Effect of cryopreservation on therapeutic potential of canine bone marrow derived mesenchymal stem cells augmented mesh scaffold for wound healing in guinea pig

Bharti, Mukesh Kumar; Bhat, Irfan A.; Pandey, Sriti; Shabir, Uffaq; Peer, Bilal Ahmad; Indu, B; Bhat, Abas Rashid; Kumar, G. Sai; Amarpal,; Chandra, Vikash; Sharma, G. Taru

doi:10.1016/j.biopha.2019.109573

Cited by 15 publications

(29 citation statements)

References 32 publications

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“…To develop stem cell-based scaffolds, stem cells need to be first harvested from animals or humans and then loaded into the prepared scaffold. Hence, to avoid repeated harvesting of BMSCs for each treatment and cell loading into scaffolds, cryopreservation is vitally important [ 138 , 139 ]. Recently, an animal study was conducted to compare the efficiency of freshly isolated and cryopreserved BMSCs on wound healing [ 138 ].…”

Section: Application Of Stem Cells In Skin Substitutesmentioning

confidence: 99%

“…Hence, to avoid repeated harvesting of BMSCs for each treatment and cell loading into scaffolds, cryopreservation is vitally important [ 138 , 139 ]. Recently, an animal study was conducted to compare the efficiency of freshly isolated and cryopreserved BMSCs on wound healing [ 138 ]. For this, both fresh and 30-day preserved BMSCs were cultured on monofilament polypropylene scaffolds and used for therapeutic purposes in guinea pigs.…”

Section: Application Of Stem Cells In Skin Substitutesmentioning

confidence: 99%

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Immunomodulation of Skin Repair: Cell-Based Therapeutic Strategies for Skin Replacement (A Comprehensive Review)

et al. 2022

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The immune system has a crucial role in skin wound healing and the application of specific cell-laden immunomodulating biomaterials emerged as a possible treatment option to drive skin tissue regeneration. Cell-laden tissue-engineered skin substitutes have the ability to activate immune pathways, even in the absence of other immune-stimulating signals. In particular, mesenchymal stem cells with their immunomodulatory properties can create a specific immune microenvironment to reduce inflammation, scarring, and support skin regeneration. This review presents an overview of current wound care techniques including skin tissue engineering and biomaterials as a novel and promising approach. We highlight the plasticity and different roles of immune cells, in particular macrophages during various stages of skin wound healing. These aspects are pivotal to promote the regeneration of nonhealing wounds such as ulcers in diabetic patients. We believe that a better understanding of the intrinsic immunomodulatory features of stem cells in implantable skin substitutes will lead to new translational opportunities. This, in turn, will improve skin tissue engineering and regenerative medicine applications.

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Section: Application Of Stem Cells In Skin Substitutesmentioning

confidence: 99%

Section: Application Of Stem Cells In Skin Substitutesmentioning

confidence: 99%

Immunomodulation of Skin Repair: Cell-Based Therapeutic Strategies for Skin Replacement (A Comprehensive Review)

et al. 2022

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“…Polymeric scaffold based bioengineered and synthetic wound dressings are available nowadays in the market. 106 Though such synthetic resources can advance wound healing to some extent, they are expensive to manufacture and can sometimes have an unsatisfactory cosmetic outcome.…”

Section: Homing Property Of Fetal Adnexa Derived Mscsmentioning

confidence: 99%

The preclinical and clinical implications of fetal adnexa derived mesenchymal stromal cells in wound healing therapy

Mankuzhy

Ramesh

Yasotha

et al. 2021

Wound Repair Regeneration

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Mesenchymal stromal cells (MSCs) isolated from fetal adnexa namely amniotic membrane/epithelium, amniotic fluid and umbilical cord have hogged the limelight in recent times, as a proposed alternative to MSCs from conventional sources.These cells which are identified as being in a developmentally primitive state have many advantages, the most important being the non-invasive nature of their isolation procedures, absence of ethical concerns, proliferation potential, differentiation abilities and low immunogenicity. In the present review, we are focusing on the potential preclinical and clinical applications of different cell types of fetal adnexa, in wound healing therapy. We also discuss the isolation-culture methods, cell surface marker expression, multi-lineage differentiation abilities, immunemodulatory capabilities and their homing property. Different mechanisms involved in the wound healing process and the role of stromal cells in therapeutic wound healing are highlighted. Further, we summarize the findings of the cell delivery systems in skin lesion models and paracrine functions of their secretome in the wound healing process. Overall, this holistic review outlines the research findings of fetal adnexa derived MSCs, their usefulness in wound healing therapy in human as well as in veterinary medicine.

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“…However, there are now studies that suggest cryopreservation may not negatively impact the functionality of MSCs [35][36][37][38]. Six studies used mice for their experiments (35,37,39,40,42,50), six studies used rats [36,41,43,46,48,49], one study used both mice and rats [44], one study used beagle dogs [47], and one study used guinea pigs [45]. Nine studies included a 'vehicle only' as an additional control arm [36,37,[39][40][41]45,46,49,50], while four studies employed a sham animal model, where disease negative animals received MSCs or vehicle [42][43][44]48].…”

Section: Introductionmentioning

confidence: 99%

Comparison of freshly cultured versus cryopreserved mesenchymal stem cells in animal models of inflammation: A pre-clinical systematic review

Dave

Mei

McRae

et al. 2022

eLife

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Background:Mesenchymal stem cells (MSCs) are multipotent cells that demonstrate therapeutic potential for the treatment of acute and chronic inflammatory-mediated conditions. Although controversial, some studies suggest that MSCs may lose their functionality with cryopreservation which could render them non-efficacious. Hence, we conducted a systematic review of comparative pre-clinical models of inflammation to determine if there are differences in in vivo measures of pre-clinical efficacy (primary outcomes) and in vitro potency (secondary outcomes) between freshly cultured and cryopreserved MSCs.Methods:A systematic search on OvidMEDLINE, EMBASE, BIOSIS, and Web of Science (until January 13, 2022) was conducted. The primary outcome included measures of in vivo pre-clinical efficacy; secondary outcomes included measures of in vitro MSC potency. Risk of bias was assessed by the SYRCLE ‘Risk of Bias’ assessment tool for pre-clinical studies.Results:Eighteen studies were included. A total of 257 in vivo pre-clinical efficacy experiments represented 101 distinct outcome measures. Of these outcomes, 2.3% (6/257) were significantly different at the 0.05 level or less; 2 favoured freshly cultured and 4 favoured cryopreserved MSCs. A total of 68 in vitro experiments represented 32 different potency measures; 13% (9/68) of the experiments were significantly different at the 0.05 level or less, with seven experiments favouring freshly cultured MSC and two favouring cryopreserved MSCs.Conclusions:The majority of preclinical primary in vivo efficacy and secondary in vitro potency outcomes were not significantly different (p<0.05) between freshly cultured and cryopreserved MSCs. Our systematic summary of the current evidence base may provide MSC basic and clinical research scientists additional rationale for considering a cryopreserved MSC product in their pre-clinical studies and clinical trials as well as help identify research gaps and guide future related research.Funding:Ontario Institute for Regenerative Medicine

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Effect of cryopreservation on therapeutic potential of canine bone marrow derived mesenchymal stem cells augmented mesh scaffold for wound healing in guinea pig

Cited by 15 publications

References 32 publications

Immunomodulation of Skin Repair: Cell-Based Therapeutic Strategies for Skin Replacement (A Comprehensive Review)

Immunomodulation of Skin Repair: Cell-Based Therapeutic Strategies for Skin Replacement (A Comprehensive Review)

The preclinical and clinical implications of fetal adnexa derived mesenchymal stromal cells in wound healing therapy

Comparison of freshly cultured versus cryopreserved mesenchymal stem cells in animal models of inflammation: A pre-clinical systematic review

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