Effect of contrast media on vascular smooth muscle cells

Takatsuki, Hideyo; Furukawa, Tomoyasu; Liu, Yiyao; Hirano, Kunihiro; Yoshikoshi, Akio; Sakanishi, Akio

doi:10.1080/02841850410001079

Cited by 3 publications

(3 citation statements)

References 31 publications

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“…Thus, this concentration was used in the succeeding experiments. In previous studies, microbubbles exhibited side effects when injected into patients [21][22][23] . Our experiments demonstrated that the side effects of SonoVue microbubbles were not as evident as those of other reported ultrasound contrast media.…”

Section: Resultsmentioning

confidence: 99%

Vascular gene transfer and drug delivery in vitro using low-frequency ultrasound and microbubbles

Yang

Liu

et al. 2010

Acta Pharmacol Sin

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Aim: To determine the effects of ultrasound exposure in combination with a microbubble contrast agent (SonoVue) on the cellular uptake and delivery of drugs/genes into human umbilical vein endothelial cells (HUVECs) as well as their biological effects on migration. Methods: HUVECs in suspension were exposed to pulsed ultrasound with a 10% duty cycle in combination with various concentrations of a microbubble contrast agent (SonoVue) using a digital sonifier at a frequency of 20 kHz and an intensity of 3.77 W/cm 2 on the surface of a horn tip. Cell culture inserts were used to determine the cell migration ability. Results: Exposure to pulsed ultrasound resulted in enhanced green fluorescent protein (EGFP) gene transfection efficiencies ranging from 0.2% to 2%. The transfection efficiency of HUVECs was approximately 3-fold higher in the presence of SonoVue than in its absence at the effective exposure time of 6 s. For drug delivery to HUVECs using ultrasound, the delivery efficiencies of a lowmolecular-weight model drug (TO-PRO ® -1, M W 645.38) were significantly higher when compared to drug delivery without ultrasound, with a maximum efficiency of approximately 34%. However, the delivery efficiencies of a high-molecular-weight model drug (DextranRhodamine B, M W 70 000) were low, with a maximum delivery efficiency of nearly 0.5%, and gene transfection results were similarly poor. The migration ability of HUVECs exposed to ultrasound was also lower than that of the control (no exposure). Conclusion: The use of low-frequency and low-energy ultrasound in combination with microbubbles could be a potent physical method of increasing drug/gene delivery efficiency. This technique is a promising nonviral approach that can be used in cardiovascular disease therapy.

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Section: Resultsmentioning

confidence: 99%

Vascular gene transfer and drug delivery in vitro using low-frequency ultrasound and microbubbles

Yang

Liu

et al. 2010

Acta Pharmacol Sin

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“…CM normally induces dilation in healthy subjects [82,83], however, in CAD patients who typically exhibit endothelial dysfunction, coronary vasoconstriction or even vasospasm may occur [83,84]. In vitro studies have demonstrated that CM may exert negative effects on both EC [85,86] and VSMC [87,88]. CM is more likely to exert dose-dependent [89] vasoconstriction in segments close to a stenotic lesion [83,90], emphasizing a possible involvement of endothelial dysfunction, and constriction occurs more frequently following ionic CM than the currently used non-ionic CM [91].…”

Section: Does Contrast Media Using During Ptca/pci Cause Vascular Dysmentioning

confidence: 99%

Effects of Catheterization on Artery Function and Health: When Should Patients Start Exercising Following Their Coronary Intervention?

2019

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Coronary artery disease (CAD) is a leading cause of death worldwide, and percutaneous transluminal coronary angiography (PTCA) and/or percutaneous coronary intervention (PCI; angioplasty) are commonly used to diagnose and/or treat the obstructed coronaries. Exercise-based rehabilitation is recommended for all CAD patients; however, most guidelines do not specify when exercise training should commence following PTCA and/or PCI. Catheterization can result in arterial dysfunction and acute injury, and given that fact that exercise, particularly at higher intensities, is associated with elevated inflammatory and oxidative stress, endothelial dysfunction and a pro-thrombotic milieu, performing exercise post-PTCA/PCI may transiently elevate the risk of cardiac events. This review aims to summarize extant literature relating to the impacts of coronary interventions on arterial function, including the time-course of recovery and the potential deleterious and/or beneficial impacts of acute versus long-term exercise. The current literature suggests that arterial dysfunction induced by catheterization recovers 4-12 weeks following catheterization. This review proposes that a period of relative arterial vulnerability may exist and exercise during this period may contribute to elevated event susceptibility. We therefore suggest that CAD patients start an exercise-training program between 2 and 4 weeks post-PCI, recognizing that the literature suggest there is a "grey area" for functional recovery between 2-12 weeks post catheterization. The timing of exercise onset should take into consideration the individual characteristics of patients (age, severity of disease, comorbidities) and the intensity, frequency and duration of the exercise prescription.

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“…Iohexol, a non-ionic contrast medium having low osmotic pressure, shows a high glomerulus filtration rate and is a safe contrast medium with little direct side effects on the tissue and cells, compared with other iodized contrast media, thus typically making it available for use in both humans and animals in clinical fields (Barkin et al, 1991). However, it is reported that iohexol with known safety is toxic for vascular endothelium cells (Takatsuki et al, 2004;Zhang et al, 2000) and renal cells (Duan et al, 2006;Gong et al, 2010;Lee et al, 2006). While numerous reports on the effects of iohexol on several types of cells are made available recently, research reports on its effects on chondrocytes are not to be found in the medical area as well as in the veterinary science.…”

Section: Introductionmentioning

confidence: 95%

Cytotoxicity of Contrast Media Iohexol on IL-1beta stimulated Bovine Chondrocytes

Hong

Hwang

Kim

2018

J Hellenic Vet Med Soc

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ABSTRACT. The aim of this study was to determine the cytotoxic and metabolic effects of iohexol on cultured bovine chondrocytes in clinical dose. Chondrocytes were exposed to 50%, 25% and 12.5% iohexol and 50% mannitol for 2 hours. Cell proliferation, apoptosis and necrosis were analyzed. Real time PCR was performed for aggrecan, collagen type I and II gene expression. Cells in alginate beads stimulated by interleukin-1β (IL-1β) were analyzed for cytotoxicity. MTT assay showed that 50% iohexol inhibited the proliferation of cells at 2 hours culture period. Propidium iodide results showed significantly higher dead cells at 50% iohexol compared to control, however PCR results revealed that chondrogenic gene were not affected. Cells in alginate beads stimulated with IL-1β showed significantly higher percentage of dead cells at 50% iohexol exposure (p<0.05). These results suggested that iohexol has a cytotoxicity on chondrocytes and this cytotoxic effect possibly increased in inflammatory joint diseases.

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Effect of contrast media on vascular smooth muscle cells

Abstract: Ionic IOT is more cytotoxic to VSMCs than non-ionic IOH. However, the cytotoxicity was minimal and similar between both CMs at 1 mmol/l Ca2+ in accordance with the sodium-calcium balance.

Cited by 3 publications

References 31 publications

Vascular gene transfer and drug delivery in vitro using low-frequency ultrasound and microbubbles

Vascular gene transfer and drug delivery in vitro using low-frequency ultrasound and microbubbles

Effects of Catheterization on Artery Function and Health: When Should Patients Start Exercising Following Their Coronary Intervention?

Cytotoxicity of Contrast Media Iohexol on IL-1beta stimulated Bovine Chondrocytes

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