the ACE inhibitor affected the ovarian response in multiparous goats, with more visible follicles, and had no effect on oocyte quality or during embryonic development, thus being a possible alternative to improve goat reproductive response.AbstractThe aim of this work was to determine the effect of enalapril maleate administration, during oocyte recovery by serial laparoscopic ovum pick-up (LOPU), on the ovarian response and in vitro embryo production (IVP). Twenty cross-bred goats were allocated equally into two groups: Nulliparous and Multiparous. In each group, five animals were selected to receive daily doses of enalapril maleate during the hormonal protocol. Estrus was synchronized by a PGF2α analog, followed 48 h later by insertion of an intravaginal device with progesterone. Forty-eight hours after, a single dose of FSH/eCG was administered. The FSH/eCG doses were repeated three times, on every four day. Oocytes were recovered by LOPU 24 h after each FSH/eCG dose. Viable oocytes were matured in vitro, to be parthenogenetically activated and cultured for 72 h to the cleavage stage. The drug treatment increased the proportion of total follicles observed at LOPU (p < 0.01) in multiparous goats. In both parity groups, enalapril administration had no effect on the proportion or quality of oocytes recovered. Furthermore, the number of embryos cleaved was similar between the groups. Thus, enalapril maleate affected the ovarian response in multiparous animals only and had no effect on the oocyte quality or IVP.

Impact Of Hypotensive Action Of Angiotensin Converting Enzyme Inhibitor On Ovarian-Intraovarian Blood Flow And Follicles Development In Goats Hormonally Stimulated With Repeated Fsh-Ecg Treatment

“…The present study represents an effort to investigate how vasodilator action of ACE inhibitor affect the ovarian and intraovarian blood flow system and follicular dynamic following a primary evaluation focused on embryo production and oocyte quality [4].…”

“…The follicular development was stimulated by the i.m. administration, at the same time, of a single dose of 60 mg of FSH (Folltropin ® ) 4 plus a single dose of eCG (300 UI, Novormon 5000 ® ) 5 . The first FSH/eCG doses were administered 48 h after the dispositive insertion, being repeated every 4 days to complete three treatments ( Figure 1).…”