Effect of Curcumin on Oxidative Stress in a Model of Turpentine Induced Acute Experimental Inflammation

Abstract

Curcumin, a natural phenolic compound is an anti-tumor agent with anti-inflammatory and anti-oxidant properties. The aim of this research was to evaluate oxidative stress levels, the antioxidant activity and Curcumin concentrations by high performance liquid chromatography (HPLC) in an acute experimental inflammation induced by Turpentine oil (intramuscular 0.6 mg kg -1 body weight) and to compare a prophylactic versus a therapeutic regimen of Curcumin (oral suspension of 150 mg Curcumin kg -1 rat weight). Sixteen adult male Wistar rats were assigned to four groups: Control, Group I (Curcumin only), Group II (Curcumin administration, then induced inflammation after 1 hour) and Group III (induced inflammation then Curcumin administration after 2 hours). Oxidative stress was assessed by measuring serum malondialdehide and carbonylated proteins, while systemic and local total antioxidant capacity was determined by ABTS. Local tissue changes (muscle, kidney, liver) were analysed using histopathology. Results showed that acute inflammation significantly increased lipid peroxidation in Groups II and III compared to Control and Group I. A significantly reduced total antioxidant capacity (ATBS) was present in serum and kidney in Group II, also in muscle and kidney in Group III. ABTS levels were significantly increased only in the liver tissue of the animals in Groups II and III with induced inflammation as compared to Group I. This study proved the potential of Curcumin in reducing oxidative stress in both prophylactic and therapeutic regimens.

Curcumin in Liver Diseases: A Systematic Review of the Cellular Mechanisms of Oxidative Stress and Clinical Perspective

“…TCDD also increased liver levels of GSH, CuZn-SOD and GSH-Px [ 77 ]. Coneac et al found that curcumin is able to reduce oxidative stress in acute experimental inflammation produced by Turpentine oil [ 78 ]. Dai et al has confirmed that curcumin could ameliorate reduced L02 cell viability, prevent oxidative stress and inhibit the rises of SOD activity and GSH level.…”

"High-dose statin prior to primary percutaneous coronary intervention reduces oxidative stress burden in patients with acute ST-elevation myocardial infarction "

“…Reactive oxygen species degrade polyunsaturated lipids, forming malondialdehyde (MDA). This stable end-product aldehyde is used as a biomarker to measure the level of oxidative stress in an organism [13][14][15].…”