Effect of Controlled Local Acetylsalicylic Acid Release on in vitro Platelet Adhesion to Vascular Grafts

Hall, John D.; Rittgers, Stanley E.; Schmidt, Steven

doi:10.1177/088532829400800404

Cited by 8 publications

(3 citation statements)

References 41 publications

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“…The second burst release is observed only with the modified, DSC functionalized PCL samples and seems to be due to the cleavage of the hydrolyzable bond between PCL and ASA. These observations and further investigations hint at the possibility to achieve a partially controlled release of ASA by chemical coupling 63…”

Section: Discussionmentioning

confidence: 61%

Surface functionalization of poly(ε‐caprolactone) improves its biocompatibility as scaffold material for bioartificial vessel prostheses

et al. 2011

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Within this study, chemically modified polymer surfaces were to be developed, which should enhance the subsequent immobilization of various bioactive substances. To improve the hemocompatibility and endothelialization of poly(ε-caprolactone) (PCL) intended as scaffold material for bioartificial vessel prostheses, terminal amino groups via ammonia (NH₃) plasma, oxygen (O₂) plasma/aminopropyltriethoxysilane (APTES), and 4,4'-methylenebis(phenyl isocyanate) (MDI)/water were provided. Then, immobilization of the anti-inflammatory and antithrombogenic model drug acetylsalicylic acid (ASA) and vascular endothelial growth factor (VEGF) were performed by employing N,N-disuccinimidyl carbonate (DSC) as crosslinker. Contact angle and fluorescence measurements, X-ray photoelectron spectroscopy and infrared spectroscopy confirmed the surface modification. Here the highest functionalization was observed for the O₂ plasma/APTES modification. Furthermore, biocompatibility studies demonstrated that the surface reactions have no negative influence, neither on the viability of L929 mouse fibroblasts, nor on primary or secondary hemostasis. Release studies showed that the immobilization of ASA and VEGF on the modified PCL surface via DSC is greatly improved compared to the adsorption-only reference. The advantage of DSC is that it immobilizes both bioactive substances via non-hydrolyzable and/or hydrolyzable covalent bonding. The highest ASA loading and cumulative release was detected using NH₃ plasma-activated PCL samples. For VEGF, the O₂ plasma/APTES-modified PCL samples were most efficient with regard to loading and cumulative release. In conclusion, both modifications are promising methods to optimize PCL as scaffold material for bioartificial vessel prostheses.

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Section: Discussionmentioning

confidence: 61%

Surface functionalization of poly(ε‐caprolactone) improves its biocompatibility as scaffold material for bioartificial vessel prostheses

et al. 2011

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“…Additive manufacturing was used to prepare PCL-based devices loaded with ASA for cardiovascular applications. This drug has antiplatelet properties [ 29 , 30 , 31 , 32 , 33 ] and can be used to prevent blood clot formation on the surface of biomaterials [ 19 , 34 , 35 ]. Potential applications for the resulting material include the development of vascular grafts or catheters such as arteriovenous fistulas and central venous lines.…”

Section: Resultsmentioning

confidence: 99%

Use of 3D Printing for the Development of Biodegradable Antiplatelet Materials for Cardiovascular Applications

et al. 2021

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Small-diameter synthetic vascular grafts are required for surgical bypass grafting when there is a lack of suitable autologous vessels due to different reasons, such as previous operations. Thrombosis is the main cause of failure of small-diameter synthetic vascular grafts when used for this revascularization technique. Therefore, the development of biodegradable vascular grafts capable of providing a localized and sustained antithrombotic drug release mark a major step forward in the fight against cardiovascular diseases, which are the leading cause of death globally. The present paper describes the use of an extrusion-based 3D printing technology for the production of biodegradable antiplatelet tubular grafts for cardiovascular applications. For this purpose, acetylsalicylic acid (ASA) was chosen as a model molecule due to its antiplatelet activity. Poly(caprolactone) and ASA were combined for the fabrication and characterization of ASA-loaded tubular grafts. Moreover, rifampicin (RIF) was added to the formulation containing the higher ASA loading, as a model molecule that can be used to prevent vascular prosthesis infections. The produced tubular grafts were fully characterized through multiple techniques and the last step was to evaluate their drug release, antiplatelet and antimicrobial activity and cytocompatibility. The results suggested that these materials were capable of providing a sustained ASA release for periods of up to 2 weeks. Tubular grafts containing 10% (w/w) of ASA showed lower platelet adhesion onto the surface than the blank and grafts containing 5% (w/w) of ASA. Moreover, tubular grafts scaffolds containing 1% (w/w) of RIF were capable of inhibiting the growth of Staphylococcus aureus. Finally, the evaluation of the cytocompatibility of the scaffold samples revealed that the incorporation of ASA or RIF into the composition did not compromise cell viability and proliferation at short incubation periods (24 h).

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“…Furthermore, aspirin has low solubility in water and is easily decomposed by hydrolysis, making it a relevant model drug to use in loading and release studies, while keeping costs low. The release of aspirin from the micro/nanospheres can be controlled by both drug diffusion and polymer degradation, and it was dependent on the composition of the block polymer and the release medium 46–55…”

Section: Introductionmentioning

confidence: 99%

Acetylsalicylic acid loading and release studies of the PMMA‐g‐polymeric oils/oily acids micro and nanospheres

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Hazer

et al. 2010

J of Applied Polymer Sci

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Poly(methyl methacrylate) (PMMA) and PMMA copolymers derived from plant oils (Polylinseed oil-g-PMMA, Polysoybean oil-g-PMMA, Polylinoleic acidg-PMMA (PLina-g-PMMA) and Polyhydroxy alkanoatesy-g-Polylinoleic acid-g-PMMA (PHA-g-PLina-g-PMMA)) as hydrophobic polymers, a series of hydrophobic microsphere or nanosphere dispersions, were prepared by the emulsion/solvent evaporation method. The diameters of the nanospheres and microspheres were measured by dynamic light scattering with a zetasizer, optically and by scanning electron microscopy. The magnetic quality of the microspheres was determined by the electron spin resonance technique. Acetylsalicylic acid (aspirin, ASA) was used as a model drug and loaded into the microspheres during the preparation process. The effect of the stirring rate over the size and size distribution of the micro/ nanospheres was evaluated, and the effects of copolymer types derived from plant oil/oily acids and the copolymer/drug ratios were evaluated.

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Effect of Controlled Local Acetylsalicylic Acid Release on in vitro Platelet Adhesion to Vascular Grafts

Cited by 8 publications

References 41 publications

Surface functionalization of poly(ε‐caprolactone) improves its biocompatibility as scaffold material for bioartificial vessel prostheses

Surface functionalization of poly(ε‐caprolactone) improves its biocompatibility as scaffold material for bioartificial vessel prostheses

Use of 3D Printing for the Development of Biodegradable Antiplatelet Materials for Cardiovascular Applications

Acetylsalicylic acid loading and release studies of the PMMA‐g‐polymeric oils/oily acids micro and nanospheres

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