This paper examines whether transaction costs affect farmers' participation in contract farming focusing in Tanzania’s tea subsector. The study was descriptive by design and used primary data collected in a cross-sectional survey from 393 smallholder tea farmers from two regions (Mbeya and Njombe) in Tanzania. Binary Logistic Regression model was used to estimate the effect of transaction cost determinants on farmer’s participation in contract farming, focusing on lower and upper tea value chain nodes. Findings show that downward transaction costs significantly negatively impact contract farming participation (P=0.002), while upward transaction costs significantly positively influence participation (P=0.000). Specific downward transaction costs that significantly negatively influence contract farming participation at P=0.05 are time used to understand contract terms, and services delivery waiting time. Moreover, cost to know contract opportunities and terms, visiting frequency to the investor to qualify for contract farming, contract terms rigidity, and contract terms clarity, negatively influence participation but they are not statistically significant at P=0.05. We recommend that, to enhance farmers’ participation in contract farming, practitioners and policies should prioritize on reduction of specific downward transaction costs through training farmers and developing transaction cost-cutting policies. Future research can explore transaction costs in contract farming among processors and analysing the reasons for its variations across value chain nodes.