Effect of cysteine on inducible synthesis of β-galactosidase in Escherichia coli

Kari, C.; Nagy, Z.; Hernádi, F.

doi:10.1016/0006-2952(71)90319-4

Biochemical Pharmacology

1971

DOI: 10.1016/0006-2952(71)90319-4

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Effect of cysteine on inducible synthesis of β-galactosidase in Escherichia coli

C. Kari

Z. Nagy

F. Hernádi

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1986

1996

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Cited by 2 publications

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“…We studied the repressive effect of different amino acids on protease production by Pseudomonas fluorescens NC3 and observed cysteine to be the most effective (B. H. Himelbloom, Ph.D. thesis, North Carolina State University, Raleigh, 1985). It has been shown to inhibit growth and protein synthesis in procaryotes (2,8,11,14). Also, extracellular protease and a-amylase production by Aspergillus niger (18) and Bacillus subtilis (15), respectively, is inhibited by cysteine.…”

mentioning

confidence: 99%

Effects of cysteine on growth, protease production, and catalase activity of Pseudomonas fluorescens

Himelbloom¹,

Hassan²

1986

Appl Environ Microbiol

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confidence: 99%

Effects of cysteine on growth, protease production, and catalase activity of Pseudomonas fluorescens

Himelbloom¹,

Hassan²

1986

Appl Environ Microbiol

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Thiols, rec A induction and radiosensitivity in Escherichia coli

Näslund

Anderstam

Granath

et al. 1996

International Journal of Radiation Biology

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Induction by gamma-radiation, UV radiation or hydroxyurea of RecA gene product synthesis in Escherichia coli, monitored as beta-D-galactosidase in recA-lacZ fusion strains, was shown to be inhibited if 2-mercaptoethylamine (MEA) was added before treatment with the inducing agents. If cysteine (Cys) at low concentrations was added at the same time as MEA it counteracted the action of MEA. The effect of MEA may be described as a competitive inhibition of an inducing or conducting effect of Cys. In E. coli GE499 (uvrA+), complete inhibition by 30-mmol dm-3 MEA of recA induction was associated with about five times higher radio-resistence. Both of these effects of MEA were completely reversed by 0.3-mmol dm-3 Cys. As shown in parallel experiments with E. coli GE500 (uvrA-), these effects of MEA and Cys were shown to be independent of excision-repair proficiency. Treatment of bacteria with MEA and/or Cys was shown not to lead to increased intracellular concentrations of these thiols. Instead, treatment with them appeared to provoke conspicuous increases in glutathione levels, which are, however, probably not directly involved in the studied action of MEA and Cys.

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Effect of cysteine on inducible synthesis of β-galactosidase in Escherichia coli

Cited by 2 publications

References 9 publications

Effects of cysteine on growth, protease production, and catalase activity of Pseudomonas fluorescens

Effects of cysteine on growth, protease production, and catalase activity of Pseudomonas fluorescens

Thiols, rec A induction and radiosensitivity in Escherichia coli

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