Effect of cortisol treatment and/or sublethal copper exposure on copper uptake and heat shock protein levels in common carp, <i>Cyprinus carpio</i>

Boeck, Gudrun De; Wachter, B. De; Vlaeminck, Andrea; Blust, Ronny

doi:10.1002/etc.5620220521

Cited by 47 publications

(17 citation statements)

References 32 publications

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“…24 This gene can also be expressed in a tissue-specific manner. 25 This study found similar conclusions to those of De Boeck et al, 26 who reported that exposure of Cyprinus carpio to a Cu concentration of 1.9 mM in water induced hsp70 expression in the gills, erythrocytes and liver. Using the mRNA differential display technique, Carginale et al 27 also described changes in hsp70 expression in the liver of Chionodraco hamatus submitted to sublethal doses of Cd.…”

Section: Resultssupporting

confidence: 90%

Effects of Copper and Cadmium Exposure on mRNA Expression of Catalase, Glutamine Synthetase, Cytochrome P450 and Heat Shock Protein 70 in Tambaqui Fish (Colossoma Macropomum)

Casanova¹,

Honda²,

Ferreira-Nozawa³

et al. 2013

GGG

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Abstract:In this work the authors evaluated the expression of cat, glu, cypP450, hsp70 and 18S RNA in Colossoma macropomum following exposure to copper and cadmium by semi-quantitative RT-PCR and quantitative qRT-PCR. In RT-PCR and qRT-PCR hsp70 expression was highest after exposure to copper for 3 hours (h). glu expression increased after exposure to cadmium, regardless of time. cat expression increased after the first hour of exposure to both metals, and decreased after 3 hs. cyp450 expression was higher when compared to controls in exposed fish regardless of metal and exposure time. cyp450 expression after exposure to copper for 3 h was twice that recorded after 1 h. In the presence of cadmium, cyp450 expression was higher in the animals exposed for 1 h and lower at 3 h. Therefore, toxicology tests and biomonitoring based on molecular markers should focus on a set of molecular and/or environmental parameters, rather than isolated markers.

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Section: Resultssupporting

confidence: 90%

Effects of Copper and Cadmium Exposure on mRNA Expression of Catalase, Glutamine Synthetase, Cytochrome P450 and Heat Shock Protein 70 in Tambaqui Fish (Colossoma Macropomum)

Casanova¹,

Honda²,

Ferreira-Nozawa³

et al. 2013

GGG

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“…There were no effects on plasma parameters, including plasma cortisol, at 14 g/l Cu (Dethloff et al, 1999b). Given the controversy regarding the protective effect of cortisol during exposure to metals (Bury et al, 1998;De Boeck et al, 2003;Mazon et al, 2004), it is important to characterize the physiological stress response to chronic Cu exposure in fish and determine whether pre-exposure to Cu protects cells against Cu toxicity. Cortisol secretion by adrenocortical cells of rainbow trout and yellow perch is impaired by in vitro exposure to Cd or Hg (Leblond and Hontela, 1999;Lacroix and Hontela, 2004) and by chronic field exposures to metals including Cu in lakes situated in a mining region (Lévesque et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Effects of Cu on plasma cortisol and cortisol secretion by adrenocortical cells of rainbow trout (Oncorhynchus mykiss)

2006

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Fish are exposed to multiple stressors, often acting concurrently, in their environment. To evaluate the potential of Cu to act as a chemical stressor, rainbow trout (Oncorhynchus mykiss) were exposed to Cu (30 or 80 g/l) for 30 days in the laboratory and they were subjected to a physical stressor (1 min air exposure) before sampling. Physiological stress indicators in the whole fish as well as cortisol secretion by adrenocortical cells in vitro were measured. Fish exposed to Cu had a lower condition factor, hepatosomatic index, plasma glucose, hepatic glycogen and gill Na + /K + -ATPase activity compared to controls. Exposure to Cu did not have an effect on basal plasma cortisol (fish sampled without air exposure stress) however, the air exposure-induced increase in plasma cortisol was lower in fish exposed to Cu. Cortisol secretion stimulated by ACTH in vitro was greater in adrenocortical cells isolated from fish exposed to Cu in vivo but in vitro exposure to Cu consistently impaired cortisol secretion. Our results indicate that Cu at high concentrations disrupts cortisol secretion through a direct toxic effect on adrenocortical cells while low concentrations resulting from a 30-day exposure to environmentally relevant Cu concentrations enhances cortisol secretion in response to ACTH in vitro.

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“…The glucocorticoid receptor is chaperoned by several HSPs, mainly the HSP90 and HSP70 families (17), so it is perhaps not surprising that the receptor's main ligand, the stress hormone cortisol, also affects HSP gene and protein expression. Most evidence of cortisol's impact on HSPs comes from studies on fish (2,6,11,48), where much of the research points to a suppressive effect of cortisol on HSP induction at the mRNA and protein level.…”

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confidence: 99%

Hormonal modulation of the heat shock response: insights from fish with divergent cortisol stress responses

LeBlanc

Höglund

Gilmour

et al. 2012

American Journal of Physiology-Regulatory, Integrative and Comp

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LeBlanc S, Höglund E, Gilmour KM, Currie S. Hormonal modulation of the heat shock response: insights from fish with divergent cortisol stress responses. Am J Physiol Regul Integr Comp Physiol 302: R184-R192, 2012. First published October 26, 2011 doi:10.1152/ajpregu.00196.2011.-Acute temperature stress in animals results in increases in heat shock proteins (HSPs) and stress hormones. There is evidence that stress hormones influence the magnitude of the heat shock response; however, their role is equivocal. To determine whether and how stress hormones may affect the heat shock response, we capitalized on two lines of rainbow trout specifically bred for their high (HR) and low (LR) cortisol response to stress. We predicted that LR fish, with a low cortisol but high catecholamine response to stress, would induce higher levels of HSPs after acute heat stress than HR trout. We found that HR fish have significantly higher increases in both catecholamines and cortisol compared with LR fish, and LR fish had no appreciable stress hormone response to heat shock. This unexpected finding prevented further interpretation of the hormonal modulation of the heat shock response but provided insight into stress-coping styles and environmental stress. HR fish also had a significantly greater and faster heat shock response and less oxidative protein damage than LR fish. Despite these clear differences in the physiological and cellular responses to heat shock, there were no differences in the thermal tolerance of HR and LR fish. Our results support the hypothesis that responsiveness to environmental change underpins the physiological differences in stress-coping styles. Here, we demonstrate that the heat shock response is a distinguishing feature of the HR and LR lines and suggest that it may have been coselected with the hormonal responses to stress. heat shock protein; catecholamines; heat stress; thermal tolerance; high-responding; low-responding HEAT SHOCK PROTEINS (HSPs) are molecular chaperones responsible for the repair of damaged proteins and assist in folding of new proteins. They increase following cellular stress resulting from exposure to high temperature, ischemia-reperfusion, or oxidative damage, and they have been found in every organism in which they have been sought (13, 21). The putative trigger for HSP induction is an increase in damaged proteins within the cell (1), which frees the heat shock transcription factor (e.g., HSF1) to initiate rapid increases in gene transcription followed by translation into HSPs (31). Although an increase in damaged proteins is likely the primary trigger for HSP induction following stress; stress hormones, including catecholamines and cortisol, influence transcription and translation of HSP genes and proteins. Catecholamines appear to augment stress-induced HSP gene transcripts and proteins in mammals (32, 39, 54), fish (9), and invertebrates (23). The glucocorticoid receptor is chaperoned by several HSPs, mainly the HSP90 and HSP70 families (17), so it is perhaps not surprising tha...

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Effect of cortisol treatment and/or sublethal copper exposure on copper uptake and heat shock protein levels in common carp, Cyprinus carpio

Cited by 47 publications

References 32 publications

Effects of Copper and Cadmium Exposure on mRNA Expression of Catalase, Glutamine Synthetase, Cytochrome P450 and Heat Shock Protein 70 in Tambaqui Fish (Colossoma Macropomum)

Effects of Copper and Cadmium Exposure on mRNA Expression of Catalase, Glutamine Synthetase, Cytochrome P450 and Heat Shock Protein 70 in Tambaqui Fish (Colossoma Macropomum)

Effects of Cu on plasma cortisol and cortisol secretion by adrenocortical cells of rainbow trout (Oncorhynchus mykiss)

Hormonal modulation of the heat shock response: insights from fish with divergent cortisol stress responses

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