Effect of Culture Medium in Callogenesis from Adult Walnut Leaves (Juglans regia L.)

Abstract

To define preliminary aspects of walnut (Juglans regia L.) callogenesis in indirect propagation systems, this work analyzed callogenic induction in adult leaves of walnut in broadleaf trees (BTM), Murashige and Skoog (MS) Driver Kuniyuki Walnut (DKW) and Woody Plant (WPM) media. Leaf segments of 1 cm 2 , previously sterilized, were placed in each culture medium, supplemented with 21.4 µM naphtalene acetic acid (NAA) and 8.8 µM 6-benzylaminopurine (BAP). A completely random design was used; the experimental unit was a Petri dish with six callus explants. Each treatment was composed of seven Petri dishes. Callogenesis (%), nodular calli (%) and histology of calli explants were evaluated at 4 weeks by staining 10 µm slices with safranine-fastgreen. There were no significant differences (α = 0.05) in callogenesis among evaluated media; but nodular calli percentages were significantly higher in BTM (75%) and WPM (63%). Only in calli obtained in the BTM medium did the histology show the presence of meristematic zones, but without external expression. These results indicate that the medium influences the morphogenic characteristics of resultant callus. Calli produced in BTM presented better morphogenic potential, with meristems at primary stages of organization.

The Effect of Explant Type and 6-Benzyl Adenine (BAP) In Sapodilla (<i>Achras zapota</i>) Micropropagation

“…Those nutrients are likely to be the factor which influence the responses of explants towards the formation of callus (Osman et al, 2016). In the present study, the effects of using WPM medium, in bay leaf tree (Cinnamomum tamala) by Sharma and Nautiyal (2009) reported that almost all explants and plant growth regulator treatments produced compact callus and also by Avil´es et al (2009) in Juglans regia L even in endosperm explant of Barringtonia racemosa L by Osman et al (2016) Non embryogenic callus generally has lost regeneration capacity. But the present investigation reveals that non embryogenic callus can be turned into embryos and plantlets if cultured on appropriate medium.…”

Influencia de las auxinas sobre el enraizamiento in vitro de microtallos de Nothofagus glauca (Phil.) Krasser

“…Baillon y Castanea sativa Mill. (Ríos et al 2005, Avilés et al 2009, Latsague et al 2009. No obstante, cabe destacar que los altos niveles de supervivencia obtenidos pueden ser un indicador de la calidad inicial de los microtallos utilizados en este ensayo, ya que es importante considerar que, además de los contenidos hormonales, las características anatómicas y fisiológicas de las plantas micropropagadas se hacen necesarias para su supervivencia tanto en condiciones in vitro como ex vitro (Hartmann et al 2002, Ríos et al 2005.…”

Development and extension of walnut propagation in Iran

“…Micropropagation can be used for large-scale multiplication of cultivars with desirable traits and the production of healthy and disease-free plants. The micropropagation of J. regia is possible via the cultivation of nodal segments, cotyledon, immature cotyledons, ovules, mature embryos and meristems (Aly et al, 1992;Pijut, 1993a, b;Long et al, 1995;Vahdati et al, 2004;Avıĺes et al, 2009). Progress in developing methods for micro-propagation of J. regia has been very successful over the past decade.…”