EFFECT OF CULTURE pH ON THE GROWTH AND BIOTIN REQUIREMENT IN A STRAIN OF LIPOMYCES STARKEYI

Uzuka, Yasuyuki; Naganuma, Takafumi; Tanaka, Kentaro; Odagiri, Yoko

doi:10.2323/jgam.20.197

Cited by 13 publications

(16 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Influence of biotin in the growth medium on the capacity for biotin uptake Lipomyces starkeyi exhibited normal growth profiles in simple glucose-mineral media with an initial pH ranging from 2.6 to 5.5 (2). This indicates that the yeast Fig.…”

Section: Effect Of Culture Age On Biotin Uptakementioning

confidence: 86%

“…The organism used throughout this work was Lipomyces starkeyi JAM 4753, the same strain employed in the previous work (2,3,5). L. starkeyi IAM 4753 was grown aerobically at 30° (2) on the minimal medium of WICKERHAM (6).…”

Section: Methodsmentioning

confidence: 99%

“…Some studies were performed with a bufierized medium, which was described in the previous paper (2). The cells were harvested at an optical density of approximately 1.0 at 660 nm, washed twice with distilled water, and resuspended in distilled water to an optical density of 1.00 at 660 nm.…”

Section: Methodsmentioning

confidence: 99%

“…During the course of studies on the nutritional requirement of L, starkeyi JAM 4753, we have demonstrated that the biotin-requiring property of the strain depends on the initial pH value of the medium (2). More recent work (3) from our laboratory has presented an evidence that biotin biosynthesis in L, starkeyi JAM 4753 is initiated when the pH value of the medium falls below 5.5.…”

mentioning

confidence: 88%

“…More recent work (3) from our laboratory has presented an evidence that biotin biosynthesis in L, starkeyi JAM 4753 is initiated when the pH value of the medium falls below 5.5. On the other hand, when the strain was grown in a glucose-mineral medium supplemented with biotin, the strain exhibited) a normal growth profile, even in a medium with a pH of 6.5 (2). As a result of this, it is worthwhile to consider whether the biotin transport system of the strain might be active even at a pH of 6.5.…”

mentioning

confidence: 99%

See 4 more Smart Citations

General Properties of Biotin Uptake System in Lipomyces Starkeyi

Uzuka

Naganuma

Tanaka

1975

J. Gen. Appl. Microbiol.

Self Cite

View full text Add to dashboard Cite

Section: Effect Of Culture Age On Biotin Uptakementioning

confidence: 86%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 88%

mentioning

confidence: 99%

See 3 more Smart Citations

General Properties of Biotin Uptake System in Lipomyces Starkeyi

Uzuka

Naganuma

Tanaka

1975

J. Gen. Appl. Microbiol.

Self Cite

View full text Add to dashboard Cite

Differences in enzyme activities of Lipomyces starkeyi between cells accumulating lipid and proliferating cells

Naganuma

Uzuka

Tanaka

et al. 1987

J. Basic Microbiol.

View full text Add to dashboard Cite

Differences in intracellular enzyme activities between cells accumulating lipid and proliferating cells were studied with Lipomyces starkeyi, a fat-producing yeast. When the cells were removed from a medium for lipid accumulation t o a medium for cell proliferation, activities of several enzymes on the glycolytic pathway, pentosephosphate cycle, glycerol 3-phosphate pathway, tricarboxylic acid cycle and fatty-acid biosynthesis pathway changed. In the proliferating cells, activities of glycerol-3-phosphate dehydrogenase (GDH) and ATP citrate lyase were very low, as compared to those in the cells accumulating lipids. Activities of these two enzymes and B-phospho- Further experiments provided a simple control method for the capabilities of the proliferation and lipid accumulation of L. sturkeyi (NAGANUMA et al. 1985a). Development of these methods enabled us to prove the relationship between the changes in capabilities of the yeast and intracellular enzyme activities. I n this report, differences of certain enzyme activities on the glycolytic pathway, pentosephosphate cycle, glycerol 3-phosphate pathway, trirarboxylic acid cycle and fatty-acid biosynthesis pathway in the proliferating cells and the cells accumulating lipid were studied. I n addition, the effects of zinc and manganese ions on enzyme activities were investigated. >laterials and methodsStrain and growth conditions: Lipomyces starkeyi IAM 4753 was grown at 29.5 "C, using a reciprocal shaker as described previously (UZUKA et al. 1974). The yeast was initially grown in a basal medium. As the basal medium, LS medium (glusose: 100 g/l) for enhancing lipid accumulation was used (NAGANUMA et al. 1985a). At 90 hr, the culture was divided into two parts and one was continuously shaken. I n another culture, 0.23 mM zinc ion (with ZnSO, . 7 H,O) and 0.40 mM manganese ion (with MnCI, . 4 H,O) were added to the basal medium to start proliferation.

show abstract

Production of dextranase byLipomyces starkeyi

Koenig

Day

1988

Biotechnol Lett

View full text Add to dashboard Cite

EFFECT OF CULTURE pH ON THE GROWTH AND BIOTIN REQUIREMENT IN A STRAIN OF LIPOMYCES STARKEYI

Cited by 13 publications

References 0 publications

General Properties of Biotin Uptake System in Lipomyces Starkeyi

General Properties of Biotin Uptake System in Lipomyces Starkeyi

Differences in enzyme activities of Lipomyces starkeyi between cells accumulating lipid and proliferating cells

Production of dextranase byLipomyces starkeyi

Contact Info

Product

Resources

About