Effect of crop rotation and soil amendments on Sclerotinia stem rot on soybean in two soils

Rousseau, Guillaume Xavier; Rioux, S.; Dostaler, Daniel

doi:10.4141/p05-137

Cited by 24 publications

(18 citation statements)

References 28 publications

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“…In general, the management of S. sclerotiorum borne disease is not much easy because of its widespread existence, irregular incidence, and the long-term survival by producing huge numbers of sclerotia in the soil. Although several control measures like chemical and cultural methods have been devised and adopted for countering the Sclerotinia stem rot menace (Rousseau et al, 2007) none of them were found fully effective in preventing either the process of disease infection or pathogenesis progression after infection. The extent of genetic diversity of the pathogen and their widespread distribution among host species across the growing regions play an important role in determining and devising the control strategies to efficiently control the diseases in the more effective way.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity Studies Based on Morphological Variability, Pathogenicity and Molecular Phylogeny of the Sclerotinia sclerotiorum Population From Indian Mustard (Brassica juncea)

et al. 2018

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White mold or stem rot disease are ubiquitously distributed throughout the world and the causal organism of this disease Sclerotinia sclerotiorum (Lib.) de Bary, is known to infect over 400 plant species. Sclerotinia stem rot is one of the most devastating fungal diseases and poses a serious threat to the worldwide cultivation of oilseed Brassica including India. S. sclerotiorum pathogen usually infects the stem but in severe cases leaves and pods also affected at different developmental stages that deteriorate not only the oil quality but also causing the seed and oil yield losses up to 90% depending on the severity of the disease infestation. This study investigated the morphological and molecular characterization of pathogenic S. sclerotiorum (Lib) de Bary geographical isolates from oilseed Brassica including Brassica juncea (Indian mustard). The aim of this study was to compare isolates of S. sclerotiorum originated from different agro-climatic conditions and to analyse similarity or differences between them as well as to examine the virulence of this pathogen specifically in Brassica for the first time. The collection of S. sclerotiorum isolates from symptomatic Brassica plants was done and analyzed for morphological features, and molecular characterization. The virulence evaluation test of 65 isolates on four Brassica cultivars has shown 5 of them were highly virulent, 46 were virulent and 14 were moderately virulent. Phylogenetic analysis encompassing all the morphological features, SSR polymorphism, and ITS sequencing has shown the existence of high genetic diversity among the isolates that categorized all the isolates in three evolutionary lineages in the derived dendrogram. Further, genetic variability analysis based on sequences variation in ITS region of all the isolates has shown the existence of either insertions or deletions of the nucleotides in the ITS region has led to the interspecies variability and observed the variation were in a clade-specific manner. Together this analysis observed the existence of higher heterogeneity and genetic variability in S. sclerotiorum isolates collection and indicates the presence of clonal and sexual progenies of the pathogen in the mustard growing regions of India surveyed in this study. With a higher level of genetic variability and diversity among the S. sclerotiorum population needs robust screening approaches to identify the donor parent and utilize them in resistance breeding program for effectively counter the menace of stem rot disease in Brassica.

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Section: Introductionmentioning

confidence: 99%

Genetic Diversity Studies Based on Morphological Variability, Pathogenicity and Molecular Phylogeny of the Sclerotinia sclerotiorum Population From Indian Mustard (Brassica juncea)

et al. 2018

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“…However, these products need to be applied yearly to obtain the best efficacy and will be unnecessary in the years where climatic conditions are not favorable for disease development. Cultural practices such as crop rotation (Kurle et al, 2001; Rousseau et al, 2007), reduced tillage (Sutton and Peng, 1993; Gracia‐Garza et al, 2002; Mueller et al, 2002), and wide row spacing (Kurle et al, 2001; Mila and Yang, 2008) have been reported to reduce the impact of SSR in soybean fields. The rationale behind these practices is to decrease inoculum and/or maintain unfavorable conditions for fungal development.…”

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confidence: 99%

Genome Wide Association Mapping of Sclerotinia sclerotiorum Resistance in Soybean with a Genotyping‐by‐Sequencing Approach

2014

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Sclerotinia stem rot (SSR) is one of the most important pests in cool soybean growing regions of the Northeastern United States and Canada. However, the intensity of infestations varies considerably from year to year according to weather conditions, thus making it difficult for breeders to select under uniform disease pressure. Selection for resistance to SSR would be greatly facilitated by the use of molecular markers. In this work, a collection of 130 lines was inoculated using the cotton pad method and was genetically characterized using a genotyping-by-sequencing (GBS) protocol optimized for soybean. Genome-wide association mapping (AM) and linkage disequilibrium (LD) analyses were performed with 7864 single nucleotide polymorphisms (SNPs). Linkage disequilibrium varied considerably over physical distance, reaching a r 2 value of 0.2 after 8.5 Mb in the pericentromeric region and 0.5 Mb in the telomeric region. The mixed linear model (MLM) performed very well in accounting for population structure and relatedness, as only 5.5% of the observed p-values were < 0.05. The strongest association was found on chromosome Gm15 (p-value = 1.38 × 10 -6

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“…However, their economical use on a field crop such as soybean remains to be demonstrated. Although some cultural practices such as crop rotation (Kurle et al, 2001; Mitchell and Wheeler, 1990; Rousseau et al, 2007), reduced tillage (Gracia‐Garza et al, 2002; Mueller et al, 2002; Sutton and Peng, 1993), and large row spacing (Mila and Yang, 2008) have been shown to reduce the severity of the disease, the development of resistant varieties is viewed as the most effective control measure and an environmentally attractive alternative (Grau and Hartman, 1999; Steadman, 1979). It has become an important objective in many breeding programs.…”

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confidence: 99%

Identification of QTLs Associated with Partial Resistance to White Mold in Soybean Using Field‐Based Inoculation

et al. 2010

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White mold caused by Sclerotinia sclerotiorum (Lib.) de Bary can be an important cause of yield loss in soybean. Only partial resistance to this disease has been found to date, and little is known about the loci contributing to this resistance. The objectives of this study were to identify quantitative trait loci (QTL) associated with partial resistance to white mold progression on the main stem. One hundred eighty F4–derived lines from a cross between a partially resistant cultivar, Maple Donovan, and a susceptible cultivar, OAC Bayfield, were tested for resistance to white mold under field conditions with (2003 and 2004) or without irrigation (2005 and 2006). The resistance of the lines was assessed by measuring the length of lesions 7 d after inoculation with a mycelial suspension. These lines were genotyped with 128 simple sequence repeat markers, and three QTLs associated with lesion length were detected consistently (in at least three of the four trials). Two of the QTLs were located on LG C2 (chromosome 6) and the third was on LGI (chromosome 20). In these genomic regions, the favorable alleles came from Maple Donovan and contributed to a decrease in lesion length. Together, they accounted for 30.7 to 50.9% of lesion length variation across year trials. Selective phenotyping of 26 lines carrying contrasting alleles at these QTLs in four additional environments resulted in significant phenotypic contrasts between the two genotypic classes.

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Effect of crop rotation and soil amendments on Sclerotinia stem rot on soybean in two soils

Cited by 24 publications

References 28 publications

Genetic Diversity Studies Based on Morphological Variability, Pathogenicity and Molecular Phylogeny of the Sclerotinia sclerotiorum Population From Indian Mustard (Brassica juncea)

Genetic Diversity Studies Based on Morphological Variability, Pathogenicity and Molecular Phylogeny of the Sclerotinia sclerotiorum Population From Indian Mustard (Brassica juncea)

Genome Wide Association Mapping of Sclerotinia sclerotiorum Resistance in Soybean with a Genotyping‐by‐Sequencing Approach

Identification of QTLs Associated with Partial Resistance to White Mold in Soybean Using Field‐Based Inoculation

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