Effect of cryopreservant combinations on the motility and morphology of curimba (Prochilodus lineatus) sperm

Felizardo, V. O.; Mello, R. A.; Murgas, Luís David Solis; Andrade, E. S.; Drumond, Mariana Martins; Rosa, Philippe

doi:10.1016/j.anireprosci.2010.08.020

Cited by 34 publications

(38 citation statements)

References 12 publications

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“…However, to achieve good sperm quality after thawing, various factors, such as a good freezing solution and appropriate cooling rates with appropriate equipment, must be considered. Hence, specific parameters, such as motility and morphology, need to be examined (Felizardo et al, 2010). In addition, the semenand-extender interaction is species-specific: a particular extender may prove effective for freezing the semen of one species but yield poorer post-thaw quality with the semen of other species (Salmito-Vanderley et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Use of glucose or BTS"! combined with DMSO or methylglycol under two different freezing protocols for the cryopreservation of sperm from the common curimatã (Prochilodus brevis)

et al. 2016

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This study evaluated the effect of glucose or Beltsville Thawing Solution (BTS™) combined with dimethyl sulfoxide (DMSO) or methylglycol (MG) under two different freezing protocols on the kinetics and morphology of cryopreserved Prochilodus brevis sperm. The semen samples were diluted using one of four different treatments (glucose+DMSO, glucose+MG, BTS™+DMSO, and BTS™+MG), loaded into 0.25-ml straws and subjected to two different freezing processes (programmed freezing machine and dry shipper). After 10 days, the semen samples were thawed, and the sperm morphology and kinetics were evaluated. The physicochemical parameters of the semen in natura were similar to those observed in other studies of Characiformes, indicating the feasibility of semen cryopreservation. Glucose, when used as a diluent with the cryoprotectant MG (glucose+MG), yielded higher percentages of mobile spermatozoa after freezing in a dry shipper (76.88 ± 4.84%) and in a programmed freezing machine (70.95 ± 1.76%) compared with the combination of glucose and DMSO. Moreover, the glucose+MG treatment yielded a higher sperm velocity (curvilinear velocity: 79.52 ± 2.88 µm s -1 ; straight-line velocity: 45.46 ± 3.01 µm s -1 ; average path velocity: 67.92 ± 3.08 µm s -1 ) than the other studied treatments, and a higher amount of normal sperm (74.56 ± 0.77%) was observed in the semen samples cryopreserved using a programmed freezing machine. The sperm abnormalities observed included a bent tail morphology. Therefore, the use of glucose+MG in combination with either a dry shipper or a programmed freezing machine is recommended for the cryopreservation of P. brevis sperm because these methods yielded high numbers of motile and morphologically normal spermatozoa.

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Section: Introductionmentioning

confidence: 99%

Use of glucose or BTS"! combined with DMSO or methylglycol under two different freezing protocols for the cryopreservation of sperm from the common curimatã (Prochilodus brevis)

et al. 2016

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“…A criopreservação do sêmen tem sido testada para várias espécies como, por exemplo, o dourado Salminus maxillosus (Carolsfeld et al, 2003); a piracanjuba Brycon orbignyanus (Carolsfeld et al, 2003;Maria et al, 2006;Felizardo, 2008); o curimbatá Prochilodus lineatus (Felizardo et al, 2010;Murgas et al, 2007) e a tilápia-nilótica Oreochromis niloticus (Amorim, 2002).…”

Section: Introductionunclassified

Osmolaridade e taxa de diluição na ativação do sêmen criopreservado de Prochilodus lineatus

et al. 2011

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PALAVRAS CHAVE ADICIONAISMotilidade espermática. Peixe. ADDITIONAL KEYWORDSSperm motility. Fish. RESUMOObjetivou-se avaliar a influência de ativadores com diferentes osmolaridades e taxa de diluição na ativação de sêmen criopreservado de curimba (Prochilodus lineatus). Foram utilizados quatro reprodutores machos capturados na Estação de Piscicultura da CEMIG, Itutinga, MG. As amostras de sêmen foram diluídas em duas soluções (DMSO + lactose e metanol + lactose) na proporção de 1:4 e congeladas. Foram avaliadas as taxas (%) e duração (s) da motilidade espermática dos tratamentos. Na ativação foram utilizados ativadores contendo as respectivas osmolaridades: 30, 99, 183 e 293 mOsm nas taxas de diluição de 1:2, 1:4, 1:6, 1:8 sêmen: ativador. A taxa de diluição 1:2 e 1:8 foram estatisticamente significativas para duração da motilidade para o sêmen criopreservado com DMSO. Para a diluição 1:2, o sêmen ativado com o ativador contendo 30 mOsm, apresentou duração de motilidade maior em relação ao sêmen ativado com ativadores com 183 e 293 mOsm. No entanto, para a utilização do ativador com 30 mOsm, se obteve maior duração da motilidade na diluição de 1:2 em relação a diluição de 1:4. Na taxa de diluição 1:6, quando o sêmen foi ativado com o ativador de 30 mOsm, a taxa de motilidade foi superior à apresentada pelo sêmen que foi ativado com 293 mOsm. Na taxa de diluição de 1:8, o uso de ativador contendo 99 mOsm apresentou maior duração de motilidade em relação ao uso do ativador com 293 mOsm. Ativadores contendo altas osmolaridades podem proporcionar menores taxas e duração da motilidade em sêmen de curimba (Prochilodus lineatus) criopreservado com os crioprotetores metanol ou DMSO. SUMMARYThe objective of this work was to evaluate the influence of activators with different osmolarities and dilution rate in the activation of cryopreserved semen of curimba (Prochilodus lineatus). We used four breeding males captured in the Fishfarming Station of CEMIG, Itutinga, MG. Semen samples were diluted in two solutions (DMSO + methanol and lactose + lactose) at 1:4 ratio and frozen. The rates (%) and duration (s) of sperm motility for treatments were assessed. Activators with respective osmolarities: 30, 99, 183 and 293 mOsm at dilution rates of 1:2, 1:4, 1:6, 1:8 (semen: activator) were used. The dilution rates of 1:2 and 1:8 influenced duration of motility in semen cryopreserved with DMSO. For the 1:2 dilution, the semen activated with the activator containing 30 mOsm, presented greater motility duration than semen activated with activators with 183 and 293 mOsm. However, for the activator with 30 mOsm, it was obtained longer motility duration at a dilution of 1:2 compared to 1:4 dilution. At 1:6 dilution rate, when the semen was activated with the activator 30 mOsm, the motility rate was higher than that provided by the semen that was activated with 293 mOsm. At the dilution rate of 1:8, the use of activator containing 99 mOsm showed longer motility duration in relation to use of the activator with 293 mOsm. Activators containin...

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“…Atualmente nas pisciculturas, a principal técnica de sexagem e determinação do estágio de maturação gonadal empregada na reprodução é baseada na visualização de características externas como aumento do volume do abdômen, hiperemia e dilatação do poro urogenital (Felizardo et al, 2010). Tais técnicas, por serem subjetivas, podem variar de acordo com as características de cada espécie ou mesmo de indivíduo, resultando muitas vezes em baixa eficiência reprodutiva nas pisciculturas.…”

Section: Introductionunclassified

Determinação do sexo em catfish de canal (Ictalurus punctatus) utilizando endoscopia

Lima¹,

Murgas²,

Felizardo³

et al. 2011

Rev. Ceres

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Sex determination in channel catfish (Ictalurus punctatus) using endoscopySex identification in fish is an important practice for the development of broodstock management strategies. Endoscopy can be used for sex identification, although in fish, it is still restricted. Therefore, the objective of this study was to validate the use of rigid endoscope via the urogenital pore to determine the sex of the catfish (Ictalurus punctatus afinesque, 1818). The equipment used was a Multi-Purpose Rigid ™ 30º endoscope, with 2.7 mm in diameter and 18 cm in length. We examined 60 fish weighing between 86 and 1032 grams, in which the endoscope was introduced via the genital pore and then immediately euthanized to confirm the sex. Gonadal tissue samples were collected for histological assessment of maturation stage and sex confirmation. The results showed that of the 60 fish examined, 57 had positive sex confirmation by endoscopy, corresponding to 95% of the sample. The results show that it is feasible the use of endoscopy for sex determination in catfish via genital pore.

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Effect of cryopreservant combinations on the motility and morphology of curimba (Prochilodus lineatus) sperm

Cited by 34 publications

References 12 publications

Use of glucose or BTS"! combined with DMSO or methylglycol under two different freezing protocols for the cryopreservation of sperm from the common curimatã (Prochilodus brevis)

Use of glucose or BTS"! combined with DMSO or methylglycol under two different freezing protocols for the cryopreservation of sperm from the common curimatã (Prochilodus brevis)

Osmolaridade e taxa de diluição na ativação do sêmen criopreservado de Prochilodus lineatus

Determinação do sexo em catfish de canal (Ictalurus punctatus) utilizando endoscopia

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