Effect of crowding stress on growth performance, the antioxidant system and humoral immunity in hybrid sturgeon

Bi, Baoliang; Yin, Yulong; Zhao, Yao; He, Mingyi; Song, Haoqing; Kong, Lingyin; Gao, Yu

doi:10.1016/j.aqrep.2023.101468

Cited by 9 publications

(7 citation statements)

References 50 publications

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“…Another study demonstrated that under crowding stress, immune indicators in hybrid sturgeon (♀Acipenser baerii × ♂Acipenser schrenckii) did not show significant changes. However, pathways related to fatty acid degradation, gluconeogenesis, and glycogen breakdown were significantly activated to account for the increased energy expenditure in sturgeon under crowding stress [69]. Our study found that after the blockade of the glutamate metabolism pathway under carbonate alkaline stress, metabolites closely associated with the fatty acid degradation pathway exhibited a significant decrease.…”

Section: Energy Metabolism Disordermentioning

confidence: 60%

Investigating the Impact of Disrupting the Glutamine Metabolism Pathway on Ammonia Excretion in Crucian Carp (Carassius auratus) under Carbonate Alkaline Stress Using Metabolomics Techniques

Sun,

Geng,

Liu

et al. 2024

Antioxidants

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With the gradual decline in freshwater resources, the space available for freshwater aquaculture is diminishing and the need to maximize saline water for aquaculture is increasing. This study aimed to elucidate the impact mechanisms of the disruption of the glutamate pathway on serum metabolism and ammonia excretion in crucian carp (Carassius auratus) under carbonate alkaline stress. A freshwater control group (C group), a 20 mmol/L NaHCO3 stress group (L group), and a 40 mmol/L NaHCO3 stress group (H group) were established. After 30 days of exposure, methionine sulfoximine (MSO) was injected to block the glutamate pathway metabolism, and the groups post-blocking were labeled as MC, ML, and MH. Ultra-high-performance liquid chromatography coupled with the quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) metabolomics technique was employed to detect changes in the composition and content of crucian carp serum metabolites. Significant differential metabolites were identified, and related metabolic pathways were analyzed. The results revealed that, following the glutamate pathway blockade, a total of 228 differential metabolites (DMs) were identified in the three treatment groups. An enrichment analysis indicated significant involvement in glycerophospholipid metabolism, arachidonic acid metabolism, sphingolipid metabolism, purine metabolism, arginine and proline biosynthesis, pantothenate and CoA biosynthesis, glutathione metabolism, and fatty acid degradation, among other metabolic pathways. The results showed that ROS imbalances and L-arginine accumulation in crucian carp after the glutamate pathway blockade led to an increase in oxidative stress and inflammatory responses in vivo, which may cause damage to the structure and function of cell membranes. Crucian carp improves the body’s antioxidant capacity and regulates cellular homeostasis by activating glutathione metabolism and increasing the concentration of phosphatidylcholine (PC) analogs. Additionally, challenges such as aggravated ammonia excretion obstruction and disrupted energy metabolism were observed in crucian carp, with the upregulation of purine metabolism alleviating ammonia toxicity and maintaining energy homeostasis through pantothenate and CoA biosynthesis as well as fatty acid degradation. This study elucidated the metabolic changes in crucian carp under carbonate alkaline stress after a glutamate pathway blockade at the cellular metabolism level and screened out the key metabolic pathways, which provide a scientific basis for further in-depth studies on the ammonia excretion of freshwater scleractinian fishes under saline and alkaline habitats at a later stage.

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Section: Energy Metabolism Disordermentioning

confidence: 60%

Investigating the Impact of Disrupting the Glutamine Metabolism Pathway on Ammonia Excretion in Crucian Carp (Carassius auratus) under Carbonate Alkaline Stress Using Metabolomics Techniques

Sun,

Geng,

Liu

et al. 2024

Antioxidants

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“…Although several previous studies have reported that overcrowding induces changes in 5-HT [61,62], TNF-a [63], SOD [64], and MDA [65,66] levels, further study with control nonstressed animals are required to provide more convincing evidence on the role of AKE as antidepressant by modulating the brain neurotransmitters, pro-inflammatory cytokines, and antioxidant signaling pathways.…”

Section: Discussionmentioning

confidence: 99%

“…This fact limits the conclusion drawn from the brain neurotransmitter, pro-inflammatory cytokine, and oxidative stress markers, as it cannot be discerned as to whether overcrowding causes changes to these parameters and whether AKE treatments reversed them. Although several previous studies have reported that overcrowding induces changes in 5-HT [61,62], TNF-a [63], SOD [64], and MDA [65,66] levels, further study with control non-stressed animals are required to provide more convincing evidence on the role of AKE as anti-depressant by modulating the brain neurotransmitters, pro-inflammatory cytokines, and antioxidant signaling pathways.…”

Section: Discussionmentioning

confidence: 99%

Angelica keiskeileaves extract attenuates psychosocial stress in overcrowding-subjected rats

Siswanto

2024

Preprint

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Angelica keiskei is currently used as a popular functional food with various beneficial effects, including antioxidant, anti-obesity, anti-tumor, anti-diabetic, and anti-bacterial. A recent in vitro study reported that A. keiskei inhibits monoamine oxidases (MAOs), suggesting the antidepressant property of A. keiskei. However, in vivo studies on laboratory animals subjected to psychosocial stress have not been conducted. In this study, the effects of an A. keiskei leaves extract (AKE) on rats undergoing chronic social overcrowding stress were explored. Six-month-old male and female Wistar rats were housed in groups of three in 12 x 12 x 18 cm cages (overcrowding) for twenty-eight days. Both male and female rats were divided into two groups (N = 10); control group received oral distilled water, whilst the other group (treatment group) received 20 mg/kgBW/day of AKE supplementation. The results showed that AKE-treated rats exhibited lower anxiety- and depressive-related behaviors than that of control stressed rats. AKE significantly decreased corticosterone and increased testosterone and estrogen levels in stressed rats. Additionally, brain tissue MDA and TNF-alpha levels were reduced, while brain neurotransmitter 5-HT and antioxidant SOD levels were elevated by the AKE. These findings suggest, for the first time, that AKE could alleviate overcrowding stress-induced behavioral, neuroendocrine, antioxidant, and inflammatory dysfunctions. A. keiskei leaves extract could potentially be used as an agonist to reduce stress and depression.

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“…Cortisol levels were measured using commercially available enzyme‐linked immunosorbent assay (ELISA) kits with a test range of 50–3000 pg/mL (kit no. : JM‐0030F1, Jiangsu Jingmei Biotechnology Ltd., Yancheng, China) (Bi et al, 2023). Experimental results were measured at 450 nm using a microplate reader (iMark, Bio‐Rad, Hercules, USA).…”

Section: Methodsmentioning

confidence: 99%

Indexing serum and mucous biochemical parameters of endangered Chinese sturgeon Acipenser sinensis with implications for health assessment

Liu,

UllahKhan,

Jin

et al. 2024

Journal of Fish Biology

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The Chinese sturgeon (Acipenser sinensis) is a critically endangered aquatic fish. Health monitoring and welfare assessments are critical for the conservation of Chinese sturgeon. In this study, biochemical parameters of serum and skin mucus in Chinese sturgeon were examined to evaluate the potential biomarkers. Serum and mucous samples were obtained from Chinese sturgeon, and the levels of total protein (TP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), creatine kinase (CK), lactic acid (LD), acid phosphatase (ACP), lysozyme (LYZ), glucose (GLU), and cortisol were determined. The concentrations of ALT, AST, cortisol, and LYZ were significantly higher in the mucous group than those in the serum group (p < 0.05). In addition, the concentrations of ALP, ACP, LD, LDH, CK, and TP were significantly higher level in the serum group than those in the mucous group (p < 0.05). Moreover, the correlations between serum and mucous biochemical parameters were established. Statistical analysis showed a positive correlation between serum and skin mucous markers (ACP, cortisol, and LYZ). AST versus ALT in serum and mucus showed a significant positive correlation (p < 0.01). A significant positive correlation was found between cortisol and CK in mucus (p < 0.01). Moreover, LD versus LDH in serum showed a significant but weak positive correlation (p < 0.01). Principal component analysis revealed a complete separation between the serum and mucous groups, with the biomarkers that contributed the most being ALP, TP, ALT, and AST. This study provides baseline data and reference intervals for serum and mucous biochemical parameters in presumably healthy Chinese sturgeons. The current study has important implications for the development of conservation strategies and the conservation status of critically endangered species.

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Effect of crowding stress on growth performance, the antioxidant system and humoral immunity in hybrid sturgeon

Cited by 9 publications

References 50 publications

Investigating the Impact of Disrupting the Glutamine Metabolism Pathway on Ammonia Excretion in Crucian Carp (Carassius auratus) under Carbonate Alkaline Stress Using Metabolomics Techniques

Investigating the Impact of Disrupting the Glutamine Metabolism Pathway on Ammonia Excretion in Crucian Carp (Carassius auratus) under Carbonate Alkaline Stress Using Metabolomics Techniques

Angelica keiskeileaves extract attenuates psychosocial stress in overcrowding-subjected rats

Indexing serum and mucous biochemical parameters of endangered Chinese sturgeon Acipenser sinensis with implications for health assessment

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