Effect of culture media and conditions on polyunsaturated fatty acids production by Mortierella alpina

Jang, Hung-Der; Lin, Yung‐Ya; Yang, Shang-Shyng

doi:10.1016/j.biortech.2004.12.027

Cited by 91 publications

(84 citation statements)

References 27 publications

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“…Several studies focused on determining the optimal composition of cultural media for oleaginous fungi with the aim to optimize the performance of lipid-producing bioprocesses. The effect of the C/N ratio on lipid metabolism has been investigated for a number of oleaginous yeasts and molds, such as Y. lipolytica and many oleaginous species of Rhodotorula, Candida, Apiotrichum/Cryptococcus, Mortierella (Hall & Ratledge, 1977;Papanikolau et al, 2003;Granger et al, 1992;Wu et al, 2010;Park et al, 1990;Jang et al, 2005;Amaretti et al, 2010), and has been mathematically modeled for some of these www.intechopen.com organisms (Ykema et al, 1986;Granger et al, 1993;Economou et al, 2011). Y. lipolytica is the oleaginous microorganism for which information about the metabolic response to different C/N ratios is most abundant (Beopoulos et al, 2009a), particularly due to the availability of molecular tools for genetic engineering of this organism.…”

Section: Cultivation Condition Of Oleaginous Yeastsmentioning

confidence: 99%

Getting Lipids for Biodiesel Production from Oleaginous Fungi

Rossi¹,

Amaretti²,

Raimondi³

et al. 2011

Biodiesel - Feedstocks and Processing Technologies

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Section: Cultivation Condition Of Oleaginous Yeastsmentioning

confidence: 99%

Getting Lipids for Biodiesel Production from Oleaginous Fungi

Rossi¹,

Amaretti²,

Raimondi³

et al. 2011

Biodiesel - Feedstocks and Processing Technologies

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“…Another interesting observation was, during the exponential growth condition, the lipid profi les were showing more on saturated form than unsaturated whereas in older culture it was more on unsaturated form than saturated form. Hence we concluded that the degree of unsaturation in the fatty acid composition is known to infl uence by low temperature [15]. Further growth in low temperature not only infl uences the PUFAs production but also alters the cell morphology [16].…”

Section: Resultsmentioning

confidence: 78%

Differential temperature effect on the production of enhanced gamma linolenic acid in Mucor rouxii CFR-G15

Mamatha

Venkateswaran

2010

Indian J Microbiol

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Mucor rouxii CFR-G15, a locally isolated phycomycetous fungus, on cultivation at room temperature produced more than 30% (w/w) lipid in their dry cell weight, in which 14.2% accounted to be GLA content of the total fatty acids. It was observed that when incubation temperature lowered at 14°C, GLA content of the mycelium increased signifi cantly (P<0.05) from 14.2% to 21.97%. In order to optimize the cultural conditions for high biomass and lipid production with high GLA content, the fungus was grown in association of two different temperatures and supply of additional glucose in culture medium. Maximum lipid and GLA were obtained 23.56 and 19.5% respectively, when the culture was grown at 28°C for four days and followed by addition of glucose (5%), and lowered the incubation temperature to 14ºC for another four days. The presence of GLA in the oil obtained from M. rouxii CFR-G15 was confi rmed by the gas chromatography-mass spectrometry. Gamma linolenic acid (GLA, n-6) is gaining importance in pharmaceutical and nutraceutical industries because of clinical evidence demonstrated that it has various benefi cial effects in human health. In this paper temperature played a major role in enhancing the GLA content which has been described.

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“…ARA 5.3 gl -1 , where glucose is a carbon source and yeast extract a nitrogen source (Jang et al, 2005). Zhu et al (2003) described a method for ARA production by M. alpina on glucose/defatted soybean meal and sodium nitrate with the yield of 1.87 gl -1 ARA (17.3% of total lipids) from 31.2 g biomass in 7 days.…”

Section: Discussionmentioning

confidence: 99%

Isolation and identification of native lower fungi for polyunsaturated fatty acid (PUFA) production in Thailand, and the effect of carbon and nitrogen sources on growth and production

Deelai¹,

Suetrong²,

Damrianant³

et al. 2015

Afr. J. Biotechnol.

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This research focuses on isolation and identification of polyunsaturated fatty acid (PUFA) producing fungi from natural sources of Thailand, followed by experimental designs for carbon and nitrogen utilizations of the potential strains. The results show that 8 and 16 isolates of lower fungi from freshwater and wet-fallen leaves, respectively, could produce PUFAs. Among these isolates, the NR06 that was isolated from wet-fallen leaves at Tad Ta Phu waterfall, Nakhon Ratchasrima province. They showed the highest level productions of both biomass (15.49±0.24 gl -1 ) and total fatty acids (16.44±0.30%). The major essential fatty acid composition was found to be arachidonic acid (ARA; C20:4n6) (32.24±0.35%). The minors were linoleic acid (C18:2n6) (8.26±0.59%) and γ-linoleic acid (GLA; C18:3n6) (5.48±0.08%). Besides the morphological characterization, taxonomic identification by the 636 bp-ITS region sequencing and phylogenetic analysis were performed. It was demonstrated that the fungal isolate NR06 was classified in the closest species of Mortierella elongata with 99% similarity (GenBank accession no. KF181625). Statistically based experimental designs (Design Expert 7.1 software) were applied to the optimization of biomass and fatty acid production in the NR06 culture. Using the glucose as a carbon source, there was a significant effect on both biomass and ARA production (p-values < 0.0001), whereas using yeast extract as a nitrogen source affected only ARA production (p-value = 0.02). It was suggested that the NR06 isolate could be further optimized for an improvement as a potential ARA producing strain.

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Effect of culture media and conditions on polyunsaturated fatty acids production by Mortierella alpina

Cited by 91 publications

References 27 publications

Getting Lipids for Biodiesel Production from Oleaginous Fungi

Getting Lipids for Biodiesel Production from Oleaginous Fungi

Differential temperature effect on the production of enhanced gamma linolenic acid in Mucor rouxii CFR-G15

Isolation and identification of native lower fungi for polyunsaturated fatty acid (PUFA) production in Thailand, and the effect of carbon and nitrogen sources on growth and production

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