Effect of Cycloheximide, Gamma Irradiation, and Phosphorus Deficiency on Root Pressure Exudation in Tobacco

Wallace, A.; Ashcroft, R. T.; Leo, Monica; Wallace, G. A.

doi:10.1104/pp.45.3.300

Cited by 9 publications

(3 citation statements)

References 12 publications

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“…Cycloheximide, an inhibitor of protein synthesis, and roscovitine, a cyclin-dependent kinase inhibitor, were selected because they were previously tested in plants and mammalian cells (Wallace et al , 1970; Planchais et al , 1997). They both inhibited root growth in a concentration-dependent manner (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

ATP-competitive mTOR kinase inhibitors delay plant growth by triggering early differentiation of meristematic cells but no developmental patterning change

Montané

Menand

2013

Journal of Experimental Botany

175

217

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The TOR (target of rapamycin) protein, a large phosphatidylinositol 3-kinase-like protein kinase (PIKK) that is conserved in eukaryotes and is a central regulator of growth and metabolism. The analysis of function of TOR in plant growth and development has been limited by the fact that plants are very poorly sensitive to rapamycin. As the kinase domain of TOR is highly conserved, this study analysed the dose-dependent effect of three sets of first- and second-generation ATP-competitive inhibitors (called asTORis for active-site TOR inhibitors) recently developed for the human TOR kinase on Arabidopsis thaliana growth. All six asTORis inhibited plant root growth in a dose-dependent manner, with 50% growth inhibitory doses (GI50) of <10 μM and <1 μM for the first- and second-generation inhibitors, respectively, similarly to the values in mammalian cells. A genetic approach further demonstrated that only asTORis inhibited root growth in an AtTOR gene-dosage-dependent manner. AsTORis decreased the length of: (i) the meristematic zone (MZ); (ii) the division zone in the MZ; (iii) epidermal cells in the elongation zone; and (iv) root hair cells. Whereas meristematic cells committed to early differentiation, the pattern of cell differentiation was not affected per se. AsTORis-induced root hair growth phenotype was shown to be specific by using other growth inhibitors blocking the cell cycle or translation. AsTORis dose-dependent inhibition of growth and root hairs was also observed in diverse groups of flowering plants, indicating that asTORis can be used to study the TOR pathway in other angiosperms, including crop plants.

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Section: Resultsmentioning

confidence: 99%

ATP-competitive mTOR kinase inhibitors delay plant growth by triggering early differentiation of meristematic cells but no developmental patterning change

Montané

Menand

2013

Journal of Experimental Botany

175

217

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“…These roots reduce little of the NO3-that is absorbed, and most of it is transported to the stems and leaves (3). As with tobacco (30,31), cucumber roots depleted of NO3-respond to a new supply of this ion with increased rates of exudation and salt transport. These properties make the cucumber root ideal for studying ion transport to the xylem.…”

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confidence: 99%

Accumulation and Radial Transport of Ions from Potassium Salts by Cucumber Roots

Cooil¹

1974

Plant Physiol.

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Accumulation of K+ is insensitive to the anion supplied with it at a solution concentration below 1 mM. Rates of K+ transport to the xylem from the same solutions are, however, dependent upon the anion present and decrease in the order NO3->> Cl-> SOI2-. Parallel effects on rates of exudation and anion transport result from kind and concentration of anion supplied and time of exposure to the solution. When high K salt concentrations are used, only linear relationships are found between solution concentrations and transport rates. However, ion concentration in the exudate increases more than external solution concentration, while exudation rate is unaffected. It is suggested that some of the ions transported are from compartments within the cells. At high solution concentrations KNO3 results in more exudation and in higher ion concentration in the exudate than is found with KCI.The processes involved in transport of ions from the external medium into the xylem of roots continue to evoke controversy and have been discussed in several recent reviews (1,18,19,27). Liittge and Laties (22) Cucumber roots can be induced to exude from the xylem profusely. These roots reduce little of the NO3-that is absorbed, and most of it is transported to the stems and leaves (3). As with tobacco (30, 31), cucumber roots depleted of NO3-respond to a new supply of this ion with increased rates of exudation and salt transport. These properties make the cucumber root ideal for studying ion transport to the xylem.This report compares patterns of accumulation and radial transport in cucumber roots of K salt ions supplied at concentrations below 1 mm, and transport at concentrations of 0.5 to 10 mM. MATERIALS AND METHODSPlant Culture. Seeds of Cucumnis sativus L. cv. Burpeeana Hybrid were soaked in deionized water with continuous aeration for 24 hr, then planted in moist vermiculite and allowed to germinate in the dark for about 65 hr at 23 to 25 C. The seedlings were next exposed to indirect light for about 24 hr. Single plants were then transferred to 4-liter glass jars of continuously areated nutrient solution consisting of 1 mM Ca (N03)

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“…Ob die Hemmung des Leucineinbaus in die loslichen Proteine nadi Iangerer Vorinkubation mit CH und 2-TU (Abb. 2) ratsadilich auf eine Hemmung der Proteinsynthese schlieBen laBt oder moglidierweise darauf beruht, daB der Aufnahmemechanismus der Blattzellen (Transportsystem) fiir bestimmte Substanzen -im vorliegenden Fall fur Leucin -gesdiadigt wird(Literatur siehe ELLIS und MAC DoNALD 1970, WALLACE et al 1970) laBt sich aus den vorliegenden Ergebnissen nidit schliissig ableiten. Sollte das erstere zutreffen, so konnte der nach…”

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Die Wirkung von Cycloheximid und 2‐Thiouracil auf die Proteinsynthese in Blättern von Hyoscyamus niger im Zusammenhang mit der Induktion der Blütenbildung

Klautke¹,

Rau²

1973

Berichte der Deutschen Botanischen Gesellschaft

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Effect of Cycloheximide, Gamma Irradiation, and Phosphorus Deficiency on Root Pressure Exudation in Tobacco

Cited by 9 publications

References 12 publications

ATP-competitive mTOR kinase inhibitors delay plant growth by triggering early differentiation of meristematic cells but no developmental patterning change

ATP-competitive mTOR kinase inhibitors delay plant growth by triggering early differentiation of meristematic cells but no developmental patterning change

Accumulation and Radial Transport of Ions from Potassium Salts by Cucumber Roots

Die Wirkung von Cycloheximid und 2‐Thiouracil auf die Proteinsynthese in Blättern von Hyoscyamus niger im Zusammenhang mit der Induktion der Blütenbildung

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