Effect of copaiba oil in hepatic damage induced by acetaminophen in rats

Abstract

PURPOSE:To investigate the effects of copaiba oil on the hepatic damage induced by paracetamol.METHODS Thirty six rats were distributed into six study groups (N=6): control group, that didn't receive the acetaminophen; Acetaminophen Group, that only received the acetaminophen; Prophylactic Copaiba Group 1, that received copaiba oil two hours before the acetaminophen; Prophylactic Copaiba Group 7, that received copaiba oil seven days, once by day, before the acetaminophen; Therapy Copaiba Group, that received the copaiba oil two hours afther the acetaminophen; and N-Acetyl-Cysteine Group, , that received the N-Acetyl-Cysteine two hours afther the acetaminophen. Euthanasia was performed after 24 hours. The serum levels of AST, ALT, alkaline phosphatase, GT, total bilirubin, direct bilirubin and indirect bilirubin and histological analisis were analized. RESULTS:The prophylactic copaiba group 7, therapy copaiba group and N-Acetyl-Cysteine Group showed amounts of AST and ALT similar to the control group; and the prophylactic copaiba group 1 showed similar levels to the acetaminophen group. There was no significant difference between the groups regarding the amount of alkaline phosphatase and GT (p>0.05). The therapy copaiba group showed the highest levels of bilirubin and was statistically different from the other groups (p<0.01) and this increased the costs of direct bilirubin. Regarding histopathology, the oil of copaiba administered prophylactic or therapeutic form for 7 days could decrease the amount of necrosis and inflammatory infiltrate. CONCLUSION:Copaiba oil administered prophylactically for seven days, and therapeutic could reduce liver damage caused by paracetamol similarly N-Acetyl-Cysteine, however, when treated with copaiba therapeutically showed increases in bilirubin, costs increasing fraction indirect.

Effects of the topical administration of copaiba oil ointment (Copaifera langsdorffii) in skin flaps viability of rats

“…The mechanism of action of the active ingredients in copaíba oil is still not yet totally clear 29 . The majority of its antibacterial and anti-fungal properties is related to the products of secondary metabolism, found in essential oils such as terpenoids and phenolic counmponds, which also in their pureform exhibit antimicrobe properties.…”

Attenuation of copaiba oil in hepatic damage in rats

“…In our previous study 16 it was proposed that copaiba oil decreased levels of liver injury induced by acetaminophen, reinforcing the herbal anti-inflammatory effect. Nevertheless, to ensure the safety and the effectiveness of this oil its necessary to know detailed mechanism of this herbal, before applying it in large animal models.…”

“…In our previous study, copaiba oil showed a protective effect against liver injury; however it was not possible to identify the factors that led to decreased damage and a more detailed understand about the histopathological changes in damaged liver 16 . Thus, it was necessary to expand the study to elucidate some questions about the mechanism of this hepatoprotective effect.…”

Copaiba oil effect on induced fecal peritonitis in rats

“…Is believed then that the anti-inflammatory protective effect occurred only copaiba prophylactically, without providing effect on survival of animals when administered alone after sepsis or increasing some protection together with prophylaxis 12,14,17,18 .…”