Effect of COVID-19 protective measures on the epidemiology characteristics of rotavirus, adenovirus, and coinfections among pediatric patients with acute gastroenteritis in Hangzhou, China

SADS-CoV, a coronavirus, is known to induce swine acute diarrhea syndrome. To explore the differences and commonalities in the pathogenesis mechanisms between highly pathogenic and low-pathogenic strains of SADS-CoV, we conducted an integrated analysis comprising proteomics, metabolomics, and short-chain fatty acid (SCFA) analysis, along with 16S rRNA sequencing of intestinal mucosa and fecal samples from piglets infected with SADS-CoV P7 (highly pathogenic)or SADS-CoV P83༈low-pathogenic༉. Additionally, we examined molecular events linked to potential pathogenicity and host immune responses subsequent to correlational analysis of diverse omics data. In the SADS-CoV P7-infected group, the abundance of unidentified members of the family Enterobacteriaceae was markedly greater than in either the control group or the SADS-CoV P83-infected group in the ileum mucosa and feces. The concentration of SCFAs was significantly lower in SADS-CoV P7-infected pigs than in SADS-CoV P83-infected pigs, and SCFA levels were negatively correlated with the abundance of Enterobacteriaceae and the abundance of the species Escherichia coli in the ileum mucosa. Compared to those in the SADS-CoV P83 group, the differentially expressed proteins in the SADS-CoV P7 group were predominantly linked to extracellular matrix (ECM)-receptor interactions and focal adhesion pathways. Following SADS-CoV P7 infection, there was an increase in both the adhesion force and the number of Escherichia coli O157 adherent to IPEC-J2 cells. Moreover, SADS-CoV P7 can modulate the adhesion of E. coli O157 to IPEC-J2 cells by regulating the expression of the ECM-related protein integrin alpha5 (ITGA5), suggesting that ITGA5 plays a pivotal role in the invasion of E. coli O157 into intestinal epithelial cells during SADS-CoV infection. A correlation exists among the multiomics profiles of the small intestinal mucosa and feces of piglets following infection with various generations of SADS-CoV. Understanding this correlation can help us better prevent the virus from harming piglets.

show abstract

Modern epidemiological features of viral acute intestinal infections in children and adolescents of Sakhalin region

Sapega,

Butakova,

Trotsenko

2024

jour

View full text Add to dashboard Cite

Objective: to identify modern epidemiological features of viral acute intestinal infections in children and adolescents in the Sakhalin Region.Materials and methods. Data on acute intestinal infection incidence obtained from statistical reports of the Rospotrebnadzor during 2021-2023 was compared with long-term average incidence rates calculated over the period before the COVID-19 pandemic (2010-2019) to perform epidemiological analysis. Incidence was calculated for different age groups; according to months of the year, monthly long-term average incidence rates were established. Index of seasonality, coefficient of seasonality, and index of seasonal growth were determined.Results and discussion. Manifestations of acute intestinal infections epidemic process in the Sakhalin Region indicate persisting conditions for an increase in the registration of both sporadic cases of diseases and outbreaks of infectious diarrhea. Viruses were prevailing in the structure of cases of known etiology (mainly caused by rotaviruses and noroviruses). Their intra-annual dynamics showed changes in rotavirus and norovirus infection incidence rates: a shift in periods of increased incidence, a decrease in the coefficient of seasonality, and for rotavirus infection, a decrease in the index of seasonal growth. Age-adjusted rotavirus infection incidence showed predominance of the infection among preschool-aged children; norovirus infection was frequently detected among schoolchildren and adolescents. An increase in the focality index may indicate ongoing problems in the public catering sector, non-compliance with sanitary rules and hygiene standards, and untimely detection and isolation of patients with acute intestinal infections.Conclusion. Obtained results confirm the epidemiological significance of viral gastroenteritis and the necessity of anti-epidemic measures optimization.

show abstract

Effect of COVID-19 protective measures on the epidemiology characteristics of rotavirus, adenovirus, and coinfections among pediatric patients with acute gastroenteritis in Hangzhou, China

Cited by 5 publications

References 39 publications

Comparative analysis of the epidemiological characteristics of adenovirus, rotavirus A, and coinfection in children during 2014–2023 in Guangzhou, China

Comparative analysis of the epidemiological characteristics of adenovirus, rotavirus A, and coinfection in children during 2014–2023 in Guangzhou, China

Multiomics Reveals Alterations in the Gut Microbiome, Host Proteins, and Host Metabolites Correlating with SADS-CoV Pathogenicity and the Immune Response in Piglets

Modern epidemiological features of viral acute intestinal infections in children and adolescents of Sakhalin region

Contact Info

Product

Resources

About