Effect of Curcumin Against Oxidation of Biomolecules by Hydroxyl Radicals

Borra, Sai Krishna; Mahendra, Jaideep; Gurumurthy, Prema; Jayamathi,; Iqbal, Shabeer S; Mahendra, Little

doi:10.7860/jcdr/2014/8517.4967

Cited by 49 publications

(38 citation statements)

References 48 publications

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“…The cells of colonic epithelium must sense and respond appropriately to the constant immunological challenge of the colonic luminal contents and, at the same time, needs to allow absorption of water, nutrients and molecules important for cellular energy metabolism 21 . Intercellular adhesion in colonic mucosa is maintained by TJs, AJs and, gap junctions.…”

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confidence: 99%

Claudin-3 and occludin content in the glands of colonic mucosa devoid from fecal stream submitted to topical intervention with oil extract of Curcuma longa

et al. 2017

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1 8-Experimental SurgeryActa Cir Bras. 2017;32(1):65-73 AbstractPurpose: To evaluate the inflammatory intensity and measure the tissue content of the proteins claudin-3 and occludin in the colonic mucosa without fecal stream submit to intervention with curcumin. Methods: Thirty-six rats were submitted to a proximal colostomy and a distal mucous fistula and divided into two groups according to sacrifice to be performed two or four weeks. Each group was divided into three subgroups according daily application of enemas containing saline, curcumin at 50 mg/kg/day or 200 mg/kg/day. Colitis was diagnosed by histological analysis. Claudin-3 and occludin were determined by immunohistochemistry. The tissue content of claudin-3 and occludin were quantified by computer-assisted image analysis. MannWhitney, Student t and ANOVA tests were used to analyze the results establishing the level of significance of 5% for both (p<0.05). Results: Curcumin at both concentrations reduces the inflammation and preserves the tissue content of the proteins claudin-3 and occludin, which was related to the concentration used and to the time of the intervention. Conclusion:The application of enemas with curcumin reduces inflammation and preserves the tissue content of the proteins claudin-3 and occludin in the colonic mucosa devoid from the fecal stream.

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confidence: 99%

Claudin-3 and occludin content in the glands of colonic mucosa devoid from fecal stream submitted to topical intervention with oil extract of Curcuma longa

et al. 2017

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“…Активні форми кисню (АФК), які у фізіологічних концентраціях необхідні для нормального функціонування клітин, в надлишковій кількості викликають пошкодження біологічних молекул та структур [1,2]. В основі патогенезу переваж-ної більшості захворювань лежить оксидативне пошкодження клітин, ослаблення системи антиоксидантного захисту та порушення про-/антиоксидантного балансу в бік переважання процесів оксидації [1,8].…”

Section: вступunclassified

“…Вона не пов'язана зі здатністю впливати на метаболічні процеси, що забезпечують про-/антиоксидантний баланс в умовах цілісного організму, а зумовлена безпосередньою дією на АФК, нерадикальні індуктори та проміжні продукти вільнорадикального окислення. Гідроксильні (ОН) радикали є найбільш реакційно здатними і завдають найбільшої шкоди клітинним мембранам і ДНК, викликаючи загибель і мутації клітин [2]. Тому антирадикальну активність речовин зазвичай оцінюють за здатністю поглинати гідроксильні радикали.…”

Section: вступunclassified

“…Ефективність антирадикальної активності дослідних безклітинних екстрактів (БКЕ) та метабіотика (HF) визначали за їх здатністю поглинати ОН-радикали, що генеруються у системі залізо (Fe) − етилендиамінтетраацетат (ЕДТА), перекис водню (Н 2 О 2 ) і L-аскорбат, тим самим пригнічувати руйнування ОН-радикалами дезоксирибози (ДОР) [5]. Інкубаційне середовище містило 20 мМ КН 2 Для визначення маси сухого залишку 100 мкл екстракту поміщали у термостат і висушували за температури 105 ± 3 °С впродовж ~30 хв до отримання постійної ваги.…”

Section: матеріали та методи дослідженняunclassified

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In Vitro Anti-Radical Activity of Bifidobacterium Bifidum and Lactobacillus Reuteri Cell-Free Extracts

Knysh

Nikitchenko

2020

Act. Probl. of the Modern Med.

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Probiotic microorganisms are known to be able to exhibit antioxidant activity. Most of beneficial probiotics effects are due to the action of their derivatives (structural components and metabolites). The aim of the work was to investigate the antiradical activity of cell-free extracts containing bacterial derivatives of Bifidobacterium bifidum 1 and Lactobacillus reuteri DSM 17938 probiotic strains, by their hydroxyl radical scavenging activity in the model system of their generation. Cell-free extracts were obtained from disintegrators and cultures of probiotic bacteria cultured in the disintegrators of their own cells. A metabiotic HYLAK FORTE was chosen as the reference drug. At a concentration 20% vol. in the incubation medium the hydroxyl radical scavenging by L. reuteri cell-free extracts was at a level of 75–90 %, and the hydroxyl radical scavenging by B. bifidum cell-free extracts was at a level of 50–60%. The metabiotic provided the same level of the hydroxyl radical scavenging activity when its content in the incubation medium was lower in 1.5 – 2.5 times. Extracts obtained from disintegrators showed a more pronounced antiradical activity than extracts from probiotic cultures. Based on the weight of the dry residue, concentrations for 50% hydroxyl radical scavenging (ІС50) by extracts and metabiotic were calculated. According to this indicator, L. reuteri extract obtained from disintegrate was the most effective (IC50 = 1.57 mg/ml). Other investigated derivative-containing products were arranged in decreasing order of antiradical activity as follows: B. bifidum extract from disintegrate (IC50 = 1, 64 mg/ml) > B. bifidum extract from culture (IC50 = 1, 75 mg/ml) > L. reuteri extract from culture (IC50 = 1, 86 mg/ml) > HYLAK FORTE (IC50 = 11, 03 mg/ml). Thus, all the studied extracts showed antiradical activity with respect to the most reactive hydroxyl radical. The obtained results encourage further study of the antioxidant properties of L. reuteri and B. bifidum cell-free extracts in order to elucidate the mechanisms of their biological activity and justify the appropriateness of their therapeutic use.

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“…[17][18][19] Son çalışmalar IMA düzeylerinin inflamasyon sürecinde arttığını ve IMA'nın oksidatif stresin bir belirteci olarak gösterilmeye başlandığını rapor etmektedir. [20][21][22] Bilgilerimiz dahilinde, literatürde IMA düzey-leri ile nekrotizan periodontal hastalıklar arasındaki ilişkiyi inceleyen bir çalışma bulunmamaktadır. Bu ön çalışma ile bu ilişki değerlendirilmeye çalışılmıştır.…”

Section: Tartiġmaunclassified

Nekroti̇zan Peri̇odontal Hastaliklarda Serum İskemi̇k Modi̇fi̇ye Albümi̇n Sevi̇yeleri̇ni̇n Değerlendi̇ri̇lmesi̇: Ön Çalişma

Tayman¹,

Kurgan²,

Önder³

et al. 2016

Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi

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Amaç: Albümin, primer olarak karaciğerden sentezlenen majör bir plazma proteinidir. İskemi, hipoksi, asidozis, süperoksid-radikal yaralanmaları gibi birçok durum, albüminin metal iyon bağlama kapasitesinde azalmaya ve metabolik olarak farklı protein üretimine neden olmaktadır. Bu değişim, ölçülebilir bir değişim olup, iskemik modifiye albümin (IMA) olarak bilinir. Serumdaki artmış iskemik modifiye albümin seviyeleri, C-reaktif protein (CRP) ve interlökin-6 (IL-6) seviyeleri ile anlamlı derecede ilişkili bulunmuştur. Bu ön çalışmanın amacı, nekrotizan ülseratif periodontal hastalıklarda IMA seviyelerini, bu seviyelerin CRP ve IL-6 seviyeleri ile ilişkisini ve bu değerlerin periodontal tedaviden sonra nasıl değiştiğini incelemektir. Gereç veYöntem: Bu çalışmada, nekrotizan ülseratif gingivitis (NUG) (1 vaka) ve nekrotizan ülseratif periodontitisin (NUP) akut ve subakut fazlarında olan hastaların (2 vaka) başlangıçtaki ve cerrahi olmayan periodontal tedavi sonrası 1. aydaki ve sistemik ve periodontal olarak sağlıklı kontrol bireylerinin (3 vaka) başlangıçtaki serum IMA, CRP ve IL-6 seviyeleri değerlendirilmiştir. Bulgular: Başlangıçtaki CRP ve IL-6 seviyeleri NUP'un akut fazında olan hastada NUP'un subakut fazında olan hastaya ve NUG hastasına göre daha yüksek bulunmuştur. CRP ve IL-6 seviyeleri nekrotizan hastalıklarda kontrol bireylerine göre daha yüksek bulunmuştur. Başlangıçtaki IMA seviyelerinin NUP hastalarında, NUG hastasına göre daha yüksek olduğu gözlenirken; en düşük değerler kontrol bireylerinde bulunmuştur. IMA, CRP ve IL-6 değerlerinin her üçü de tedavi ile birlikte kontrol seviyelerine düşüş göstermiştir. Sonuç: Bu çalışmanın sonuçları göz önüne alınırsa, nekrotizan periodontal hastalıklarda artan IMA seviyelerinin, hastalık aktivite kriteri olarak kullanılabileceğini söyleyebiliriz. Anahtar kelimeler: Nekrotizan ülseratif gingivitis, nekrotizan ülseratif periodontitis, iskemik modifiye albümin ABSTRACTAim: Albumin is a major plasma protein synthesized primarily in the liver. Various conditions; such as ischemia, hypoxia, acidosis, superoxide-radical injury cause reduction of the binding capacity of albumin for metals and cause metabolic production of different proteins. This variation is measurable and it's known as ischemia modified albumin (IMA). Increased serum levels of IMA were significantly associated with serum levels of C-reactive protein (CRP) and interleukin-6 (IL-6). The aim of this preliminary study is to investigate the IMA levels, and the relationship between the levels of IMA and CRP and also IL-6 in patients with necrotizing ulcerative periodontal diseases. Case Reports: In this study, serum IMA, CRP and IL-6 levels were determined by ELISA method in patients with NUG, with acute and sub acute phases of NUP, and in systemically and periodontally healthy control subjects, before and after 1 month of non-surgical periodontal treatment. Results: Baseline CRP and IL-6 levels were higher inpatient with acute phase of NUP than the patients with sub acute phase of NUP, and NUG. At the same ti...

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Effect of Curcumin Against Oxidation of Biomolecules by Hydroxyl Radicals

Cited by 49 publications

References 48 publications

Claudin-3 and occludin content in the glands of colonic mucosa devoid from fecal stream submitted to topical intervention with oil extract of Curcuma longa

Claudin-3 and occludin content in the glands of colonic mucosa devoid from fecal stream submitted to topical intervention with oil extract of Curcuma longa

In Vitro Anti-Radical Activity of Bifidobacterium Bifidum and Lactobacillus Reuteri Cell-Free Extracts

Nekroti̇zan Peri̇odontal Hastaliklarda Serum İskemi̇k Modi̇fi̇ye Albümi̇n Sevi̇yeleri̇ni̇n Değerlendi̇ri̇lmesi̇: Ön Çalişma

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