Effect of culture media and silver nitrate concentration on nanoparticle biosynthesis by a filamentous fungus

Abstract

Recently the demand in the development of eco-friendly nanoparticles as alternative to chemical and physical methods has been increasing so the aim of this study was to evaluate the effect of silver nitrate concentration and extracellular filtrate (EF) produced by a filamentous fungus isolated from a spent catalyst and coded e identified as Penicillium purpurogenum CATMC-AH-1 on Silver nanoparticles (AgNPs) production. The filamentous fungus was growth in two culture media named Sucrose and Czapeck media to produce biomass and its was put in contact with water to get two different extracellular filtrates called EFS (extracellular filtrate sucrose) and EFC (extracellular filtrate Czapeck), the EF has the molecules involved to synthesis and stabilization of AgNPs. Three concentrations of AgNO3 1, 1.5 and 2 mM and both extracellular filtrates were used to produce AgNPs. The AgNPs produced were monitored by UV-visible absorption spectra from 200 to 800 nm while their morphology and size were identified by Transmission Electron Microscopy (TEM) and software analysis SPIP 2.6.0. Results showed that both extracellular filtrates had the ability to produce AgNPs with the three concentrations of AgNO3 used. TEM analysis showed AgNPs with spherical morphology in all systems. The AgNPs synthesized in EFS with the three AgNO3 concentrations showed average sizes of 8.9, 8.4 and 6.7 nm respectively. From EFC, the average sizes of AgNPs were of 14.9, 11.5 and 10.1 nm respectively. In summary, in EFS smallest sizes and diameter dispersion of AgNPs were obtained, comparing to EFC and the spherical shape was similar in both filtrates. The AgNO3 concentration had a positive effect when the EFC filtrate was used since a direct correlation was observed between the concentrations of silver nitrate and increase the absorption band around 420 nm as result of surface plasmon resonance of AgNPs produced. The AgNPs biosynthesized from both EF (EFS and EFC) could be used as antimicrobial agent by their small size. Parameter as silver nitrate concentrations and culture media are important because could be affect the size and concentrations of AgNPs biosynthesized.

Biosynthesis of iron nanoparticles (FeNPs) by Alternaria alternata MVSS-AH-5

“…For the extracellular fungal synthesis of nanoparticles, previously fungal biomass is get in stress by putting it in carbon starvation, in this condition the release of biomolecules its promoted toward the aqueous medium, where nanoparticles biosynthesis is carry out. The type and amount of excreted molecules can be influenced by the nature of the culture media where the fungal mycelium was produced, since the activation of the metabolic pathway required for the utilization of the nutrients present in the culture media depends on this (Alamilla-Martínez et al, 2018). During AgNPs biosynthesis by two extracellular filtrate from Penicillium purpurogenum CATMC-AH-1, in EF-Sucrose smallest sizes, and diameter dispersion of AgNPs was obtained, comparing to EF-Czapek.…”

“…All micrographs were analyzed by using the software SPIP 6.0.2. Nine fields were analyzed per sample and about 150 particles were analyzed (Alamilla-Martínez et al, 2018).…”

Effect of the AuNPs Biosynthesized Used as Anodes on the Performance of a Human Blood Paper-Based Microfluidic Fuel Cell

“…All the ECS Transactions, 100 (1) 109-116 (2021) chemicals used were reagent grade. The synthesis AuNPs was based on the reported in previous work [16].…”

“…In both cases, is a wide possibility to use other types of catalysts. In this sense, biosynthesis have been reported as those that are easy to apply and friendly to the environment [16][17]. The use of catalysts synthesized through biosynthesis in this type of micro fuel cells would add value to this technology.…”