Effect of crude glycerol impurities on lipid preparation by Rhodosporidium toruloides yeast 32489

Gao, Zhen; Ma, Yingqun; Wang, Qunhui; Zhang, Min; Wang, Juan; Liu, Yu

doi:10.1016/j.biortech.2016.06.088

Cited by 83 publications

(37 citation statements)

References 32 publications

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“…Since the lipid obtained was predominantly rich in oleic acid, a type of MUFA, it is considered quite suitable for biodiesel production (Uprety, Dalli, et al, ). Similar fatty acid composition has been reported previously for different strains of R. toruloides (Gao et al, ; Uprety, Dalli, et al, ; Xu et al, ).…”

Section: Resultssupporting

confidence: 89%

Use of Essential Oils From Various Plants to Change the Fatty Acids Profiles of Lipids Obtained From Oleaginous Yeasts

Uprety

Rakshit

2018

J Americ Oil Chem Soc

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We studied the ability of seven essential oils to alter the fatty acid composition of lipids produced by an oleaginous yeast Rhodosporidium toruloides. All of the essential oils, except thyme, significantly increased the stearic acid content of the lipids. The amount of essential oils in the media determined the fatty acid composition obtained. Subsequently, we studied the effect of the major monoterpenes present in these essential oils. When R. toruloides was grown on limonene, a major monoterpene in orange essential oil, the composition of lipid obtained was found to be quite similar to natural orange essential oil. This proved that limonene has a major role in the changes in fatty acid profiles of the lipids. The effect of orange essential oil on another oleaginous yeast, Cryptococcus curvatus, was also carried out. Although the effect of the essential oil on the fatty acid composition and biomass (cell mass) was similar for both these two yeasts, the reduction of the activity of some enzymes involved in the metabolic pathways was quite different. From these results, it can be concluded that the effect of essential oils differs with species and it is possible to produce lipids with alternate fatty acid profiles suitable for different applications and with good market value.

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Section: Resultssupporting

confidence: 89%

Use of Essential Oils From Various Plants to Change the Fatty Acids Profiles of Lipids Obtained From Oleaginous Yeasts

Uprety

Rakshit

2018

J Americ Oil Chem Soc

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“…Nevertheless, the proportion of saturated fatty acids and unsaturated fatty acids were slightly different among the different crude glycerol concentrations. Gao et al [36] reported that no significant difference in fatty acids composition and content of lipids produced by R. toruloides 32489 cultivated in pure glycerol and crude glycerol, indicating that the presence of impurities in crude glycerol did not influence the fatty acid biosynthesis pathway. Consequently, the crude glycerol concentration of 70 g/L was selected for further investigation in two-stage fed-batch cultivation.…”

Section: Effect Of Crude Glycerol Concentration On Growth and Lipid Amentioning

confidence: 99%

Feeding Strategies of Two-Stage Fed-Batch Cultivation Processes for Microbial Lipid Production from Sugarcane Top Hydrolysate and Crude Glycerol by the Oleaginous Red Yeast Rhodosporidiobolus fluvialis

Poontawee

2020

Microorganisms

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Microbial lipids are able to produce from various raw materials including lignocellulosic biomass by the effective oleaginous microorganisms using different cultivation processes. This study aimed to enhance microbial lipid production from the low-cost substrates namely sugarcane top hydrolysate and crude glycerol by Rhodosporidiobolus fluvialis DMKU-SP314, using two-stage fed-batch cultivation with different feeding strategies in a 3 L stirred-tank fermenter. The effect of two feeding strategies of 147.5 g/L crude glycerol solution was evaluated including pulse feeding at different starting time points (48, 24, and 72 h after initiation of batch operation) and constant feeding at different dilution rates (0.012, 0.020, and 0.033 h−1). The maximum lipid concentration of 23.6 g/L and cell mass of 38.5 g/L were achieved when constant feeding was performed at the dilution rate of 0.012 h−1 after 48 h of batch operation, which represented 1.24-fold and 1.27-fold improvements in the lipid and cell mass concentration, respectively. Whereas, batch cultivation provided 19.1 g/L of lipids and 30.3 g/L of cell mass. The overall lipid productivity increased to 98.4 mg/L/d in the two-stage fed-batch cultivation. This demonstrated that the two-stage fed-batch cultivation with constant feeding strategy has the possibility to apply for large-scale production of lipids by yeast.

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“…Lipid accumulation in Kodamaea ohmeri with crude glycerol was only 50% of that with pure glycerol (Kitcha and Cheirsilp, 2011). Gao et al (2016) found that the lipid productivity of Rhodosporidium toruloides in crude glycerol cultivation was reduced around 18% compared with that of pure glycerol cultivation (Gao et al, 2016). Overall, crude glycerol with high glycerol content and low impurity content showed advantage on lipid production from oleaginous microorganism.…”

Section: Introductionmentioning

confidence: 96%

“…It is normally called crude glycerol and mainly contains glycerol (20-96% w/w), free fatty acids, soaps, catalyst, salts, methanol etc. (Gao et al, 2016;Hansen et al, 2009;Hu et al, 2012). The composition of crude glycerol varies from one biodiesel production plant to another and is mainly determined by the feedstock oil composition and quality, the oil and methanol molar ratio used in transesterification, type of catalyst used, and the detailed procedure such as with or without methanol recovery (Athalye et al, 2009;Uprety et al, 2017;Yen et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Chemical and biological conversion of crude glycerol derived from waste cooking oil to biodiesel

et al. 2018

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In this study, crude, purified, and pure glycerol were used to cultivate Trichosporon oleaginosus for lipid production which was then used as feedstock of biodiesel production. The purified glycerol was obtained from crude glycerol by removing soap with addition of HPO which converted soap to free fatty acids and then separated from the solution. The results showed that purified glycerol provided similar performance as pure glycerol in lipid accumulation; however, crude glycerol as carbon source had negatively impacted the lipid production of T. oleaginosus. Purified glycerol was later used to determine the optimal glycerol concentration for lipid production. The highest lipid yield 0.19g/g glycerol was obtained at 50g/L purified glycerol in which the biomass concentration and lipid content were 10.75g/L and 47% w/w, respectively. An energy gain of 4150.51MJ could be obtained with 1tonne of the crude glycerol employed for biodiesel production through the process proposed in this study. The biodiesel production cost estimated was 6.32US$/gal. Fatty acid profiles revealed that C16:0 and C18:1 were the major compounds of the biodiesel from the lipid produced by T. oleaginosus cultivated with crude and purified glycerol. The study found that purified glycerol was promising carbon source for biodiesel production.

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Effect of crude glycerol impurities on lipid preparation by Rhodosporidium toruloides yeast 32489

Cited by 83 publications

References 32 publications

Use of Essential Oils From Various Plants to Change the Fatty Acids Profiles of Lipids Obtained From Oleaginous Yeasts

Use of Essential Oils From Various Plants to Change the Fatty Acids Profiles of Lipids Obtained From Oleaginous Yeasts

Feeding Strategies of Two-Stage Fed-Batch Cultivation Processes for Microbial Lipid Production from Sugarcane Top Hydrolysate and Crude Glycerol by the Oleaginous Red Yeast Rhodosporidiobolus fluvialis

Chemical and biological conversion of crude glycerol derived from waste cooking oil to biodiesel

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