Effect of cultivation mode on the production of docosahexaenoic acid by Tisochrysis lutea

Hu, Hao; Ma, Linlin; Shen, Xiao‐Fei; Li, Jiayun; Wang, Hou‐Feng; Zeng, Raymond J.

doi:10.1186/s13568-018-0580-9

Cited by 21 publications

(9 citation statements)

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“…In addition, turbulent mixing by agitation can make some CO 2 in the air dissolve into the medium, thus, the middle agitation speeds might give appropriate CO 2 concentrations in the medium, which could lead to higher lipid (TFAs) content. 52 The biomass concentration also reached the maximum in the middle agitation speeds (150-300 rpm) as mentioned earlier [Fig. 2(A)].…”

Section: Formation Of the Lc-pufassupporting

confidence: 78%

Evaluation of the effect of agitation speed on the growth and high‐value LC‐PUFA formation of Porphyridium cruentum based on basic rheological analysis

Wang

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND: The selection of hydrodynamic properties of microalgae such as agitation speed is usually done according to empirical values without a technical basis, and the selected agitation speeds are not consistent with each other in many references. In this study, we aimed to provide a possible technical basis for the setup of the agitation speed of microalgae suspensions based on basic rheological analysis, in terms of viscosity of microalgae suspensions at different agitation speeds, using the red microalga Porphyridium cruentum as a model. RESULTS:The agitation speed of 150 rpm was optimal for P. cruentum cultivation, with its biomass concentration increased by about 19-57% compared with the microalgae cultured at other agitation speeds. Meanwhile, the content of linoleic acid, arachidonic acid and total fatty acids increased by maximum 103%, 58% and 48%, respectively, compared with those at other agitation speeds. Furthermore, a low rotational speed was beneficial for eicosapentaenoic acid formation (which increased by 72-106%). CONCLUSION: Basic rheological analysis of microalgal suspensions can be used as a guide in setting a reasonable and suitable agitation speed. Meanwhile, the agitation speed could affect both the biomass growth and the formation of certain high-value products of microalgae.

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Section: Formation Of the Lc-pufassupporting

confidence: 78%

Evaluation of the effect of agitation speed on the growth and high‐value LC‐PUFA formation of Porphyridium cruentum based on basic rheological analysis

Wang

et al. 2019

J of Chemical Tech & Biotech

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“…( Talebi et al, 2013 ), P. tricornutum ( Yongmanitchai and Ward, 1991 ) and Chaetoceros muelleri ( Gao et al, 2013 ), green algae like C. reinhardtii ( James et al, 2011 ), Chlorella minutissima ( Vazhappilly and Chen, 1998 ), Dunaliella salina ( Bhosale et al, 2010 ), and Scenedesmus sp. ( Talebi et al, 2013 ) as well as Haptophyta such as I. galbana ( Tzovenis et al, 1997 ), Pavlova lutheri ( Guihéneuf et al, 2009 ), and Tisochrysis lutea ( Hu et al, 2018 ). In contrast, heterotrophic species such as the dinoflagellate Crypthecodinium cohnii ( Mendes et al, 2009 ) and thraustochytrids can accumulate greater proportions of DHA compared to EPA ( Table 1 ).…”

Section: Microalgal Fatty Acidsmentioning

confidence: 99%

Fatty Acids Derivatives From Eukaryotic Microalgae, Pathways and Potential Applications

Blasio

Balzano

2021

Front. Microbiol.

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The exploitation of petrochemical hydrocarbons is compromising ecosystem and human health and biotechnological research is increasingly focusing on sustainable materials from plants and, to a lesser extent, microalgae. Fatty acid derivatives include, among others, oxylipins, hydroxy fatty acids, diols, alkenones, and wax esters. They can occur as storage lipids or cell wall components and possess, in some cases, striking cosmeceutical, pharmaceutical, and nutraceutical properties. In addition, long chain (>20) fatty acid derivatives mostly contain highly reduced methylenic carbons and exhibit a combustion enthalpy higher than that of C14–20 fatty acids, being potentially suitable as biofuel candidates. Finally, being the building blocks of cell wall components, some fatty acid derivatives might also be used as starters for the industrial synthesis of different polymers. Within this context, microalgae can be a promising source of fatty acid derivatives and, in contrast with terrestrial plants, do not require arable land neither clean water for their growth. Microalgal mass culturing for the extraction and the exploitation of fatty acid derivatives, along with products that are relevant in nutraceutics (e.g., polyunsaturated fatty acids), might contribute in increasing the viability of microalgal biotechnologies. This review explores fatty acids derivatives from microalgae with applications in the field of renewable energies, biomaterials and pharmaceuticals. Nannochloropsis spp. (Eustigmatophyceae, Heterokontophyta) are particularly interesting for biotechnological applications since they grow at faster rates than many other species and possess hydroxy fatty acids and aliphatic cell wall polymers.

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“…Copyright Some green microalgae, like Trebouxiophytes, are known to assimilate a variety of carbon molecules for growth, and this is thought to have arisen from their long evolutionary history as members of lichens 46 . While chlorophytes like Chlamydomonas can use acetate for growth, the brown marine microalga Tisochrysis lutea, known for its potential to commercially produce very-long-chain polyunsaturated fatty acids (VLC-PUFAs), cannot use acetate but can use glycerol for growth 47 . Biomass concentration of more than 100 g l −1 dry cell weight has been achieved with Chlorella with optimized addition of organic carbon sources in a fed-batch mode 48 .…”

Section: Discussionmentioning

confidence: 99%

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

Alzahmi

Daakour

Assal

et al. 2021

JoVE

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Metabolic models are reconstructed based on an organism's available genome annotation and provide predictive tools to study metabolic processes at a systemslevel. Genome-scale metabolic models may include gaps as well as reactions that are unverified experimentally. Reconstructed models of newly isolated microalgal species will result in weaknesses due to these gaps, as there is usually sparse biochemical evidence available for the metabolism of such isolates. The phenotype microarray (PM) technology is an effective, high-throughput method that functionally determines cellular metabolic activities in response to a wide array of entry metabolites. Combining the high throughput phenotypic assays with metabolic modeling can allow existing metabolic network models to be rapidly reconstructed or optimized by providing biochemical evidence to support and expand genomic evidence. This work will show the use of PM assays for the study of microalgae by using the green microalgal model species Chlamydomonas reinhardtii as an example. Experimental evidence for over 254 reactions obtained by PM was used in this study to expand and refine a genome-scale C. reinhardtii metabolic network model, iRC1080, by approximately 25 percent. The protocol created here can be used as a basis for functionally profiling the metabolism of other microalgae, including known microalgae mutants and new isolates.network models can guide the rational designs for the rapid development of optimization strategies 1 , 2 , 3 , 4 .Although approximately 160 microalgal species have been sequenced 5 , there are, to our knowledge, only 44 algal

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Effect of cultivation mode on the production of docosahexaenoic acid by Tisochrysis lutea

Cited by 21 publications

References 50 publications

Evaluation of the effect of agitation speed on the growth and high‐value LC‐PUFA formation of Porphyridium cruentum based on basic rheological analysis

Evaluation of the effect of agitation speed on the growth and high‐value LC‐PUFA formation of Porphyridium cruentum based on basic rheological analysis

Fatty Acids Derivatives From Eukaryotic Microalgae, Pathways and Potential Applications

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

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