Effect of cytokinin on chloroplast cyclic differentiation in cultured tobacco cells

Seyer, Patrick; Marty, Danielle; Lescure, Anne‐Marie; Péaud‐Lenoël, C.

doi:10.1016/0045-6039(75)90040-8

Cited by 48 publications

(4 citation statements)

References 13 publications

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“…It is known that the changes in chlorophyll content is associated with the changes in the chloroplasts (Evans, 1984). The important factors that control chloroplast differentiation area are: Genetic information present in plastids which contain the chloroplast DNA (Kirk and Tilney-Basset, 1978), (2) Cytokinins have been shown to control chloroplast differentiation independently of their action on cell division (Seyer et al, 1975) and (3) inorganic salts (iron, magnesium, copper, potassium and ammonium salts) play important roles in the synthesis or metabolism of chlorophyll in plants (Steward,1963).…”

Section: Leaves Chlorophyll Contentmentioning

confidence: 99%

EFFECT OF GAMMA IRRADIATION,METHYL METHANE SULPHONATE AND THEIR COMBINATIONS ON GROWTH, FLOWERING AND INDUCED VARIABILITY IN Tagetes erecta, L.

Badr

El-Shennawy²,

Mostafa³

et al. 2000

Journal of Plant Production

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Seeds of Tagates erecta. were irradiated with different doses of gamma-rays ( 0,5,10 and 15 kr.), other part of seeds was treated with different levels of MMS ( 0.0, 0.1, 0.2, 0.3,0.4, 0.5 and 1.0%) . Also there were combined treatments between the two mutagens.. Intermediate doses of gamma-rays (10kr.) gave the lowest germination percentages in both seasons. There were no significant differences among the different treatments of gamma-rays in M1-and M2-generations of both seasons-on the plant height. All doses of gamma-rays delayed the flowering date in all generations for both seasons. Except the M2-generation of the first season, gamma-rays showed significant effect on the number of flowers in all generations. 5 kr. in the M1generations of both seasons had the largest average number of inflorescences, but in the M2-generation of the second season, it had the lowest one. Low doses of gammarays (5 kr.) caused smaller flowers than normal in the M2-generation of the first season.Low concentrations of MMS (0.1%) caused a stimulating effect on seed germination in both generations. But the high concentration (1.0%) caused death of seeds ( lethal dose). Different treatments of MMS showed no significant differences in M1-generation in both seasons on the plant height. High concentration of MMS (0.5%) caused albino chlorophyll mutant in the seedlings in the M2-generation of the first season. Low concentration of MMS (0.2%) delayed the flowering date in all generations with one exception in the M2-generation of the second season, where 0.5% was the latest one. Low concentration of MMS (0.1%) gave the largest flower number in the M1-generation for both seasons but the high concentration (0.5%) gave the largest one in the M2-generation of the first season.The combined treatments were more efficient than the single ones on seed germination . The lethal dose ranged between the doses 1.0% MMS + 0. Kr. gamma-rays to 1.0% MMS + 15 kr. gamma-rays. Generally, 0.5% MMS + 5 kr. gamma-rays gave the largest number of inflorescences in all generations except in the M2-generation of the second season. Many different petals shape were obtained from the different combined treatments. Seeds of M1-plants treated with 10 kr. + 0.3% resulted in a mutation in stalk shape.

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Section: Leaves Chlorophyll Contentmentioning

confidence: 99%

EFFECT OF GAMMA IRRADIATION,METHYL METHANE SULPHONATE AND THEIR COMBINATIONS ON GROWTH, FLOWERING AND INDUCED VARIABILITY IN Tagetes erecta, L.

Badr

El-Shennawy²,

Mostafa³

et al. 2000

Journal of Plant Production

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“…An absolute cytokinin requi'rement? By most accounts a cytokinin is required for cultured cells to accumulate and maintain ChI (5,18,21,22,23,24,25) as well as to develop structurally normal chloroplasts (25,26) which contain a full photosystem complement (23). In at least one report (13), a cytokinin was not absolutely required for greening but the inclusion of 0.1 mg/l kinetin resulted in a 60% increase in ChI.…”

Section: General Requirements For Chi Synthesis and Accumulationmentioning

confidence: 93%

“…All three systems allow the plastids a relatively slow transition compared to that seen with etiolated whole plant tissue. Before greening the plastids almost ~lways contain a large amount of starch and few if any lamellae (21,25,42,43,44,45). One wonders if the large starch content obscures the lamellae which might exist in two of these reports (44,45).…”

Section: Structural Changes During Greening Of Cell Culturesmentioning

confidence: 98%

Aspects of Photosynthetic Plant Tissue Cultures

Horn

Widholm

1984

Applications of Genetic Engineering to Crop Improvement

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“…However, chlorophyll synthesis and early appearance of photosynthetic membranes in plastids are observed only when a cytokinin is added to the culture medium (14,15). In the cell line that we cultivate under continuous white light, cell multiplication readily occurs in the absence of any exogenous cytokinin.…”

Section: Introductionmentioning

confidence: 99%

Cytokinins modulate the expression of genes encoding the protein of the light-harvesting chlorophyll a/b complex

Serve¹,

Axelos²,

Péaud‐Lenoël³

1985

Plant Mol Biol

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Tobacco cell suspension cultures responded to cytokinins (for instance kinetin) by full chloroplast differentiation. The hormone had the effect of stimulating the appearance of a few prominent plastid proteins. Synthesis of the light-harvesting chlorophyll a/b-binding protein (LHCP) in response to kinetin was noteworthy (Axelos M. et al.: Plant Sci Lett 33:201-212, 1984).Poly(A) +RNAs were prepared from cells grown in the presence of or without added kinetin. Poly(A) + RNA recovery and translation activity were not quantitatively altered by the hormone treatment. In vitro translation of polyadenylated mRNA into precursor polypeptides of LHCP (pLHCP) was quantified by immunoprecipitation and SDS-PAGE fractionation of p L H C P immunoprecipitates: p L H C P -m R N A translating activity was found to be stimulated in parallel to mature LHCP accumulation by kinetin-induced cells.Dot-blot and northern-blot hybridizations of poly(A)+RNA were carried out, using as a probe a pea LHCP-cDNA clone (Broglie R. et al.: Proc Natl Acad Sci USA 78:7304-7308, 1981). A ten-fold increase of the level of pLHCP-encoding sequences was observed in poly(A)+RNA prepared from 9-d kinetin-stimulated cells, compared to control cells. Oligo(dT)-cellulose-excluded RNA fractions exhibited very low hybridization levels, in the same ratios as those obtained with poly(A)+RNA.Thus, the expression of LHCP-gene activity, in response to kinetin addition to tobacco cell suspension cultures, is regulated by the level of pLHCP-encoding mRNA rather than by translational or post-translational controls.

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Effect of cytokinin on chloroplast cyclic differentiation in cultured tobacco cells

Cited by 48 publications

References 13 publications

EFFECT OF GAMMA IRRADIATION,METHYL METHANE SULPHONATE AND THEIR COMBINATIONS ON GROWTH, FLOWERING AND INDUCED VARIABILITY IN Tagetes erecta, L.

EFFECT OF GAMMA IRRADIATION,METHYL METHANE SULPHONATE AND THEIR COMBINATIONS ON GROWTH, FLOWERING AND INDUCED VARIABILITY IN Tagetes erecta, L.

Aspects of Photosynthetic Plant Tissue Cultures

Cytokinins modulate the expression of genes encoding the protein of the light-harvesting chlorophyll a/b complex

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