“…The amount of evolved CO 2 from powdered liquorice root refuse of Rishmak, Zarghan, and Shirin-Daroo factories, wheat straw and alfalfa were 51.91, 52.27, 51.70, 55.17, and 63.39 mg (20 g) 21 of soil, respectively ( Table 6). Stotzky and Mortensen (12), and Maftoun and Banihashemi (14), confirmed that the lower production of CO 2 in soils amended with wheat straw than with alfalfa was due to the fact that straw had a higher percentage of microbially-resistant compounds such al lignin, and lower N content than alfalfa. Greater decomposition rate of alfalfa as compared with wheat straw and liquorice root refuses apparently substantiates this hypothesis that a lower nitrogen content or a wide C:N ratio is associated with lower decomposition, because the C:N ratios of alfalfa, wheat straw, and liquorice root refuse are 10.32:1, 51.21:1, and (25.65 -28.68):1, respectively (Table 4); yet, it is not the sole determinant, and other factors, e.g., chemical composition, especially abundant of lignin, may also be operative.…”