Effect of Coronary Percutaneous Revascularization on Interferon-γ and Interleukin-10 Producing CD4⁺ T Cells during Acute Myocardial Infarction

Abstract

T lymphocytes play an important role in the induction and progression of acute coronary syndromes (ACS). To gain insight into how different T cell subsets can influence ACS, we analyzed the frequencies of circulating CD4+T cells producing either pro-inflammatory interferon(IFN)-y or anti-inflammatory interleukin (IL)-tO in subjects presenting with ST-elevation myocardial infarction (STEMI). The effect of coronary bare metal (BS) and paclitaxel-eluting stent (PES) on the balance between CD4+IFN-yand CD4+IL-tO+ lymphocytes was also investigated. Peripheral blood mononuclear cells (PBMC) were isolated from 38 consecutive patients with STEMI before and 48 hrs or 6 days after implantation of either BS or PES. Twenty patients with no history of coronary artery disease were included as basal controls.PBMC were stimulated in vitro with anti-CD3/anti-CD28 monoclonal antibodies, and CD4+IFN-y-or CD4+IL-tO+ T cells were detected by flow cytometry intracellular staining. The frequency of peripheral CD4+IL-tO+ T cells was significantly higher in STEMI patients as compared with controls. Conversely, the frequency of CD4+IFN-y-T lymphocytes did not differ between STEMI and subjects without history of coronary artery disease. Six days after the revascularization procedure, the percentage of CD4+IL-t 0+ T cells was significantly decreased in BS but not in the PES group, whereas the relative percentage of CD4+IFN-y-T lymphocytes were diminished in both groups as compared with baseline levels. Our data indicate that STEMI is associated with a peripheral expansion of CD4+IL-tO+T lymphocytes, and that primary coronary revascularization with implantation of either BS or PES is followed by a reduction in circulating CD4+IFN-y-T lymphocytes. PES implantation, however, appears to inhibit the relative decrease of the IL-t0 producing lymphocyte as observed in BS implanted patients, shifting the balance between pro-inflammatory and anti-inflammatory T cell populations in favor of the latter.Increasing evidence suggests that immune mediated mechanisms play a crucial role in the pathogenesis of acute coronary syndromes (ACS) (1-2). It is now accepted that the rupture of coronary atherosclerotic plaque depends on an inflammatory process rather than on plaque morphology (3). The finding that the percentage of activated T cells in the culprit lesions is significantly higher in ACS

A putative association of interleukin‐10 promoter polymorphisms with cardiovascular disease

“…The noted rise in IL-10 level could be due to the increased activation of proinflammatory interleukins that promotes the secretion of antiinflammatory IL-10 (23). The present study is in line with previous reports which showed an increase in IL-10 level in STEMI patients (24). In one study, Karpi nski et al (2008) reported the significant rise in IL-10 level in the acute phase of myocardial infarction (25).…”

Expression of the High-Affinity Laminin Receptor (67 kDa) in Normal Human Skin and Appendages

“…In the cardiovascular system this receptor is present on both adherent cardiovascular system cells and on circulating inflammatory cells (39)(40)(41)(42)(43)(44)(45)(46)(47)(48)(49)(50)(51)(52)(53) . It is involved in balancing degradation processes initiated by inflammatory cells and in the response of adherent cells to alterations in surrounding extra cellular matrix.…”

Substance P Upregulates LTB4 in Rat Adherent Macrophages from Granuloma Induced by KMnO4

“…Proinflammatory compounds are targeted for therapeutic intervention because of their role in promoting or sustaining harmful inflammation (Hansson 2005;Inoue et al 2008;Sardella et al 2007;Lavagno et al 2007;Bruzzese 2008). Substance P (SP) is an undecapeptide belonging to a group of proteins named tachykinins and is the key transmitter of pain to the brain (García-Horsman et al 2007;Katsanos et al 2008a;Katsanos et al 2008b).…”