Effect of curcumin on sperm parameters after the cryopreservation

Karakuş, Fatma Nur; Kuran, Sibel Bulgurcuoğlu; Solakoğlu, Seyhun

doi:10.1016/j.ejogrb.2021.10.027

Cited by 25 publications

(15 citation statements)

References 68 publications

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“…In the last decades, the study of in vitro incubation of spermatozoa with various antioxidants, including melatonin, resveratrol, penicillamine, hydroxytyrosol, vitamin B12, hypotaurine, and curcumin, has been an exciting research subject (Alamo et al, 2019; Hosseinabadi et al, 2020; Karakus et al, 2021; Kedechi et al, 2017; Pariz et al, 2019; Pons‐Rejraji et al, 2021; Uribe et al, 2020). L‐Proline (Proline) is a membrane‐permeable osmoprotectant and antioxidant that protects cells against cryopreservation‐induced damages (Bottrel et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Beneficial effect of L‐ Proline supplementation on the quality of human spermatozoa

Moradi

Hashemian

et al. 2022

Andrologia

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L-Proline is a natural anti-oxidative and osmoprotectant agent, playing a versatile role in cell metabolism and physiology. The present study aimed to explore the antioxidant effects of L-Proline on human sperm function during incubation. Thirty healthy, normozoospermic men (27-40 years) were enrolled. Sperm samples were incubated in an unsupplemented sperm medium (control group), or supplemented with L-Proline (1, 2 and 4 mmol/L) to evaluate its effect during 0, 1, 4 and 24 h of incubation. Sperm were assessed in terms of motility, viability, morphology, chromatin and DNA integrity. Moreover, the levels of reactive oxygen species (ROS), malondialdehyde (MDA), and total antioxidant capacity (TAC) were determined in the sperm medium. The results indicated that 2 mmol/L of L-Proline significantly improved the maintenance of sperm motility, viability, normal morphology, chromatin and DNA integrity, and TAC levels compared to the control group during 24 h of incubation (p < 0.05). However, 1 and 4 mmol/L of L-Proline could not significantly preserve sperm parameters, chromatin quality, and antioxidant status during different incubation times compared to the control group (p > 0.05). Collectively, the inclusion of L-Proline (2 mmol/L) in the human sperm medium maintains sperm parameters and chromatin quality probably by modulating the oxidative status.

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Section: Discussionmentioning

confidence: 99%

Beneficial effect of L‐ Proline supplementation on the quality of human spermatozoa

Moradi

Hashemian

et al. 2022

Andrologia

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“…DNA integrity was assessed by acridine orange (AO) as reported by Ammar et al (2020) and Karakus et al (2021) with appropriate modifications. Briefly, a 50 μL sperm aliquot was spread to a clean slide and fixed with anhydrous methanol‐glacial acetic acid (3:1) for more than 2 h. Then 20 μL of AO working solution was spread to the slide coated with the aliquot and incubated at 37°C for 30 min under dark and humidified conditions, followed by rinsing with HEPES and air‐drying away from light.…”

Section: Methodsmentioning

confidence: 99%

Phosphocreatine addition to extender enhances the quality and antioxidant capacity of cryopreserved boar sperm

Wang,

Guo,

et al. 2023

Reprod Domestic Animals

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Boar sperm are less resistant to drastic changes in the external environment during cryopreservation, mainly because their plasma membranes are rich in unsaturated fatty acids but lack cholesterol and are thus susceptible to lipid peroxidation caused by the attack of reactive oxygen species. This study evaluated the effect of adding phosphocreatine to cryopreservation extenders on boar sperm quality and antioxidant capacity. Different concentrations (0, 5.0, 7.5, 10.0 and 12.5 mmol/L) of phosphocreatine were added to the cryopreservation extender. After thawing, sperm were analysed for morphological parameters, kinetic parameters, acrosome integrity, membrane integrity, mitochondrial activity, DNA integrity and antioxidant enzyme activity. The results showed that 10.0 mmol/L phosphocreatine samples enhanced the boar sperm motility, viability, average path velocity, straight‐line velocity, curvilinear velocity and beat cross frequency after cryopreservation and reduced the malformation rate compared to the control group (p < .05). The acrosome integrity, membrane integrity, mitochondrial activity and DNA integrity of boar sperm were higher than those of the control group after adding 10.0 mmol/L phosphocreatine to the cryopreservation extender (p < .05). Extenders containing 10.0 mmol/L phosphocreatine maintained high total antioxidant capacity; elevated the activities of catalase, glutathione peroxidase and superoxide dismutase; reduced malondialdehyde and H2O2 content (p < .05). Therefore, adding phosphocreatine to the extender is potentially beneficial for boar sperm cryopreservation at an optimal 10.0 mmol/L concentration.

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“…DNA integrity was assessed by acridine orange (AO) as reported by Ammar et al (2020) and Karakus et al (2021) with appropriate modifications. Briefly, from each sample, 100 μl was taken, processed by centrifugation (5 min, 290× g ), and the supernatant was discarded.…”

Section: Methodsmentioning

confidence: 99%

Exogenous spermidine effectively improves the quality of cryopreserved boar sperm

Li,

Wang,

Guo

et al. 2023

Animal Science Journal

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Boar sperm are less resistant to the dramatic environmental changes that occur during in vitro preservation. Spermidine has various physiological functions including the anti‐oxidative effect. The main objective of this study was to clarify whether spermidine could protect boar sperm from the attack of reactive oxygen species under cryopreservation treatment. We set the concentrations of spermidine at 0, 2, 4, 6, and 8 mmol/L and evaluated the effects of spermidine on sperm motility, viability, malformation rates, kinetic parameters, membrane integrity, mitochondrial activity, DNA integrity, H2O2 content, malondialdehyde content, total antioxidant capacity, and antioxidant enzyme activity. Finally, the effects of spermidine on the sperm fertility were assessed by artificial insemination. The results showed that spermidine improved various physiological parameters of sperm in a dose‐dependent manner. The quality and antioxidant capacity of sperm cryopreserved with 6 mmol/L spermidine were significantly less reduced (P < 0.05), and the contents of malformation rate, H2O2, and malondialdehyde content were significantly decreased (P < 0.05). The significant increase in the number of litters indicated the possibility that spermidine had important practical value in pig reproduction (P < 0.05). Therefore, the addition of appropriate concentrations of spermidine to cryopreservation extenders may effectively improve the quality of boar sperm for in vitro preservation.

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Effect of curcumin on sperm parameters after the cryopreservation

Cited by 25 publications

References 68 publications

Beneficial effect of L‐ Proline supplementation on the quality of human spermatozoa

Beneficial effect of L‐ Proline supplementation on the quality of human spermatozoa

Phosphocreatine addition to extender enhances the quality and antioxidant capacity of cryopreserved boar sperm

Exogenous spermidine effectively improves the quality of cryopreserved boar sperm

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