Effect of CRABP2 on the proliferation and odontoblastic differentiation of hDPSCs

Yan, Yanhong; Qi, Shengcai; Shuili, Gong; Shang, Guangwei; Zhao, Yanling

doi:10.1590/1807-3107bor-2017.vol31.0112

Cited by 5 publications

(5 citation statements)

References 25 publications

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“…Odontoblast differentiation is the prerequisite to dental tissue engineering and dental restoration. A number of studies have promoted the differentiation of DPSCs into odontoblasts using a variety of factors, including bone morphogenetic protein 2 [ 30 ], neuropilin-1-FYN [ 30 ], cellular retinoic acid-binding protein 2 [ 31 ] and intraflagellar transport 140 [ 32 ]. Dental pulp tissue is located in the closed and hard dentin wall, which determines its poor intramedullary circulation.…”

Section: Discussionmentioning

confidence: 99%

Different concentrations of C5a affect human dental pulp mesenchymal stem cells differentiation

Liu

Wei

et al. 2021

BMC Oral Health

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Background During the process of deep decay, when decay approaches the pulp, an immune response is triggered inside the pulp, which activates the complement cascade. The effect of complement component 5a (C5a) on the differentiation of dental pulp mesenchymal stem cells (DPSCs) is related to dentin reparation. The aim of the present study was to stimulate DPSCs with different concentrations of C5a and evaluate the differentiation of odontoblasts using dentin sialoprotein (DSP). Methods DPSCs were divided into the following six groups: (i) Control; (ii) DPSCs treated with 50 ng/ml C5a; (iii) DPSCs treated with 100 ng/ml C5a; (iv) DPSCs treated with 200 ng/ml C5a; (v) DPSCs treated with 300 ng/ml C5a; and (vi) DPSCs treated with 400 ng/ml C5a. Flow cytometry and multilineage differentiation potential were used to identify DPSCs. Mineralization induction, Real-time PCR and Western blot were conducted to evaluate the differentiation of odontoblast in the 6 groups. Result DPSCs can express mesenchymal stem cell markers, including CD105, CD90, CD73 and, a less common marker, mesenchymal stromal cell antigen-1. In addition, DPSCs can differentiate into adipocytes, neurocytes, chondrocytes and odontoblasts. All six groups formed mineralized nodules after 28 days of culture. Reverse transcription-quantitative PCR and western blotting indicated that the high concentration C5a groups expressed higher DSP levels and promoted DPSC differentiation, whereas the low concentration C5a groups displayed an inhibitory effect. Conclusion In this study, the increasing concentration of C5a, which accompanies the immune process in the dental pulp, has demonstrated an enhancing effect on odontoblast differentiation at higher C5a concentrations in vitro.

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Section: Discussionmentioning

confidence: 99%

Different concentrations of C5a affect human dental pulp mesenchymal stem cells differentiation

Liu

Wei

et al. 2021

BMC Oral Health

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show abstract

“…Odontoblast differentiation is the prerequisite to dental tissue engineering and dental restoration. A number of studies have promoted the differentiation of DPSCs into odontoblasts using a variety of factors, including bone morphogenetic protein 2 (30), neuropilin-1-FYN (30), cellular retinoic acid-binding protein 2 (31) and intra agellar transport 140 (32). Dental pulp tissue is located in the closed and hard dentin wall, which determines its poor intramedullary circulation.…”

Section: Discussionmentioning

confidence: 99%

Different Concentrations of C5a Affect Human Dental Pulp Mesenchymal Stem Cells Differentiation

Liu

Wei

et al. 2021

Preprint

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Background During the process of deep decay, when decay approaches the pulp, an immune response is triggered inside the pulp, which activates the complement cascade. The effect of complement component 5a (C5a) on the differentiation of dental pulp mesenchymal stem cells (DPSCs) is related to dentin reparation. The aim of the present study was to stimulate DPSCs with different concentrations of C5a and evaluate the differentiation of odontoblasts using dentin sialoprotein (DSP). Methods DPSCs were divided into the following six groups: i) Control; ii) DPSCs treated with 50 ng/ml C5a; iii) DPSCs treated with 100 ng/ml C5a; iv) DPSCs treated with 200 ng/ml C5a; v) DPSCs treated with 300 ng/ml C5a; and vi) DPSCs treated with 400 ng/ml C5a. Flow cytometry and multilineage differentiation potential were used to identify DPSCs. Mineralization induction, Real-time PCR and Western blot were conducted to evaluate the differentiation of odontoblast in the 6 groups.Result DPSCs can express mesenchymal stem cell markers, including CD105, CD90, CD73 and, a less common marker, mesenchymal stromal cell antigen-1. In addition, DPSCs can differentiate into adipocytes, neurocytes and osteoblasts. All six groups formed mineralized nodules after 28 days of culture. Reverse transcription-quantitative PCR and western blotting indicated that the high concentration C5a groups expressed higher DSP levels and promoted DPSC differentiation, whereas the low concentration C5a groups displayed an inhibitory effect.Conclusion In this study, the increasing concentration of C5a, which accompanies the immune process in the dental pulp, has demonstrated an enhancing effect on odontoblast differentiation at higher C5a concentrations in vitro.

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“…In murine tooth development, RAR β expression is initiated during bell stage and is seen in odontoblasts [ 37 ]. The expression of CRABP2 is seen in dental mesenchymal cells and decreases during dentin development in mouse [ 38 ]. CRABP2 knockdown enhances odontoblastic differentiation of human DPSCs.…”

Section: Discussionmentioning

confidence: 99%

“…Given the negative role of RA in mineralization, we propose that blocking RA signal may be an effective method for dentin regeneration from DPSCs. Knocking down the CRABP2 in DPSCs through transfection with lentivirus promoted the odontogenic differentiation of DPSCs in vitro [ 38 ]. However, procedural difficulties and safety issues are the roadblocks in the clinical application of lentivirus transfection.…”

Section: Discussionmentioning

confidence: 99%

Retinoic Acid Signal Negatively Regulates Osteo/Odontogenic Differentiation of Dental Pulp Stem Cells

Wang

et al. 2020

Stem Cells International

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Retinoic acid (RA) signal is involved in tooth development and osteogenic differentiation of mesenchymal stem cells (MSCs). Dental pulp stem cells (DPSCs) are one of the useful MSCs in tissue regeneration. However, the function of RA in osteo/odontogenic differentiation of DPSCs remains unclear. Here, we investigated the expression pattern of RA in miniature pig tooth germ and intervened in the RA signal during osteo/odontogenic differentiation of human DPSCs. Deciduous canine (DC) germs of miniature pigs were observed morphologically, and the expression patterns of RA were studied by in situ hybridization (ISH). Human DPSCs were isolated and cultured in osteogenic induction medium with or without RA or BMS 493, an inverse agonist of the pan-retinoic acid receptors (pan-RARs). Alkaline phosphatase (ALP) activity assays, alizarin red staining, quantitative calcium analysis, CCK8 assay, osteogenesis-related gene expression, and in vivo transplantation were conducted to determine the osteo/odontogenic differentiation potential and proliferation potential of DPSCs. We found that the expression of RARβ and CRABP2 decreased during crown calcification of DCs of miniature pigs. Activation of RA signal in vitro inhibited ALP activities and mineralization of human DPSCs and decreased the mRNA expression of ALP, osteocalcin, osteopontin, and a transcription factor, osterix. With BMS 493 treatment, the results were opposite. Interference in RA signal decreased the proliferation of DPSCs. In vivo transplantation experiments suggested that osteo/odontogenic differentiation potential of DPSCs was enhanced by inversing RA signal. Our results demonstrated that downregulation of RA signal promoted osteo/odontogenic differentiation of DPSCs and indicated a potential target pathway to improve tissue regeneration.

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Effect of CRABP2 on the proliferation and odontoblastic differentiation of hDPSCs

Cited by 5 publications

References 25 publications

Different concentrations of C5a affect human dental pulp mesenchymal stem cells differentiation

Different concentrations of C5a affect human dental pulp mesenchymal stem cells differentiation

Different Concentrations of C5a Affect Human Dental Pulp Mesenchymal Stem Cells Differentiation

Retinoic Acid Signal Negatively Regulates Osteo/Odontogenic Differentiation of Dental Pulp Stem Cells

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