Effect of coverage density of the retention mechanism in reversed-phase high-performance liquid chromatography

Buszewski, Bogusław; Suprynowicz, Z.; Staszczuk, P.; Albert, Klaus; Pfleiderer, Bettina; Bayer, Ernst

doi:10.1016/s0021-9673(00)96981-4

Cited by 51 publications

(37 citation statements)

References 25 publications

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“…1) provide important information about the structure of chemically bonded silica phases ( sponds to the monomeric structure with one-point ligand-silanol group bond [35]. The synthesis of low coverage density stationary phase causes a decrease in Q 2 and Q 3 signal intensity (ca.…”

Section: Analysis Of Selenopeptidesmentioning

confidence: 99%

Effect of coverage density and structure of chemically bonded silica stationary phases on the separation of compounds with various properties

Buszewski¹,

Krupczyńska

Rychlicki

et al. 2006

J of Separation Science

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The chemical character, geometry, and architecture of chemically formed surface layers determine interactions between stationary phase, analyte, and mobile phase, and therefore the retention mechanisms (partitioning, adsorption, ion exchange, steric exclusion) of separated analytes. These interactions also depend on the structure and chemical character of the solutes and the composition of the mobile phase. High-molecular-weight fullerenes (C60 and C70) and water-soluble selenium-containing peptides (833 and 2607 Da) were used for the evaluation of laboratory-prepared octadecyl stationary phases with high and low coverage density before and after end-capping. The aim of this work was to study differences in surface coverage density and homogeneity and conformational changes of chemically bonded moieties and the influence of these parameters on the separation of mixtures of selenopeptides and fullerenes with significantly different molecular masses. A topographical model of the chemically modified stationary surface is presented.

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Section: Analysis Of Selenopeptidesmentioning

confidence: 99%

Effect of coverage density and structure of chemically bonded silica stationary phases on the separation of compounds with various properties

Buszewski¹,

Krupczyńska

Rychlicki

et al. 2006

J of Separation Science

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“…29 Si CP/MAS NMR has been used for these studies [8,51,52]. According to Bayer and co-workers [23,53], and Maciel and Sindorf [54], modification of the support surface with monosilane leads to the monomeric structure of CBP (chemical shift r= -2.5 ppm) ( Table 2). Using di-or trifunctional silanes more complicated systems are created, as shown in Figure 1.B.…”

Section: Structure and Type Of Modifiersmentioning

confidence: 99%

“…Consequently, a new adsorption site (3Si1OH group) localized on the bonded alkylsilyl ligands may negatively influence the RP HPLC separation process [23,53,54]. Only small differences have been observed between alkoxy-and chlorosilanes [53]. During the modification reaction, by-products (dimethylamine, methanol, and HCl) are released.…”

Section: Structure and Type Of Modifiersmentioning

confidence: 99%

Survey and Trends in the Preparation of Chemically Bonded Silica Phases for Liquid Chromatographic Analysis

Buszewski¹,

Jezierska

Wełniak

et al. 1998

J. High Resol. Chromatogr.

146

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“…Silica-based alkyl-bonded phases are the most popular stationary phases used in this method [1,2]. The length of bonded ligands, their coverage density and homogeneity indicates the hydrophobic properties of the bonded phase [3][4][5][6][7]. The most often used RP stationary phase is the octadecylsilane or C18 column, which provides a moderately long alkyl chain of 18 carbon atoms.…”

Section: Introductionmentioning

confidence: 99%

Modeling solvation on the chemically modified silica surfaces

Buszewski

Bocian

Nowaczyk

2010

J of Separation Science

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A detailed molecular-level description of the retention mechanism in RP-HPLC is a point of great interest to analytical chemists. For this purpose, the solvent adsorption on the octadecyl stationary-bonded phase was investigated. Preferential adsorption of solvents from the acetonitrile-water mobile phase was modeled on the silica surface with one, two, three and four organic ligands, which represents a series of non-end-capped-bonded phases with different coverage density of bonded ligands. As a result of the computer simulation, the increase of adsorbed acetonitrile around bonded ligands is observed. The number of water molecules near the modeled surface is observed as well. The results are in agreement with experimental measurement of acetonitrile excess adsorption isotherms on the series of in-house made stationary-bonded phase.

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Effect of coverage density of the retention mechanism in reversed-phase high-performance liquid chromatography

Cited by 51 publications

References 25 publications

Effect of coverage density and structure of chemically bonded silica stationary phases on the separation of compounds with various properties

Effect of coverage density and structure of chemically bonded silica stationary phases on the separation of compounds with various properties

Survey and Trends in the Preparation of Chemically Bonded Silica Phases for Liquid Chromatographic Analysis

Modeling solvation on the chemically modified silica surfaces

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