Effect of culture conditions on lipase production by Fusarium solani in batch fermentation

Maia, Maria Leonor Alves; Heasley, A; Morais, Márcia Maria Camargo de; Melo, E. H. M.; Morais, Marcos Antônio de; Ledingham, W. M.; Filho, J. L. Lima

doi:10.1016/s0960-8524(00)00079-1

Cited by 129 publications

(67 citation statements)

References 12 publications

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“…Different incubation periods are required by different fungi for optimum lipase production. Similar incubation time of 4 days was reported for maximum lipase activity in Fusarium solani FS1 by Maia et al [43], in Rhizopus arrhizus by Yang et al [44], in Penicillium notatum by Rehman et al [45] , in Ganoderma lucidum by Amin et al [46] and in Emericella nidulans NFCCI 3643 by Lanka et al [25]. However, an incubation time of 3 days was reported for maximal lipase production in Rhizopus chinensis [47] and Aspergillus niger MTCC 2594 [48].…”

Section: Resultssupporting

confidence: 85%

Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from the Contaminated Soil of a Palm Oil Processing Shed

Ayinla¹,

Ademakinwa²,

Agboola³

2017

J App Biol Biotech

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This study investigated the screening, production and optimization of an extracellular lipase from a fungus isolated from the contaminated soil of a palm oil processing shed. This was with a view to obtaining a strain that can secrete lipase with biochemical properties exploitable for biotechnological applications such as bioremediation of oil contaminated sites. Soil samples were collected from palm oil contaminated sites in Gbogan, Osun State, Nigeria (Latitude N 7°29.1481´ and Longitude E 4°20.7587´). The isolated fungal strains were screened on tributyrin agar for exogenous lipolytic activity. Molecular identification was carried out by amplifications of ITS-1, 5.8S and ITS-2 regions. The effects of incubation time, inducers, pH, temperature, carbon and nitrogen sources were varied for optimal lipase production using one factor at a time approach. Rhizopus oryzae ZAC3 (NCBI accession No: KX035094) was identified as the highest lipaseproducing strain. Maximum lipase production was observed on the fourth day, pH 5.0 and a temperature of 45 o C. Olive oil, xylose and yeast extract were the best inducer, carbon and nitrogen sources respectively for lipase production. There was a 2.02 fold increase in lipase production under these optimized conditions. In conclusion, Rhizopus oryzae ZAC3 lipase has properties exploitable for industrial and biotechnological applications.

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Section: Resultssupporting

confidence: 85%

Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from the Contaminated Soil of a Palm Oil Processing Shed

Ayinla¹,

Ademakinwa²,

Agboola³

2017

J App Biol Biotech

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“…A velocidade de crescimento radial foi calculada através da regressão linear dos dados de raio das colônias versus tempo, segundo a Equação 1. Os fungos filamentosos são considerados bons produtores de enzimas (MAIA et al, 2001), e as lipases fúngicas são as preferidas para aplicação industrial (MAHADIK et al, 2002).…”

Section: Seleção De Fungos Produtores De Lipasesunclassified

“…O fungo E 20 foi classificado como do gênero Penicillium, enquanto que os fungos E 9 e E 21 foram pertencentes ao gênero Aspergillus, sendo ambos reportados como bons produtores de lipases por Carvalho et al (2005) Maia et al (2001).…”

Section: Determinação Da Atividade Lipolíticaunclassified

Avaliação da produção de lipases por diferentes cepas de microrganismos isolados em efluentes de laticínios por fermentação submersa

Roveda

Hemkemeier

Colla

2010

Ciênc. Tecnol. Aliment.

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“…These results were confirmed with Destain et al (2005) who reported that olive oil in combination with glucose was used as the carbon source and inducer for the production of lipase. Also Maia et al (2001) and Li et al (2005) concluded that the production of microbial lipases generally needs a fat-related carbon source. In attempts to optimize the concentration of both glucose and olive oil show that increasing glucose and olive oil concentration up to 1% gave maximum lipolytic activity 556.0 U/mL (Figure 3 and 4).…”

Section: Optimization Of Lipase Production By M Racemosusmentioning

confidence: 99%

Optimization of lipase synthesis by Mucor racemosus - Production in a triple impeller bioreactor

Nadia¹,

Nehad²,

El-Sayed³

et al. 2010

MJM

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Fungal cultures were screened for their ability to produce extracellular lipase through different incubation periods, in spite of some Aspergillus niger gave highest lipolytic activities, Mucor racemosus was selected in this study due to its non-pathogenicity. Optimum lipase activity was obtained after 3 days incubation period at 35 °C using submerged culture technique. Using a modified complex medium (3% peptone; 0.2% KH2PO4; 0.05% KCl; 0.05% MgSO4 . 7H2O; 1% glucose; 1% olive oil and 0.01 M barium acetate as additives; pH was adjusted to 7.0 using 0.2 M phosphate buffer) stimulated M. racemosus lipase by 114.3%. The production of lipolytic enzyme by M. racemosus was up-scaled to 5 L stirred-tank bioreactor. Under optimized conditions, lipase and specific enzyme activities were improved by 2 folds (1211 U/mL and 28 U/mg protein) as compared to shake flask studies.

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Effect of culture conditions on lipase production by Fusarium solani in batch fermentation

Cited by 129 publications

References 12 publications

Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from the Contaminated Soil of a Palm Oil Processing Shed

Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from the Contaminated Soil of a Palm Oil Processing Shed

Avaliação da produção de lipases por diferentes cepas de microrganismos isolados em efluentes de laticínios por fermentação submersa

Optimization of lipase synthesis by Mucor racemosus - Production in a triple impeller bioreactor

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