Effect of Cryphonectria hypovirus 1 (CHV1) infection on Cpkk1, a mitogen-activated protein kinase kinase of the filamentous fungus Cryphonectria parasitica

Study of mRNA Expression by Real Time PCR of Cpkk1, Cpkk2 and Cpkk3, three MEKs of Cryphonectria parasitica, in Virus-free and Virus-infected Isogenic Isolates

“…We could show a statistically significant increase in the relative abundance of Cpkk1, Cpkk2 and Cpkk3 mRNAs in virus infected strains when compared to virus free strains only at 4 days postinoculation. Such a result confirms a previous study at the protein expression level where Cpkk1 abundance was altered only at the end of the growth phase (Turina et al. 2006).…”

“…Cpkk1 and Cpkk2, two MEK from C. parasitica, were previously cloned and characterised (Turina et al. 2006).…”

“…It will be necessary to investigate if the presence of the virus induces indirect modifications at post translational level also for the MEK Cpkk2 and Cpkk3; in fact, for Cpkk1 it was already demonstrated that the presence of CHV1 increases the abundance and persistence of its hyperphosphorylated forms (Turina et al. 2006).…”

MAP kinase signalling pathway components and targets conserved between the distantly related plant pathogenic fungi Mycosphaerella graminicola and Magnaporthe grisea

“…The predicted MgSte7p is a 459 amino protein with a serine/threonine protein kinase domain (PFAM ID: PF00069) located between amino acids 66-331. The best match to a known protein was to the mitogen-activated protein kinase kinase 2 of Cryphonectria parasitica (Turina et al, 2006) which has 68% of amino acids in common with MgSte7p. The closest match to a predicted protein was to the predicted mitogen-activated protein kinase kinase Bek1p of Blumeria graminis which has 67% of amino acids in common with MgSte7p.…”

Large-scale expressed sequence tag analysis for the chestnut blight fungus Cryphonectria parasitica

“…For example, hypovirus infection was reported to reduce phosphorylation levels of CpMK1, a homologue of M. grisea HOG1 MAPK, in response to hyperosmotic stress (Park et al, 2004). Turina et al (Turina et al, 2006) recently reported that hypovirus infection also altered the phosphorylation state of the MAPKK Cpkk1.…”